Abstract: In vitro storage and processing of animal semen represents a risk factor to spermatozoa vitality, potentially leading to reduced fertility. A variety of substances isolated from natural sources may exhibit protective or antioxidant properties on the spermatozoon, thus extending the lifespan of stored ejaculates. This study compared the ability of different concentrations of the Salvia officinalis extract on the motility, mitochondrial activity, viability and reactive oxygen species (ROS) production by bovine spermatozoa during different time periods (0, 2, 6 and 24 h) of in vitro culture. Spermatozoa motility was assessed using the Computer-assisted sperm analysis (CASA) system. Cell viability was examined using the metabolic activity MTT assay, the eosin-nigrosin staining technique was used to evaluate the sperm viability and ROS generation was quantified using luminometry. The CASA analysis revealed that the motility in the experimental groups supplemented with 0.5-2 µg/mL Salvia extract was significantly lower in comparison with the control (P
Abstract: The Algeria by its location offers a rich and diverse
vegetation. A large number of aromatic and medicinal plants grow
spontaneously. The interest in these plants has continued to grow in
recent years. Their particular properties due to the essential oil
fraction can be utilized to treat microbial infections. To this end, and
in the context of the valuation of the Algerian flora, we became
interested in the species of the family Lamiaceae which is one of the
most used as a global source of spices. The plant on which we have
based our choice is a species of sage "Salvia officinalis" from the
Isser localized region within the province of Boumerdes. This work
focuses on the study of the antimicrobial activity of essential oil
extracted from the leaves of Salvia officinalis. The extraction is
carried out by essential oil hydrodistillation and reveals a yield of
1.06℅. The study of the antimicrobial activity of the essential oil by
the method of at aromatogramme shown that Gram positive bacteria
are most susceptible (Staphylococcus aureus and Bacillus subtilis)
with a strong inhibition of growth. The yeast Candida albicans
fungus Aspergillus niger and have shown moderately sensitive.
Abstract: This study presents an attempt to evaluate the
antioxidant potential and antimicrobial activity of methanolic extract,
and essential oils prepared from the leaves of sage (Salvia officinalis
L.). The content of polyphenol in the methanolic extracts from the
leaves of Salvia officinalis was determined spectrophotometrically,
calculated as gallic acid and catechin equivalent. The essential oils
and methanol extract were also subjected to screenings for the
evaluation of their antioxidant activities using 2, 2-diphenyl-1-
picrylhydrazyl (DPPH) test. While the plant essential oils showed
only weak antioxidant activities, its methanol extract was
considerably active in DPPH (IC50 = 37.29 μg/ml) test. Appreciable
total polyphenol content (31.25 mg/g) was also detected for the plant
methanol extract as gallic acid equivalent in the Folin–Ciocalteu test.
The plant was also screened for its antimicrobial activity and good to
moderate inhibitions were recorded for its essential oils, and
methanol extracts against most of the tested microorganisms.
The present investigation revealed that this plant had rich source
of antioxidant properties. It is for this reason that sage has found
increasing application in food formulations.