Abstract: Diclofenac sodium, a member of the acetic acid family of non-steroidal anti-inflammatory drugs, is used to retard inflammation, arthritis pain and ankylosing spondylitis. The drug is known to cause severe injury in different tissues due to formation of reactive oxygen species. The present study is focused on the effect of different doses of diclofenac (4 mg/kg/body weight and 14 mg/kg/body weight on histoarchitecture of the liver from 7-28 days of the investigation. Diclofenac administration resulted in distorted hepatic degeneration and formation of wide areas in the form of sinusoidal gaps. Hepatic fibrosis noticed in different stages of investigation could be attributed to chronic inflammation and reactive oxygen species which results in deposition of extracellular matrix proteins. The abrupt degenerative changes observed during later stages of the experiment showed maximum damage to the liver, and there was enlargement of sinusoidal gaps accompanied by maximum necrosis in the tissues.
Abstract: The main cause of Alzheimer disease (AD) was
believed to be mainly due to the accumulation of free radicals owing
to oxidative stress (OS) in brain tissue. The mechanism of the
neurotoxicity of Aluminum chloride (AlCl3) induced AD in
hippocampus Albino wister rat brain tissue, the curative & the
protective effects of Lipidium sativum group (LS) water extract were
assessed after 8 weeks by attenuated total reflection spectroscopy
ATR-IR and histologically by light microscope. ATR-IR results
revealed that the membrane phospholipid undergo free radical
attacks, mediated by AlCl3, primary affects the polyunsaturated fatty
acids indicated by the increased of the olefinic -C=CH sub-band area
around 3012 cm-1 from the curve fitting analysis. The narrowing in
the half band width (HBW) of the sνCH2 sub-band around 2852 cm-1
due to Al intoxication indicates the presence of trans form fatty acids
rather than gauch rotomer. The degradation of hydrocarbon chain to
shorter chain length, increasing in membrane fluidity, disorder, and
decreasing in lipid polarity in AlCl3 group indicated by the detected
changes in certain calculated area ratios compared to the control.
Administration of LS was greatly improved these parameters
compared to the AlCl3 group. Al influences the Aβ aggregation and
plaque formation, which in turn interferes to and disrupts the
membrane structure. The results also showed a marked increase in
the β-parallel and antiparallel structure, that characterize the Aβ
formation in Al-induced AD hippocampal brain tissue, indicated by
the detected increase in both amide I sub-bands around 1674, 1692
cm-1. This drastic increase in Aβ formation was greatly reduced in the
curative and protective groups compared to the AlCl3 group and
approached nearly the control values. These results supported too by
the light microscope. AlCl3 group showed significant marked
degenerative changes in hippocampal neurons. Most cells appeared
small, shrieked and deformed. Interestingly, the administration of LS
in curative and protective groups markedly decreases the amount of
degenerated cells compared to the non-treated group. In addition, the
intensity of congo red stained cells was decreased. Hippocampal
neurons looked more/or less similar to those of control. This study showed a promising therapeutic effect of Lipidium
sativum group (LS) on AD rat model that seriously overcome the
signs of oxidative stress on membrane lipid and restore the protein
misfolding.
Abstract: Toxicity of copper (Cu), lead (Pb) and iron (Fe) to
Tilapia guinensis was carried out for 4 days with a view to
determining their effects on the liver and muscle tissues. Tilapia
guinensis samples of about 10 - 14cm length and 0.2 – 0.4kg weight
each were obtained from University of Calabar fish ponds and
acclimated for three (3) days before the experimental set up.
Survivors after the 96-hr LC50 test period were selected from test
solutions of the heavy metals for the histopathological studies.
Histological preparations of liver and muscle tissues were randomly
examined for histopathological lesions. Results of the histological
examinations showed gross abnormalities in the liver tissues due to
pathological and degenerative changes compared to liver and muscle
tissues from control samples (tilapia fishes from aquaria without
heavy metals). Extensive hepatocyte necrosis with chronic
inflammatory changes was observed in the liver of fishes exposed to
Cu solution. Similar but less damaging effects were observed in the
liver of fishes exposed to Pb and Fe. The extent of lesion observed
was therefore heavy metal-related. However, no pathologic changes
occurred in the muscle tissues.
Abstract: Current research is targeting new molecular
mechanisms that underlie non-alcoholic fatty liver disease (NAFLD)
and associated metabolic disorders like non-alcoholic steatohepatitis
(NASH). Forty New Zealand White rabbits have been used and fed a
high protein (HP) and energy diet based on grains and containing
11.76 MJ/kg. Boron added to 3 experimental groups’ drinking waters
(30 mg boron/L) as boron compounds. Biochemical analysis
including boron levels, and nuclear magnetic resonance (NMR) based
metabolomics evaluation, and mRNA expression of peroxisome
proliferator-activated receptor (PPAR) family was performed. LDLcholesterol
concentrations alone were decreased in all the
experimental groups. Boron levels in serum and feces were increased.
Content of acetate was in about 2x higher for anhydrous borax group,
at least 3x higher for boric acid group. PPARα mRNA expression
was significantly decreased in boric acid group. Anhydrous borax
attenuated mRNA levels of PPARγ, which was further suppressed by
boric acid. Boron supplementation decreased the degenerative
alterations in hepatocytes. Except borax group other boron groups did
not have a pronounced change in tubular epithels of kidney. In
conclusion, high protein and energy diet leads hepatocytes’
degenerative changes which can be prevented by boron
supplementation. Boric acid seems to be more effective in this
situation.
Abstract: The thyroid gland is the largest classic endocrine
organ that effects many organs of the body and plays a significant
role in the process of Metabolism in animals. The aim of this study
was to investigate the prevalence of thyroid disorders diagnosed by
ultrasound and microscopic Lesions of the thyroid during the
slaughter of apparently healthy One Humped Camels (Camelus
dromedarius) in Iran. Randomly, 520 male camels (With an age
range of 4 to 8 years), were studied in 2012 to 2013. The Camels’
thyroid glands were evaluated by sonographic examination. In both
longitudinal and transverse view and then tissue sections were
provide and stained with H & E and finally examined by light
microscopy. The results obtained indicated the following:
hyperplastic goiter (21%), degenerative changes (12%), follicular
cysts (8%), follicular atrophy (4%), nodular hyperplasia (3%),
adenoma (1%), carcinoma (1%) and simple goiter colloid (1%).
Ultrasound evaluation of thyroid gland in adenoma and carcinoma
showed enlargement and irregular of the gland, decreased
echogenicity, and the heterogeneous thyroid parenchyma. Also, in
follicular cysts were observed in the enlarged gland with no echo
structures of different sizes and decreased echogenicity as a local or
general. In nodular hyperplasia, increase echogenicity and
heterogeneous parenchymal were seen. These findings suggest the
use of sonography and pathology as a screening test in the diagnosis
of complications of thyroid disorders.
Abstract: Transpedicular screw fixation in spinal fractures,
degenerative changes, or deformities is a well-established procedure.
However, important rate of fixation failure due to screw bending,
loosening, or pullout are still reported particularly in weak bone stock
in osteoporosis. To overcome the problem, mechanism of failure has
to be fully investigated in vitro. Post-mortem human subjects are less
accessible and animal cadavers comprise limitations due to different
geometry and mechanical properties. Therefore, the development of a
synthetic model mimicking the realistic human vertebra is highly
demanded. A bone surrogate, composed of Polyurethane (PU) foam
analogous to cancellous bone porous structure, was tested for 3
different densities in this study. The mechanical properties were
investigated under uniaxial compression test by minimizing the end
artifacts on specimens. The results indicated that PU foam of 0.32
g.cm-3 density has comparable mechanical properties to human
cancellous bone in terms of young-s modulus and yield strength.
Therefore, the obtained information can be considered as primary
step for developing a realistic cancellous bone of human vertebral
body. Further evaluations are also recommended for other density
groups.