Abstract: Highly pathogenic avian influenza (HPAI) H5N1 viruses have created demand for a cost-effective vaccine to prevent a pandemic of the disease. Here, we report that Trichoplusia ni (T. ni) larvae can act as a cost-effective bioreactor to produce recombinant HA5 (rH5HA) proteins as an potential effective vaccine for chickens. To facilitate the recombinant virus identification, virus titer determination and access the infected larvae, we employed the internal ribosome entry site (IRES) derived from Perina nuda virus (PnV, belongs to insect picorna like Iflavirus genus) to construct a bi-cistronic baculovirus expression vector that can express the rH5HA protein and enhanced green fluorescent protein (EGFP) simultaneously. Western blot analysis revealed that the 70 kDa rH5HA protein and partially cleaved products (40 kDa H5HA1) were generated in T. ni larvae infected with recombinant baculovirus carrying the H5HA gene. These data suggest that the baculovirus-larvae recombinant protein expression system could be a cost-effective platform for H5N1 vaccine production.
Abstract: Due to short product life cycles, increasing variety of
products and short cycles of leap innovations manufacturing
companies have to increase the flexibility of factory structures.
Flexibility of factory structures is based on defined factory planning
processes in which product, process and resource data of various
partial domains have to be considered. Thus factory planning
processes can be characterized as iterative, interdisciplinary and
participative processes [1]. To support interdisciplinary and
participative character of planning processes, a federative factory
data management (FFDM) as a holistic solution will be described.
FFDM is already implemented in form of a prototype. The interim
results of the development of FFDM will be shown in this paper. The
principles are the extracting of product, process and resource data
from documents of various partial domains providing as web services
on a server. The described data can be requested by the factory
planner by using a FFDM-browser.
Abstract: A new decomposition form is introduced in this report
to establish a criterion for the bi-partite separability of Bell diagonal
states. A such criterion takes a quadratic inequality of the coefficients
of a given Bell diagonal states and can be derived via a simple
algorithmic calculation of its invariants. In addition, the criterion can
be extended to a quantum system of higher dimension.
Abstract: Developing a university course schedule is
difficult. This is due to the limitations in the resources
available. The process is made even harder with different
faculties or departments having different ways of stating their
schedule requirements. The person in charge of taking the
schedule requirements and turning them into a proper course
schedule is not only burden with the task of allocating the
appropriate classes and time to lecturers and students, they
also need to understand the schedule requirements. Therefore
a scheduling support system named SATA is developed to
assist ICRESS in the course scheduling process. SATA has
been put to use for several semesters and the results have been
encouraging. It won a bronze medal in the 2008 Invention,
Innovation and Design competition (IID-08) and has been
submitted to be patented in October 2008
Abstract: Functional gastrointestinal disorders (FGID) affect millions of people spread all age regardless of race and sex. Emotional stress and obesity have been associated with increased reporting of gastrointestinal (GI) symptoms, but the relationship between FGID and risk factors (emotional stress or obesity) is unclear. Our aim was to assess the changes of the mechanical characteristics on the gastrointestinal tracts of the mentally fatigued obese and normal rat models. Finally, using the physical characteristics with micro-indentation test, we made a close investigation into the relation between FGID and risk factors quantitatively.
Abstract: Training with Functional Electrical Stimulation (FES)
has both physiological and psychological benefits for spinal cord
injured subjects. Commonly used methods for quantification of
spasticity have shown controversial reliability. In this study we
propose a method for quick determination of spasticity in spinal cord
injured subjects on a cycling and measurement system. 23 patients
did training sessions on an instrumented mobile FES cycle three
times a week over two months as part of their clinical rehabilitation
program. Spasticity (MAS) and the legs resistance to the pedaling
motion were assessed before and after the FES training and
measurements were done on the subjects ability to pedal with our
without motor assistance. Measurements with test persons with
incomplete spastic paraplegia have shown that spasticity is decreased
after a 30 min cycling training with functional electrical stimulation
(FES).
Abstract: Pepper spray use has gained momentum since 1992
and although the active ingredient is readily available, it is considered
a weapon with restricted use in many regions, including The
Bahamas. In light of controversy in the literature regarding the
severity of presenting respiration complaints among individuals postacute
exposure of pepper spray inhalation, this descriptive case series
study was conducted to assess the respiratory status of persons
evaluated during a mass casualty in The Bahamas. Parameters noted
were patients- demographics and respiration severity determined via
clinical examination findings, disposition and follow-up review of the
20 persons. Their most common complaint was difficulty breathing
post exposure. Two required admission and stayed for
Abstract: Component-Based software engineering provides an
opportunity for better quality and increased productivity in software
development by using reusable software components [10]. One of the
most critical aspects of the quality of a software system is its
performance. The systematic application of software performance
engineering techniques throughout the development process can help
to identify design alternatives that preserve desirable qualities such
as extensibility and reusability while meeting performance objectives
[1]. In the present scenario, software engineering methodologies
strongly focus on the functionality of the system, while applying a
“fix- it-later" approach to software performance aspects [3]. As a
result, lengthy fine-tunings, expensive extra hard ware, or even
redesigns are necessary for the system to meet the performance
requirements. In this paper, we propose design based,
implementation independent, performance prediction approach to
reduce the overhead associated in the later phases while developing a
performance guaranteed software product with the help of Unified
Modeling Language (UML).
Abstract: This paper describes dynamic analysis using proposed
fast finite element method for a shock absorbing structure including a
sponge. The structure is supported by nonlinear concentrated springs.
The restoring force of the spring has cubic nonlinearity and linear
hysteresis damping. To calculate damping properties for the structures
including elastic body and porous body, displacement vectors as
common unknown variable are solved under coupled condition. Under
small amplitude, we apply asymptotic method to complex eigenvalue
problem of this system to obtain modal parameters. And then
expressions of modal loss factor are derived approximately. This
approach was proposed by one of the authors previously. We call this
method as Modal Strain and Kinetic Energy Method (MSKE method).
Further, using the modal loss factors, the discretized equations in
physical coordinate are transformed into the nonlinear ordinary
coupled equations using normal coordinate corresponding to linear
natural modes. This transformation yields computation efficiency. As
a numerical example of a shock absorbing structures, we adopt double
skins with a sponge. The double skins are supported by nonlinear
concentrated springs. We clarify influences of amplitude of the input
force on nonlinear and chaotic responses.
Abstract: Sign language is used by the deaf and hard of hearing people for communication. Automatic sign language recognition is a challenging research area since sign language often is the only way of communication for the deaf people. Sign language includes different components of visual actions made by the signer using the hands, the face, and the torso, to convey his/her meaning. To use different aspects of signs, we combine the different groups of features which have been extracted from the image frames recorded directly by a stationary camera. We combine the features in two levels by employing three techniques. At the feature level, an early feature combination can be performed by concatenating and weighting different feature groups, or by concatenating feature groups over time and using LDA to choose the most discriminant elements. At the model level, a late fusion of differently trained models can be carried out by a log-linear model combination. In this paper, we investigate these three combination techniques in an automatic sign language recognition system and show that the recognition rate can be significantly improved.
Abstract: The objective of this work is to produce heterotrophic
microalgal lipid in flask-batch fermentation. Chlorella sp. KKU-S2
supported maximum values of 0.374 g/L/d, 0.478 g lipid/g cells, and
0.112 g/L/d for volumetric lipid production rate, and specific yield of
lipid, and specific rate of lipid production, respectively when culture
was performed on BG-11 medium supplemented with 50g/L glucose.
Among the carbon sources tested, maximum cell yield coefficient
(YX/S, g/L), maximum specific yield of lipid (YP/X, g lipid/g cells) and
volumetric lipid production rate (QP, g/L/d) were found of 0.728,
0.237, and 0.619, respectively, using sugarcane molasses as carbon
source. The main components of fatty acid from extracted lipid were
palmitic acid, stearic acid, oleic acid and linoleic acid which similar
to vegetable oils and suitable for biodiesel production.
Abstract: The usual method of river flow diversion involves construction of tunnels and cofferdams. Given the fact that the cost of diversion works could be as high as 10-20% of the total dam construction cost, due attention should be paid to optimum design of the diversion works. The cost of diversion works depends, on factors, such as: the tunnel dimensions and the intended tunneling support measures during and after excavation; quality and characterizes of the rock through which the tunnel should be excavated; the dimensions of the upstream (and downstream) cofferdams; and the magnitude of river flood the system is designed to divert. In this paper by use of the cost of unit prices for tunnel excavation, tunnel lining, tunnel support (rock bolt + shotcrete) and cofferdam fill the cost function was determined. The function is then minimized by the aid of PSO Algorithm (particle swarm optimization). It is found that the optimum diameter and the total diversion cost are directly related to the river flood discharge (Q). It has also shown that in addition to optimum diameter design discharge (Q), river length, tunnel length, is mainly a function of the ratios (not the absolute values) of the unit prices and does not depend on the overall price levels in the respective country. The results of optimization use in some of the case study lead us to significant changes in the cost.
Abstract: Study fluid flow and heat transfer characteristics of
microchannel in a primary Cross-corrugated(CC) surface recuperators
with corrugations and without corrugations, using CFD method. The
pitch-over-height ratios P/H of Cross-corrugated (CC) surface is from
1.5 to 4.0, included angles β=75º. The study was performed using CFD
software FLUENT to create unit model and simulate fluid
temperature, velocity, heat transfer coefficient and other parameters.
The results from these simulations were compared to experimental
data. It is concluded that, when the Reynolds number is constant, if
increase P/H, j/f will decrease, also the decreasing trend will become
weak. Under the condition of P/H=2.2, if increase the inlet velocity j/f
will decrease; in addition, the heat transfer performance in surface
with corrugation will increase 10% compared to that without
corrugation. The study results can provide the basis to optimize the
design, select the type of heat transfer surface, the scale structure, and
heat-transfer surface arrangement for recuperators.
Abstract: The report focuses on such an important indicator of the nature and direction of development of ethnic and cultural processes in the Republic of Kazakhstan, as ethno linguistic situation. It is shown that, in essence, on the one hand, expresses the degree of the actual propagation and the level of use of the languages of the various ethnic communities. On the other hand, reflects the important patterns, trends and prospects of ethno-cultural and ethnodemographic processes in the Republic. It is important to note that the ethno linguistic situation in different regions of Kazakhstan, due to its more dynamic and much more difficult to demonstrate a much greater variety of options when compared with the ethnic situation in the country. For the two major ethnic groups of the republic – Kazakh and Russian language ethno differentiating retains its value, while for the other ethnic groups observed decline in the importance of this indicator. As you know, the language of international communication in the country is Russian. As the censuses of population, the Russian language in many areas of Northern, Central and Eastern Kazakhstan becomes a means of ethno linguistic development for most of the non-Russian population. This is most clearly illustrated by the Germans, and the Slavic ethnic groups. In this case, the Russian language is not just a means of international communication for a number of ethnic groups, and ethnic groups, it becomes a factor of ethnic self-expression. The value of the Kazakh language as their mother tongue for the other groups of the population is small. More clearly it can be traced only to the Turkic-speaking population of the republic – Uzbeks, Uighurs, Tatars, Turks, etc. The state Kazakh language is a means of international communication in the Western and Southern Kazakhstan, with a predominance of the Kazakh population. The report shows that the most important factor in the development of ethno-linguistic and ethno-cultural processes is bilingualism. Comparative analysis of materials census shows, first, on the increase of the proportion of bilingual population among Kazakhs and Russian, and second, to reduce the proportion of bilingual population of other ethnic groups living in Kazakhstan, and third, a higher proportion bilingual population among residents than rural residents, regardless of their ethnicity. Bilingualism is mainly of a "national Kazakh", "national Russian" or "Kazakh-national" or "Russian-national" character. The President N.A. Nazarbayev said that the Kazakh language is the most important factor in the consolidation of the people of Kazakhstan. He therefore called on government and other state and local representative bodies fully develop the state language, to create all the necessary organizational, material and technical conditions for free and open learning the state language by all citizens of the Republic of Kazakhstan.
Abstract: The paper presents the design concept of a unitselection
text-to-speech synthesis system for the Slovenian language.
Due to its modular and upgradable architecture, the system can be
used in a variety of speech user interface applications, ranging from
server carrier-grade voice portal applications, desktop user interfaces
to specialized embedded devices.
Since memory and processing power requirements are important
factors for a possible implementation in embedded devices, lexica
and speech corpora need to be reduced. We describe a simple and
efficient implementation of a greedy subset selection algorithm that
extracts a compact subset of high coverage text sentences. The
experiment on a reference text corpus showed that the subset
selection algorithm produced a compact sentence subset with a small
redundancy.
The adequacy of the spoken output was evaluated by several
subjective tests as they are recommended by the International
Telecommunication Union ITU.
Abstract: The operating control parameters of injection
flushing type of electrical discharge machining process on stainless
steel 304 workpiece using copper tools are being optimized
according to its individual machining characteristic i.e. Electrode
Wear Ratio (EWR). Higher EWR would give bad dimensional
precision for the EDM machined workpiece because of high
electrode wear. Hence, the quality characteristic for EWR is set to
lower-the-better to achieve the optimum dimensional precision for
the machined workpiece. Taguchi method has been used for the
construction, layout and analysis of the experiment for EWR
machining characteristic. The use of Taguchi method in the
experiment saves a lot of time and cost of preparing and machining
the experiment samples. Therefore, an L18 Orthogonal array
which was the fundamental component in the statistical design of
experiments has been used to plan the experiments and Analysis of
Variance (ANOVA) is used to determine the optimum machining
parameters for this machining characteristic. The control
parameters selected for this optimization experiments are polarity,
pulse on duration, discharge current, discharge voltage, machining
depth, machining diameter and dielectric liquid pressure. The
result had shown that negative polarity machining parameter
setting will decreases EWR.
Abstract: In the present work, a comparative study on the
microstructure and mechanical properties of as cast, cast aged and
forged aged A356 alloy has been investigated. The study reveals that
mechanical properties of A356 alloy are highly influenced by melt
treatment and solid state processing. Cast aged alloys achieve highest
strength and hardness compared to as cast and forge aged ones. Ones
treated with combined addition of grain refiners and modifiers
achieve maximum strength and hardness. Cast aged A356 alloy
possesses higher wear resistance compared to as cast and forge aged
ones. Forging improves both strength and ductility of alloys over as
cast ones. However, the improvement in ductility is perceptible only
for properly grain refined and modified alloys. Ones refined with
0.65% Al-3Ti shows highest improvement in ductility while ones
treated with 0.20% Al-10Sr exhibits less improvement in ductility.
Abstract: In recent times there has been a growing interest in the
development of quasi-two-dimensional niobium pentoxide (Nb2O5)
as a semiconductor for the potential electronic applications such as
capacitors, filtration, dye-sensitised solar cells and gas sensing
platforms. Therefore once the purpose is established, Nb2O5 can be
prepared in a number of nano- and sub-micron-structural
morphologies that include rods, wires, belts and tubes. In this study
films of Nb2O5 were prepared on gold plated silicon substrate using
spin-coating technique and subsequently by mechanical exfoliation.
The reason this method was employed was to achieve layers of less
than 15nm in thickness. The sintering temperature of the specimen
was 800oC. The morphology and structural characteristics of the
films were analyzed by Atomic Force Microscopy (AFM), Raman
Spectroscopy, X-ray Photoelectron Spectroscopy (XPS).
Abstract: Climate change is a cumulative change in weather
patterns over a period of time. Trend analysis using non-parametric
Mann-Kendall test may help to determine the existence and
magnitude of any statistically significant trend in the climatic data.
Another index called Sen slope may be used to quantify the
magnitude of such trends. A toolbar extension to ESRI ArcGIS
named Arc Trends has been developed in this study for performing
the above mentioned tasks. To study the temporal trend of
meteorological parameters, 32 years (1971-2002) monthly
meteorological data were collected for 133 selected stations over
different agro-ecological regions of India. Both the maximum and
minimum temperatures were found to be rising. A significant
increasing trend in the relative humidity and a consistent significant
decreasing trend in the wind speed all over the country were found.
However, a general increase in rainfall was not found in recent years.
Abstract: This study examines regional convergence in per capita personal income in the US and Canada. We find that the disparity in real per capita income levels across US states (Canadian provinces) has declined, but income levels are not identical. Income levels become more aligned once costs of living are accounted for in relative per capita income series. US states (Canadian provinces) converge at an annual rate of between 1.3% and 2.04% (between 2.15% and 2.37%). A pattern of σ and β-convergence in per capita personal income across regions evident over the entire sample period, is reversed over 1979-1989 (1976-1990) period. The reversal may be due to sectoral or region-specific shocks that have highly persistent effects. The latter explanation might be true for half of the US and most of Canada.