Effect of Genotype, Explant Type and Growth Regulators on The Accumulation of Flavonoides of (Silybum marianum L.) in In vitro Culture

The extract of milk thistle contains a mix of flavonolignans termed silymarine.. In order to analysis influence of growth regulators, genotype, explant and subculture on the accumulation of flavonolignans, a study was carried out by using two genotype (Budakalszi and Noor abad moghan cultivars), cotyledon and hypocotyle explants, solid media of MS supplemented by different combinations of two growth regulators; Kinetin (0.1, 1 mg/l) and 2,4-D (1, 2 mg/l). Seeds of the plant were germinated in MS media whitout growth regulators in growth chamber at 26°C and darkness condition. In order to callus induction, the culture media was supplemented whit different concentrations of 2,4-D and kinetin. Calli obtained from explants were sub-cultured four times into the fresh media of the first experiment. flavonoides was extracted from calli in four subcultures. The flavonoid components were determined by high- performance liquid choromatography (HPLC) and separated into Taxifolin, Silydianin+Silychristin, Silybin A+B and Isosilybin A+B. Results showed that with increasing callus age, increased accumulation of silybin A+B, but reduced Isosilybin A+B content. Highest accumulation of Taxifolin was observed at first calli. Calli produced from cotyledon explant of Budakalszi cultivar were superior for Silybin A+B, where calli from hypocotyl explant produced higher amount of Taxifolin and Silydianin+Silychristin. The best cultivar for Silymarin production in this study was Budakalszi cultivar. High amount of SBN A+B and TXF were obtained from hypocotil explant.

Job Stressors and Coping Mechanisms among Emergency Department Nurses in the Armed Force Hospitals of Taiwan

Nurses in an Armed Force Hospital (AFH) expose to stronger stress than those in a civil hospital, especially in an emergency department (ED). Ironically, stresses of these nurses received few if any attention in academic research in the past. This study collects 227 samples from the emergency departments of four armed force hospitals in central and southern Taiwan. The research indicates that the top five stressors are a massive casualty event, delayed physician support, overloads of routine work, overloads of assignments, and annoying paper work. Excessive work loading was found to be the primary source of stress. Nurses who were perceived to have greater stress levels were more inclined to deploy emotion-oriented approaches and more likely to seek job rotations. Professional stressors and problem-oriented approaches were positively correlated. Unlike other local studies, this study concludes that the excessive work-loading is more stressful in an AFH.

In vivo Introduced Extracellular Ubiquitin Regulates Intracellular Processes

Extracellular ubiquitin in vivo effect on regenerative liver cells and liver histoarchitectonics has been studied. Experiments were performed on mature female white rats. Partial hepatectomy was made using the modified method of Higgins and Anderson. Standard histopathological assessment of liver tissue was used. Proliferative activity of hepatocytes was analyzed by colchicine mitotic index and immunohistochemical staining on ki67. We have found that regardless of number of injections and dose of extracellular ubiquitin liver histology has not been changed, so at tissue level no effect was observed. In vivo double injection of ubiquitin significantly decreases the mitotic activity at 32 hour point after partial hepatectomy. Thus, we can conclude that in vivo injected extracellular ubiquitin inhibits proliferative activity of hepatocytes in partially hepatectomyzed rats.

Modelling of a Stress-Strain State of Screws of Transpedicular Spine Fixation System

For maintenance of a spine stability during the postoperative period a transpedicular fixing of its elements is often used. Usually the transpedicular systems are formed of rods which as a result form a design of the frame type, fastening by screws to vertebras. Such design should be rigid and perceive loadings operating from the spine without essential deformations. From the perfection point of view of known designs their stress whole, and each of elements, in particular is of interest. In this study the modeling of the transpedicular screw is performed and estimation of its deformations taking into account interaction with a vertebra body having variable structure is made.

A Framework for Data Mining Based Multi-Agent: An Application to Spatial Data

Data mining is an extraordinarily demanding field referring to extraction of implicit knowledge and relationships, which are not explicitly stored in databases. A wide variety of methods of data mining have been introduced (classification, characterization, generalization...). Each one of these methods includes more than algorithm. A system of data mining implies different user categories,, which mean that the user-s behavior must be a component of the system. The problem at this level is to know which algorithm of which method to employ for an exploratory end, which one for a decisional end, and how can they collaborate and communicate. Agent paradigm presents a new way of conception and realizing of data mining system. The purpose is to combine different algorithms of data mining to prepare elements for decision-makers, benefiting from the possibilities offered by the multi-agent systems. In this paper the agent framework for data mining is introduced, and its overall architecture and functionality are presented. The validation is made on spatial data. Principal results will be presented.

Client Server System for e-Services Access Using Mobile Communications Networks

The client server systems using mobile communications networks for data transmission became very attractive for many economic agents, in the purpose of promoting and offering electronic services to their clients. E-services are suitable for business developing and financial benefits increasing. The products or services can be efficiently delivered to a large number of clients, using mobile Internet access technologies. The clients can have access to e-services, anywhere and anytime, with the support of 3G, GPRS, WLAN, etc., channels bandwidth, data services and protocols. Based on the mobile communications networks evolution and development, a convergence of technological and financial interests of mobile operators, software developers, mobile terminals producers and e-content providers is established. These will lead to a high level of integration of IT&C resources and will facilitate the value added services delivery through the mobile communications networks. In this paper it is presented a client server system, for e-services access, with Smartphones and PDA-s mobile software applications, installed on Symbian and Windows Mobile operating systems.

Implementation of Adder-Subtracter Design with VerilogHDL

According to the density of the chips, designers are trying to put so any facilities of computational and storage on single chips. Along with the complexity of computational and storage circuits, the designing, testing and debugging become more and more complex and expensive. So, hardware design will be built by using very high speed hardware description language, which is more efficient and cost effective. This paper will focus on the implementation of 32-bit ALU design based on Verilog hardware description language. Adder and subtracter operate correctly on both unsigned and positive numbers. In ALU, addition takes most of the time if it uses the ripple-carry adder. The general strategy for designing fast adders is to reduce the time required to form carry signals. Adders that use this principle are called carry look- ahead adder. The carry look-ahead adder is to be designed with combination of 4-bit adders. The syntax of Verilog HDL is similar to the C programming language. This paper proposes a unified approach to ALU design in which both simulation and formal verification can co-exist.

Analysis and Research of Two-Level Scheduling Profile for Open Real-Time System

In an open real-time system environment, the coexistence of different kinds of real-time and non real-time applications makes the system scheduling mechanism face new requirements and challenges. One two-level scheduling scheme of the open real-time systems is introduced, and points out that hard and soft real-time applications are scheduled non-distinctively as the same type real-time applications, the Quality of Service (QoS) cannot be guaranteed. It has two flaws: The first, it can not differentiate scheduling priorities of hard and soft real-time applications, that is to say, it neglects characteristic differences between hard real-time applications and soft ones, so it does not suit a more complex real-time environment. The second, the worst case execution time of soft real-time applications cannot be predicted exactly, so it is not worth while to cost much spending in order to assure all soft real-time applications not to miss their deadlines, and doing that may cause resource wasting. In order to solve this problem, a novel two-level real-time scheduling mechanism (including scheduling profile and scheduling algorithm) which adds the process of dealing with soft real-time applications is proposed. Finally, we verify real-time scheduling mechanism from two aspects of theory and experiment. The results indicate that our scheduling mechanism can achieve the following objectives. (1) It can reflect the difference of priority when scheduling hard and soft real-time applications. (2) It can ensure schedulability of hard real-time applications, that is, their rate of missing deadline is 0. (3) The overall rate of missing deadline of soft real-time applications can be less than 1. (4) The deadline of a non-real-time application is not set, whereas the scheduling algorithm that server 0 S uses can avoid the “starvation" of jobs and increase QOS. By doing that, our scheduling mechanism is more compatible with different types of applications and it will be applied more widely.

A Distributed Group Mutual Exclusion Algorithm for Soft Real Time Systems

The group mutual exclusion (GME) problem is an interesting generalization of the mutual exclusion problem. Several solutions of the GME problem have been proposed for message passing distributed systems. However, none of these solutions is suitable for real time distributed systems. In this paper, we propose a token-based distributed algorithms for the GME problem in soft real time distributed systems. The algorithm uses the concepts of priority queue, dynamic request set and the process state. The algorithm uses first come first serve approach in selecting the next session type between the same priority levels and satisfies the concurrent occupancy property. The algorithm allows all n processors to be inside their CS provided they request for the same session. The performance analysis and correctness proof of the algorithm has also been included in the paper.

A Model Predictive Control and Time Series Forecasting Framework for Supply Chain Management

Model Predictive Control has been previously applied to supply chain problems with promising results; however hitherto proposed systems possessed no information on future demand. A forecasting methodology will surely promote the efficiency of control actions by providing insight on the future. A complete supply chain management framework that is based on Model Predictive Control (MPC) and Time Series Forecasting will be presented in this paper. The proposed framework will be tested on industrial data in order to assess the efficiency of the method and the impact of forecast accuracy on overall control performance of the supply chain. To this end, forecasting methodologies with different characteristics will be implemented on test data to generate forecasts that will serve as input to the Model Predictive Control module.

Evaluation of Optimum Performance of Lateral Intakes

In designing river intakes and diversion structures, it is paramount that the sediments entering the intake are minimized or, if possible, completely separated. Due to high water velocity, sediments can significantly damage hydraulic structures especially when mechanical equipment like pumps and turbines are used. This subsequently results in wasting water, electricity and further costs. Therefore, it is prudent to investigate and analyze the performance of lateral intakes affected by sediment control structures. Laboratory experiments, despite their vast potential and benefits, can face certain limitations and challenges. Some of these include: limitations in equipment and facilities, space constraints, equipment errors including lack of adequate precision or mal-operation, and finally, human error. Research has shown that in order to achieve the ultimate goal of intake structure design – which is to design longlasting and proficient structures – the best combination of sediment control structures (such as sill and submerged vanes) along with parameters that increase their performance (such as diversion angle and location) should be determined. Cost, difficulty of execution and environmental impacts should also be included in evaluating the optimal design. This solution can then be applied to similar problems in the future. Subsequently, the model used to arrive at the optimal design requires high level of accuracy and precision in order to avoid improper design and execution of projects. Process of creating and executing the design should be as comprehensive and applicable as possible. Therefore, it is important that influential parameters and vital criteria is fully understood and applied at all stages of choosing the optimal design. In this article, influential parameters on optimal performance of the intake, advantages and disadvantages, and efficiency of a given design are studied. Then, a multi-criterion decision matrix is utilized to choose the optimal model that can be used to determine the proper parameters in constructing the intake.

New Approach in Diagnostics Method for Milling Process using Envelope Analysis

This paper proposes a method to vibration analysis in order to on-line monitoring and predictive maintenance during the milling process. Adapting envelope method to diagnostics and the analysis for milling tool materials is an important contribution to the qualitative and quantitative characterization of milling capacity and a step by modeling the three-dimensional cutting process. An experimental protocol was designed and developed for the acquisition, processing and analyzing three-dimensional signal. The vibration envelope analysis is proposed to detect the cutting capacity of the tool with the optimization application of cutting parameters. The research is focused on Hilbert transform optimization to evaluate the dynamic behavior of the machine/ tool/workpiece.

Impulse Noise Reduction in Brain Magnetic Resonance Imaging Using Fuzzy Filters

Noise contamination in a magnetic resonance (MR) image could occur during acquisition, storage, and transmission in which effective filtering is required to avoid repeating the MR procedure. In this paper, an iterative asymmetrical triangle fuzzy filter with moving average center (ATMAVi filter) is used to reduce different levels of salt and pepper noise in a brain MR image. Besides visual inspection on filtered images, the mean squared error (MSE) is used as an objective measurement. When compared with the median filter, simulation results indicate that the ATMAVi filter is effective especially for filtering a higher level noise (such as noise density = 0.45) using a smaller window size (such as 3x3) when operated iteratively or using a larger window size (such as 5x5) when operated non-iteratively.

A Study on the Leadership Behavior, Safety Culture, and Safety Performance of the Healthcare Industry

Object: Review recent publications of patient safety culture to investigate the relationship between leadership behavior, safety culture, and safety performance in the healthcare industry. Method: This study is a cross-sectional study, 350 questionnaires were mailed to hospital workers with 195 valid responses obtained, and a 55.7% valid response rate. Confirmatory factor analysis (CFA) was carried out to test the factor structure and determine if the composite reliability was significant with a factor loading of >0.5, resulting in an acceptable model fit. Results: Through the analysis of One-way ANOVA, the results showed that physicians significantly have more negative patient safety culture perceptions and safety performance perceptions than non- physicians. Conclusions: The path analysis results show that leadership behavior affects safety culture and safety performance in the health care industry. Safety performance was affected and improved with contingency leadership and a positive patient safety organization culture. The study suggests improving safety performance by providing a well-managed system that includes: consideration of leadership, hospital worker training courses, and a solid safety reporting system.

Social Marketing and Nonprofit Organizations

Today the social marketing was constituted as a tool of significant value in what he refers to the promotion of changes of behaviors, attitudes end practices. With the objective of analyzing the benefits that the social marketing can bring for the organizations that use it the research was of the exploratory and descriptive. In the present study the comparative method was used, through a qualitative approach, to analyze the activities developed by three institutions: the Recovery Center Rosa de Saron, the House of Recovery for addicts and Teen Challenge Institute Children's Cancer of the Wasteland (ICIA), kindred of pointing out the benefits of the social marketing in organizations that don-t seek the profit.

Software Industrialization in Systems Integration

Today-s economy is in a permanent change, causing merger and acquisitions and co operations between enterprises. As a consequence, process adaptations and realignments result in systems integration and software development projects. Processes and procedures to execute such projects are still reliant on craftsman-ship of highly skilled workers. A generally accepted, industrialized production, characterized by high efficiency and quality, seems inevitable. In spite of this, current concepts of software industrialization are aimed at traditional software engineering and do not consider the characteristics of systems integration. The present work points out these particularities and discusses the applicability of existing industrial concepts in the systems integration domain. Consequently it defines further areas of research necessary to bring the field of systems integration closer to an industrialized production, allowing a higher efficiency, quality and return on investment.

Testing Visual Abilities of Machines - Visual Intelligence Tests

Intelligence tests are series of tasks designed to measure the capacity to make abstractions, to learn, and to deal with novel situations. Testing of the visual abilities of the shape understanding system (SUS) is performed based on the visual intelligence tests. In this paper the progressive matrices tests are formulated as tasks given to SUS. These tests require good visual problem solving abilities of the human subject. SUS solves these tests by performing complex visual reasoning transforming the visual forms (tests) into the string forms. The experiment proved that the proposed method, which is part of the SUS visual understanding abilities, can solve a test that is very difficult for human subject.

Hydrated Magnesium Borate Synthesis from MgCl2.6H2O at 80oC by Hydrothermal Method

Borate minerals have attracted considerable attention in the past years due to their structural chemistry and mechanical properties in several industries. Recently, increasing attention has been paid to the use of; synthetically produced magnesium borates as catalysts reinforcing material for plastics, the conversion of hydrocarbons, electro-conductive treating agent, anti-wear and anti-corrosion materials. Magnesium borates can be synthesized by several methods such as; hydrothermal and solid-state (thermal) processes. In this study the hydrothermal production method was applied at the modest temperature of 80C along with convenient crystal growth. Using MgCl2.6H2O, H3BO3, and NaOH as starting materials, 30, 60, 120, 240 minutes of reaction times were studied. After all, the crystal structure and the morphology of the products were examined by X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FT-IR). As a result the forms of Admontite and Mcallisterite minerals were synthesized.

Comprehensive Studies on Mechanical Stress Analysis of Functionally Graded Plates

Stress analysis of functionally graded composite plates composed of ceramic, functionally graded material and metal layers is investigated using 3-D finite element method. In FGM layer, material properties are assumed to be varied continuously in the thickness direction according to a simple power law distribution in terms of the volume fraction of a ceramic and metal. The 3-D finite element model is adopted by using an 18-node solid element to analyze more accurately the variation of material properties in the thickness direction. Numerical results are compared for three types of materials. In the analysis, the tensile and the compressive stresses are summarized for various FGM thickness ratios, volume fraction distributions, geometric parameters and mechanical loads.

Physical and Electrical Characterization of ZnO Thin Films Prepared by Sol-Gel Method

In this paper, Zinc Oxide (ZnO) thin films are deposited on glass substrate by sol-gel method. The ZnO thin films with well defined orientation were acquired by spin coating of zinc acetate dehydrate monoethanolamine (MEA), de-ionized water and isopropanol alcohol. These films were pre-heated at 275°C for 10 min and then annealed at 350°C, 450°C and 550°C for 80 min. The effect of annealing temperature and different thickness on structure and surface morphology of the thin films were verified by Atomic Force Microscopy (AFM). It was found that there was a significant effect of annealing temperature on the structural parameters of the films such as roughness exponent, fractal dimension and interface width. Thin films also were characterizied by X-ray Diffractometery (XRD) method. XRD analysis revealed that the annealed ZnO thin films consist of single phase ZnO with wurtzite structure and show the c-axis grain orientation. Increasing annealing temperature increased the crystallite size and the c-axis orientation of the film after 450°C. Also In this study, ZnO thin films in different thickness have been prepared by sol-gel method on the glass substrate at room temperature. The thicknesses of films are 100, 150 and 250 nm. Using fractal analysis, morphological characteristics of surface films thickness in amorphous state were investigated. The results show that with increasing thickness, surface roughness (RMS) and lateral correlation length (ξ) are decreased. Also, the roughness exponent (α) and growth exponent (β) were determined to be 0.74±0.02 and 0.11±0.02, respectively.