Abstract: A new strategy for oriented immobilization of proteins was proposed. The strategy contains two steps. The first step is to search for a docking site away from the active site on the protein surface. The second step is trying to find a ligand that is able to grasp the targeted site of the protein. To avoid ligand binding to the active site of protein, the targeted docking site is selected to own opposite charges to those near the active site. To enhance the ligand-protein binding, both hydrophobic and electrostatic interactions need to be included. The targeted docking site should therefore contain hydrophobic amino acids. The ligand is then selected through the help of molecular docking simulations. The enzyme α-amylase derived from Aspergillus oryzae (TAKA) was taken as an example for oriented immobilization. The active site of TAKA is surrounded by negatively charged amino acids. All the possible hydrophobic sites on the surface of TAKA were evaluated by the free energy estimation through benzene docking. A hydrophobic site on the opposite side of TAKA-s active site was found to be positive in net charges. A possible ligand, 3,3-,4,4- – Biphenyltetra- carboxylic acid (BPTA), was found to catch TAKA by the designated docking site. Then, the BPTA molecules were grafted onto silica gels and measured the affinity of TAKA adsorption and the specific activity of thereby immobilized enzymes. It was found that TAKA had a dissociation constant as low as 7.0×10-6 M toward the ligand BPTA on silica gel. The increase in ionic strength has little effect on the adsorption of TAKA, which indicated the existence of hydrophobic interaction between ligands and proteins. The specific activity of the immobilized TAKA was compared with the randomly adsorbed TAKA on primary amine containing silica gel. It was found that the orderly immobilized TAKA owns a specific activity twice as high as the one randomly adsorbed by ionic interaction.
Abstract: Mobile Ad hoc Network (MANET) is a wireless ad hoc self-configuring network of mobile routers (and associated hosts) connected by wireless links, the union of which forms an arbitrary topology, cause of the random mobility of the nodes. In this paper, an attempt has been made to compare these three protocols DSDV, AODV and DSR on the performance basis under different traffic protocols namely CBR and TCP in a large network. The simulation tool is NS2, the scenarios are made to see the effect of pause times. The results presented in this paper clearly indicate that the different protocols behave differently under different pause times. Also, the results show the main characteristics of different traffic protocols operating on MANETs and thus select the best protocol on each scenario.
Abstract: The effect of seed inoculation by VA- mycorrhiza and
different levels of phosphorus fertilizer on growth and yield of
sunflower (Azargol cultivar) was studied in experiment farm of
Islamic Azad University, Karaj Branch during 2008 growing season.
The experiment treatments were arranged in factorial based on a
complete randomized block design with three replications. Four
phosphorus fertilizer levels of 25%, 50% 75% and 100% P
recommended with two levels of Mycorrhiza: with and without
Mycorrhiza (control) were assigned in a factorial combination.
Results showed that head diameter, number of seeds in head, seed
yield and oil yield were significantly higher in inoculated plants than
in non-inoculated plants. Head diameter, number of seeds in head,
1000 seeds weight, biological yield, seed yield and oil yield increased
with increasing P level above 75% P recommended in non-inoculated
plants, whereas no significant difference was observed between 75%
and 100% P recommended. The positive effect of mycorrhizal
inoculation decreased with increasing P levels due to decreased
percent root colonization at higher P levels. According to the results
of this experiment, application of mycorrhiza in present of 50% P
recommended had an appropriate performance and could increase
seed yield and oil production to an acceptable level, so it could be
considered as a suitable substitute for chemical phosphorus fertilizer
in organic agricultural systems.
Abstract: Recently the usefulness of Concept Abduction, a novel non-monotonic inference service for Description Logics (DLs), has been argued in the context of ontology-based applications such as semantic matchmaking and resource retrieval. Based on tableau calculus, a method has been proposed to realize this reasoning task in ALN, a description logic that supports simple cardinality restrictions as well as other basic constructors. However, in many ontology-based systems, the representation of ontology would require expressive formalisms for capturing domain-specific constraints, this language is not sufficient. In order to increase the applicability of the abductive reasoning method in such contexts, we would like to present in the scope of this paper an extension of the tableaux-based algorithm for dealing with concepts represented inALCQ, the description logic that extends ALN with full concept negation and quantified number restrictions.
Abstract: Fuzzy logic can be used when knowledge is
incomplete or when ambiguity of data exists. The purpose of
this paper is to propose a proactive fuzzy set- based model for
reacting to the risk inherent in investment activities relative to
a complete view of portfolio management. Fuzzy rules are
given where, depending on the antecedents, the portfolio size
may be slightly or significantly decreased or increased. The
decision maker considers acceptable bounds on the proportion
of acceptable risk and return. The Fuzzy Controller model
allows learning to be achieved as 1) the firing strength of each
rule is measured, 2) fuzzy output allows rules to be updated,
and 3) new actions are recommended as the system continues
to loop. An extension is given to the fuzzy controller that
evaluates potential financial loss before adjusting the
portfolio. An application is presented that illustrates the
algorithm and extension developed in the paper.
Abstract: For the electrical metrics that describe photovoltaic
cell performance are inherently multivariate in nature, use of a
univariate, or one variable, statistical process control chart can have
important limitations. Development of a comprehensive process
control strategy is known to be significantly beneficial to reducing
process variability that ultimately drives up the manufacturing cost
photovoltaic cells. The multivariate moving average or MMA chart,
is applied to the electrical metrics of photovoltaic cells to illustrate
the improved sensitivity on process variability this method of control
charting offers. The result show the ability of the MMA chart to
expand to as any variables as needed, suggests an application
with multiple photovoltaic electrical metrics being used in
concert to determine the processes state of control.
Abstract: We describe a novel method for removing noise (in wavelet domain) of unknown variance from microarrays. The method is based on a smoothing of the coefficients of the highest subbands. Specifically, we decompose the noisy microarray into wavelet subbands, apply smoothing within each highest subband, and reconstruct a microarray from the modified wavelet coefficients. This process is applied a single time, and exclusively to the first level of decomposition, i.e., in most of the cases, it is not necessary a multirresoltuion analysis. Denoising results compare favorably to the most of methods in use at the moment.
Abstract: Many corporations are seriously concerned about
security of networks and therefore, their network supervisors are still
reluctant to install WLANs. In this regards, the IEEE802.11i standard
was developed to address the security problems, even though the
mistrust of the wireless LAN technology is still existing. The thought
was that the best security solutions could be found in open standards
based technologies that can be delivered by Virtual Private
Networking (VPN) being used for long time without addressing any
security holes for the past few years. This work, addresses this issue
and presents a simulated wireless LAN of IEEE802.11g protocol, and
analyzes impact of integrating Virtual Private Network technology to
secure the flow of traffic between the client and the server within the
LAN, using OPNET WLAN utility. Two Wireless LAN scenarios
have been introduced and simulated. These are based on normal
extension to a wired network and VPN over extension to a wired
network. The results of the two scenarios are compared and indicate
the impact of improving performance, measured by response time
and load, of Virtual Private Network over wireless LAN.
Abstract: This paper presents a model for the characterization
and selection of beeswaxes for use as base substitute tissue for the
manufacture of objects suitable for external radiotherapy using
megavoltage photon beams. The model of characterization was
divided into three distinct stages: 1) verification of aspects related to
the origin of the beeswax, the bee species, the flora in the vicinity of
the beehives and procedures to detect adulterations; 2) evaluation of
physical and chemical properties; and 3) evaluation of beam
attenuation capacity. The chemical composition of the beeswax
evaluated in this study was similar to other simulators commonly
used in radiotherapy. The behavior of the mass attenuation coefficient
in the radiotherapy energy range was comparable to other simulators.
The proposed model is efficient and enables convenient assessment
of the use of any particular beeswax as a base substitute tissue for
radiotherapy.
Abstract: In this research, an anaerobic co-digestion using decanter cake from palm oil mill industry to improve the biogas production from frozen seafood wastewater is studied using Continuously Stirred Tank Reactor (CSTR) process. The experiments were conducted in laboratory-scale. The suitable Hydraulic Retention Time (HRT) was observed in CSTR experiments with 24 hours of mixing time using the mechanical mixer. The HRT of CSTR process impacts on the efficiency of biogas production. The best performance for biogas production using CSTR process was the anaerobic codigestion for 20 days of HRT with the maximum methane production rate of 1.86 l/d and the average maximum methane production of 64.6%. The result can be concluded that the decanter cake can improve biogas productivity of frozen seafood wastewater.
Abstract: This article provides partial evaluation index and its
standard of sports aerobics, including the following 12 indexes: health
vitality, coordination, flexibility, accuracy, pace, endurance, elasticity,
self-confidence, form, control, uniformity and musicality. The
three-layer BP artificial neural network model including input layer,
hidden layer and output layer is established. The result shows that the
model can well reflect the non-linear relationship between the
performance of 12 indexes and the overall performance. The predicted
value of each sample is very close to the true value, with a relative
error fluctuating around of 5%, and the network training is successful.
It shows that BP network has high prediction accuracy and good
generalization capacity if being applied in sports aerobics performance
evaluation after effective training.
Abstract: A mobile Ad-hoc network consists of wireless nodes
communicating without the need for a centralized administration. A
user can move anytime in an ad hoc scenario and, as a result, such a
network needs to have routing protocols which can adopt
dynamically changing topology. To accomplish this, a number of ad
hoc routing protocols have been proposed and implemented, which
include DSR, OLSR and AODV. This paper presents a study on the
QoS parameters for MANET application traffics in large-scale
scenarios with 50 and 120 nodes. The application traffics analyzed in
this study is File Transfer Protocol (FTP). In large scale networks
(120 nodes) OLSR shows better performance and in smaller scale
networks (50 nodes)AODV shows less packet drop rate and OLSR
shows better throughput.
Abstract: Integrated fiber-wireless (FiWi) access networks are a viable solution that can deliver the high profile quadruple play services. Passive optical networks (PON) networks integrated with wireless access networks provide ubiquitous characteristics for high bandwidth applications. Operation of PON improves by employing a variety of multiplexing techniques. One of it is time division/wavelength division multiplexed (TDM/WDM) architecture that improves the performance of optical-wireless access networks. This paper proposes a novel feedback-based TDM/WDM-PON architecture and introduces a model of integrated PON-FiWi networks. Feedback-based link architecture is an efficient solution to improves the performance of optical-line-terminal (OLT) and interlink optical-network-units (ONUs) communication. Furthermore, the feedback-based WDM/TDM-PON architecture is compared with existing architectures in terms of capacity of network throughput.
Abstract: Dexamethasone (Dex) is a synthetic glucocorticoid
that is used in therapy. However prolonged treatments with high
doses are often required. This causes side effects that interfere with
the activity of several endocrine systems, including the gonadotropic
axis.
The aim of our study is to determine the effect of Dex on testicular
function in prepubertal Wistar rats.
Newborn Wistar rats are submitted to intraperitoneal injection of
Dex (1μg of Dex dissolved in NaCl 0.9% / 5g bw) for 20 days and
then sacrificed at the age of 40days. A control group received NaCl
0.9%. The rat is weighed daily. The plasmatic levels of testosterone,
LH and FSH were measured by radioimmunoassay. A histomorphometric
study was performed on sections of testis.
Treated groups showed a significant decrease in body weight (p
Abstract: Understanding the number of people and the flow of
the persons is useful for efficient promotion of the institution
managements and company-s sales improvements. This paper
introduces an automated method for counting passerby using virtualvertical
measurement lines. The process of recognizing a passerby is
carried out using an image sequence obtained from the USB camera.
Space-time image is representing the human regions which are
treated using the segmentation process. To handle the problem of
mismatching, different color space are used to perform the template
matching which chose automatically the best matching to determine
passerby direction and speed. A relation between passerby speed and
the human-pixel area is used to distinguish one or two passersby. In
the experiment, the camera is fixed at the entrance door of the hall in
a side viewing position. Finally, experimental results verify the
effectiveness of the presented method by correctly detecting and
successfully counting them in order to direction with accuracy of
97%.
Abstract: Hand gesture is one of the typical methods used in
sign language for non-verbal communication. It is most commonly
used by people who have hearing or speech problems to
communicate among themselves or with normal people. Various sign
language systems have been developed by manufacturers around the
globe but they are neither flexible nor cost-effective for the end
users. This paper presents a system prototype that is able to
automatically recognize sign language to help normal people to
communicate more effectively with the hearing or speech impaired
people. The Sign to Voice system prototype, S2V, was developed
using Feed Forward Neural Network for two-sequence signs
detection. Different sets of universal hand gestures were captured
from video camera and utilized to train the neural network for
classification purpose. The experimental results have shown that
neural network has achieved satisfactory result for sign-to-voice
translation.
Abstract: The use of the mechanical simulation (in particular the finite element analysis) requires the management of assumptions in order to analyse a real complex system. In finite element analysis (FEA), two modeling steps require assumptions to be able to carry out the computations and to obtain some results: the building of the physical model and the building of the simulation model. The simplification assumptions made on the analysed system in these two steps can generate two kinds of errors: the physical modeling errors (mathematical model, domain simplifications, materials properties, boundary conditions and loads) and the mesh discretization errors. This paper proposes a mesh adaptive method based on the use of an h-adaptive scheme in combination with an error estimator in order to choose the mesh of the simulation model. This method allows us to choose the mesh of the simulation model in order to control the cost and the quality of the finite element analysis.
Abstract: In the present study, development of salbutamol
sulphate nanoparticles that adhere to gastric mucus was investigated.
Salbutamol sulphate has low bioavailability due to short transit time in
gastric. It also has a positive surface charge that provides hurdles to be
encapsulated by the positively strong mucoadhesive polymer of
chitosan. To overcome the difficulties, the surface charge of active
ingredient was modified using several nonionic and anionic
stomach-specific polymers. The nanoparticles were prepared using
ionotropic gelation technique. The evaluation involved determination
of particle size, zeta potential, entrapment efficiency, in vitro drug
release and in vitro mucoadhesion test. Results exhibited that the use
of anionic alginate polymer was more satisfactory than that of
nonionic polymer. Characteristics of the particles was nano-size, high
encapsulation efficiency, fulfilled the drug release requirements and
adhesive towards stomach for around 11 hours. This result shows that
the salbutamol sulphate nanoparticles can be utilized for improvement
its delivery.
Abstract: Sleep stage scoring is the process of classifying the
stage of the sleep in which the subject is in. Sleep is classified into
two states based on the constellation of physiological parameters.
The two states are the non-rapid eye movement (NREM) and the
rapid eye movement (REM). The NREM sleep is also classified into
four stages (1-4). These states and the state wakefulness are
distinguished from each other based on the brain activity. In this
work, a classification method for automated sleep stage scoring
based on a single EEG recording using wavelet packet decomposition
was implemented. Thirty two ploysomnographic recording from the
MIT-BIH database were used for training and validation of the
proposed method. A single EEG recording was extracted and
smoothed using Savitzky-Golay filter. Wavelet packets
decomposition up to the fourth level based on 20th order Daubechies
filter was used to extract features from the EEG signal. A features
vector of 54 features was formed. It was reduced to a size of 25 using
the gain ratio method and fed into a classifier of regression trees. The
regression trees were trained using 67% of the records available. The
records for training were selected based on cross validation of the
records. The remaining of the records was used for testing the
classifier. The overall correct rate of the proposed method was found
to be around 75%, which is acceptable compared to the techniques in
the literature.
Abstract: The next stage of the home networking environment is
supposed to be ubiquitous, where each piece of material is equipped
with an RFID (Radio Frequency Identification) tag. To fully support
the ubiquitous environment, home networking middleware should be
able to recommend home services based on a user-s interests and
efficiently manage information on service usage profiles for the users.
Therefore, USN (Ubiquitous Sensor Network) technology, which
recognizes and manages a appliance-s state-information (location,
capabilities, and so on) by connecting RFID tags is considered. The
Intelligent Multi-Agent Middleware (IMAM) architecture was
proposed to intelligently manage the mobile RFID-based home
networking and to automatically supply information about home
services that match a user-s interests. Evaluation results for
personalization services for IMAM using Bayesian-Net and Decision
Trees are presented.