Abstract: D-erythro-cyclohexylserine (D
chiral unnatural β-hydroxy amino acid expected for the synthesis of drug for AIDS treatment. To develop a continuous bioconversion
system with whole cell biocatalyst of D-threonine aldolase (D genes for the D-erythro-CHS production, D-threonine aldolase gene
was amplified from Ensifer arboris 100383 by direct PCR amplication using two degenerated oligonucleotide primers designed based on
genomic sequence of Shinorhizobium meliloti
Sequence analysis of the cloned DNA fragment revealed one
open-reading frame of 1059 bp and 386 amino acids. This putative
D-TA gene was cloned into NdeI and EcoRI (pEnsi
His-tag sequence or BamHI (pEnsi-DTA[2])
sequence of the pET21(a) vector. The expression level of the cloned gene was extremely overexpressed by E. coli BL21(DE3) transformed with pEnsi-DTA[1] compared to E. coli BL21(DE3) transformed with
pEnsi-DTA[2]. When the cells expressing the wild
used for D-TA enzyme activity, 12 mM glycine was successfully
detected in HPLC analysis. Moreover, the whole cells harbouring the
recombinant D-TA was able to synthesize D-erythro
of 0.6 mg/ml in a batch reaction.
Abstract: Access control is a critical security service in Wire- less
Sensor Networks (WSNs). To prevent malicious nodes from joining
the sensor network, access control is required. On one hand, WSN
must be able to authorize and grant users the right to access to the
network. On the other hand, WSN must organize data collected by
sensors in such a way that an unauthorized entity (the adversary)
cannot make arbitrary queries. This restricts the network access only
to eligible users and sensor nodes, while queries from outsiders will
not be answered or forwarded by nodes. In this paper we presentee
different access control schemes so as to ?nd out their objectives,
provision, communication complexity, limits, etc. Using the node
density parameter, we also provide a comparison of these proposed
access control algorithms based on the network topology which can
be flat or hierarchical.
Abstract: European Union candidate status provides a
strong motivation for decision-making in the candidate
countries in shaping the regional development policy where
there is an envisioned transfer of power from center to the
periphery. The process of Europeanization anticipates the
candidate countries configure their regional institutional
templates in the context of the requirements of the European
Union policies and introduces new instruments of incentive
framework of enlargement to be employed in regional
development schemes. It is observed that the contribution of
the local actors to the decision making in the design of the
allocation architectures enhances the efficiency of the funds
and increases the positive effects of the projects funded under
the regional development objectives. This study aims at
exploring the performances of the three regional development
grant schemes in Turkey, established and allocated under the
pre-accession process with a special emphasis given to the
roles of the national and local actors in decision-making for
regional development. Efficiency analyses have been
conducted using the DEA methodology which has proved to
be a superior method in comparative efficiency and
benchmarking measurements. The findings of this study as
parallel to similar international studies, provides that the
participation of the local actors to the decision-making in
funding contributes both to the quality and the efficiency of
the projects funded under the EU schemes.
Abstract: Industrial radiography is a famous technique for the identification and evaluation of discontinuities, or defects, such as cracks, porosity and foreign inclusions found in welded joints. Although this technique has been well developed, improving both the inspection process and operating time, it does suffer from several drawbacks. The poor quality of radiographic images is due to the physical nature of radiography as well as small size of the defects and their poor orientation relatively to the size and thickness of the evaluated parts. Digital image processing techniques allow the interpretation of the image to be automated, avoiding the presence of human operators making the inspection system more reliable, reproducible and faster. This paper describes our attempt to develop and implement digital image processing algorithms for the purpose of automatic defect detection in radiographic images. Because of the complex nature of the considered images, and in order that the detected defect region represents the most accurately possible the real defect, the choice of global and local preprocessing and segmentation methods must be appropriated.
Abstract: Information is power. Geographical information is an
emerging science that is advancing the development of knowledge to
further help in the understanding of the relationship of “place" with
other disciplines such as crime. The researchers used crime data for
the years 2004 to 2007 from the Baguio City Police Office to
determine the incidence and actual locations of crime hotspots.
Combined qualitative and quantitative research methodology was
employed through extensive fieldwork and observation, geographic
visualization with Geographic Information Systems (GIS) and Global
Positioning Systems (GPS), and data mining. The paper discusses
emerging geographic visualization and data mining tools and
methodologies that can be used to generate baseline data for
environmental initiatives such as urban renewal and rejuvenation.
The study was able to demonstrate that crime hotspots can be
computed and were seen to be occurring to some select places in the
Central Business District (CBD) of Baguio City. It was observed that
some characteristics of the hotspot places- physical design and milieu
may play an important role in creating opportunities for crime. A list
of these environmental attributes was generated. This derived
information may be used to guide the design or redesign of the urban
environment of the City to be able to reduce crime and at the same
time improve it physically.
Abstract: Water contains oxygen which may make a human
breathe under water like a fish. Centrifugal separator can separate
dissolved gases from water. Carrier solution can increase the
separation of dissolved oxygen from water. But, to develop an
breathing device for a human under water, the enhancement of
separation of dissolved gases including oxygen and portable devices
which have dc battery based device and proper size are needed.
In this study, we set up experimental device for analyzing
separation characteristics of dissolved gases including oxygen from
water using a battery based portable vacuum pump. We characterized
vacuum state, flow rate of separation of dissolved gases and oxygen
concentration which were influenced by the manufactured vacuum
pump.
Abstract: The paper presents an overview of environmental
issues that may be expected with nuclear desalination. The analysis
of coupling nuclear power with desalination plants indicates that
adverse marine impacts can be mitigated with alternative intake
designs or cooling systems. The atmospheric impact of desalination
may be greatly reduced through the coupling with nuclear power,
while maximizing the socio-economic benefit for both processes. The
potential for tritium contamination of the desalinated water was
reviewed. Experience with the systems and practices related to the
radiological quality of the product water, shows no examples of
cross-contamination. Furthermore, the indicators for the public
acceptance of nuclear desalination, as one of the most important
sustainability aspects of any such large project, show a positive trend.
From the data collected, a conclusion is made that nuclear
desalination should be supported by decision-makers.
Abstract: Atrial Fibrillation is the most common sustained
arrhythmia encountered by clinicians. Because of the invisible
waveform of atrial fibrillation in atrial activation for human, it is
necessary to develop an automatic diagnosis system. 12-Lead ECG
now is available in hospital and is appropriate for using Independent
Component Analysis to estimate the AA period. In this research, we
also adopt a second-order blind identification approach to transform
the sources extracted by ICA to more precise signal and then we use
frequency domain algorithm to do the classification. In experiment,
we gather a significant result of clinical data.
Abstract: In this paper is investigated a possible
optimization of some linear algebra problems which can be
solved by parallel processing using the special arrays called
systolic arrays. In this paper are used some special types of
transformations for the designing of these arrays. We show
the characteristics of these arrays. The main focus is on
discussing the advantages of these arrays in parallel
computation of matrix product, with special approach to the
designing of systolic array for matrix multiplication.
Multiplication of large matrices requires a lot of
computational time and its complexity is O(n3 ). There are
developed many algorithms (both sequential and parallel) with
the purpose of minimizing the time of calculations. Systolic
arrays are good suited for this purpose. In this paper we show
that using an appropriate transformation implicates in finding
more optimal arrays for doing the calculations of this type.
Abstract: The purposes of this research were 1) to survey the
number of drugstores that unlawful dispense of asthma prescription
drugs, in form of drug combinations in the Phaya Thai district of
Bangkok, 2) to find the steroids contained in that drug combinations,
3) to find a means for informing general public about the dangers of
drugs and for a campaign to stop dispensing them.
Researcher collected drug combinations from 69 drugstores in
Phaya Thai district from Feb 15, 2012 to Mar 15, 2012. The survey
found 30.43%, 21, drug stores, sold asthma drug combinations to
customers without a prescription. These collected samples were
tested for steroid contamination by using Immunochromatography
kits. Eleven samples, 52.38%, were found contaminated with
steroids. In short, there should be control and inspection of
drugstores in the distribution of steroid medications. To improve the
knowledge of self health maintenance and drug usage among public,
Thai Government and Department of Public Health should educate
people about the side effects of using drug combinations and steroids.
Abstract: Adhesively bonded joints are preferred over the
conventional methods of joining such as riveting, welding, bolting
and soldering. Some of the main advantages of adhesive joints
compared to conventional joints are the ability to join dissimilar
materials and damage-sensitive materials, better stress distribution,
weight reduction, fabrication of complicated shapes, excellent
thermal and insulation properties, vibration response and enhanced
damping control, smoother aerodynamic surfaces and an
improvement in corrosion and fatigue resistance. This paper presents
the behavior of adhesively bonded joints subjected to combined
thermal loadings, using the numerical methods. The joint
configuration considers aluminum as central adherend with six
different outer adherends including aluminum, steel, titanium, boronepoxy,
unidirectional graphite-epoxy and cross-ply graphite-epoxy
and epoxy-based adhesives. Free expansion of the joint in x
direction was permitted and stresses in adhesive layer and interfaces
calculated for different adherends.
Abstract: A new multi inner stage (MIS) cyclone was designed to
remove the acidic gas and fine particles produced from electronic
industry. To characterize gas flow in MIS cyclone, pressure and
velocity distribution were calculated by means of CFD program. Also,
the flow locus of fine particles and particle removal efficiency were
analyzed by Lagrangian method. When outlet pressure condition was
–100mmAq, the efficiency was the best in this study.
Abstract: Proper management of residues originated from
industrial activities is considered as one of the serious challenges
faced by industrial societies due to their potential hazards to the
environment. Common disposal methods for industrial solid wastes
(ISWs) encompass various combinations of solely management
options, i.e. recycling, incineration, composting, and sanitary
landfilling. Indeed, the procedure used to evaluate and nominate the
best practical methods should be based on environmental, technical,
economical, and social assessments. In this paper an environmentaltechnical
assessment model is developed using analytical network
process (ANP) to facilitate the decision making practice for ISWs
generated at Gilan province, Iran. Using the results of performed
surveys on industrial units located at Gilan, the various groups of
solid wastes in the research area were characterized, and four
different ISW management scenarios were studied. The evaluation
process was conducted using the above-mentioned model in the
Super Decisions software (version 2.0.8) environment. The results
indicates that the best ISW management scenario for Gilan province
is consist of recycling the metal industries residues, composting the
putrescible portion of ISWs, combustion of paper, wood, fabric and
polymeric wastes as well as energy extraction in the incineration
plant, and finally landfilling the rest of the waste stream in addition
with rejected materials from recycling and compost production plants
and ashes from the incineration unit.
Abstract: The phenomenon of global warming or climate
change has led to many environmental issues including higher
atmospheric temperatures, intense precipitation, increased
greenhouse gaseous emissions and increased indoor discomfort.
Studies have shown that bringing nature to the roof such as
constructing green roof and implementing high-reflective roof may
give positive impact in mitigating the effects of global warming and
in increasing thermal comfort sensation inside buildings. However,
no study has been conducted to compare both types of passive roof
treatments in Malaysia in order to increase thermal comfort in
buildings. Therefore, this study is conducted to investigate the effect
of green roof and white painted roof as passive roof treatment in
improving indoor comfort of Malaysian homes. This study uses an
experimental approach in which the measurements of temperatures
are conducted on the case study building. The measurements of
outdoor and indoor environments were conducted on the flat roof
with two different types of roof treatment that are green roof and
white roof. The measurement of existing black bare roof was also
conducted to act as a control for this study.
Abstract: Prediction of bacterial virulent protein sequences can
give assistance to identification and characterization of novel
virulence-associated factors and discover drug/vaccine targets against
proteins indispensable to pathogenicity. Gene Ontology (GO)
annotation which describes functions of genes and gene products as a
controlled vocabulary of terms has been shown effectively for a
variety of tasks such as gene expression study, GO annotation
prediction, protein subcellular localization, etc. In this study, we
propose a sequence-based method Virulent-GO by mining informative
GO terms as features for predicting bacterial virulent proteins.
Each protein in the datasets used by the existing method
VirulentPred is annotated by using BLAST to obtain its homologies
with known accession numbers for retrieving GO terms. After
investigating various popular classifiers using the same five-fold
cross-validation scheme, Virulent-GO using the single kind of GO
term features with an accuracy of 82.5% is slightly better than
VirulentPred with 81.8% using five kinds of sequence-based features.
For the evaluation of independent test, Virulent-GO also yields better
results (82.0%) than VirulentPred (80.7%). When evaluating single
kind of feature with SVM, the GO term feature performs much well,
compared with each of the five kinds of features.
Abstract: Biofuels, like biobutanol, have been recognized for
being renewable and sustainable fuels which can be produced from
lignocellulosic biomass. To convert lignocellulosic biomass to
biofuel, pretreatment process is an important step to remove
hemicelluloses and lignin to improve enzymatic hydrolysis. Dilute
acid pretreatment has been successful developed for pretreatment of
corncobs and the optimum conditions of dilute sulfuric and
phosphoric acid pretreatment were obtained at 120 °C for 5 min with
15:1 liquid to solid ratio and 140 °C for 10 min with 10:1 liquid to
solid ratio, respectively. The result shows that both of acid
pretreatments gave the content of total sugar approximately 34–35
g/l. In case of inhibitor content (furfural), phosphoric acid
pretreatment gives higher than sulfuric acid pretreatment.
Characterizations of corncobs after pretreatment indicate that both of
acid pretreatments can improve enzymatic accessibility and the better
results present in corncobs pretreated with sulfuric acid in term of
surface area, crystallinity, and composition analysis.
Abstract: The last Assessment Report of the Intergovernmental
Panel on Climate Change, stating that the greatest risk in climate
change affects sustainability is now widely known and accepted.
However, it has not provoked substantial reaction and attention in
Hungary, while international and national efforts have also not
achieved expected results so far. Still, there are numerous examples
on different levels (national, regional, local, household) making
considerable progress in limiting their own emissions and making
steps toward mitigation of and adaptation to climate change. The
local level is exceptionally important in sustainability adaptation, as
local communities are often able to adapt more flexibly to changes in
the natural environment.The aim of this paper is to attempt a review
of the national climate policy and the local climate change strategies
in Hungary considering sustainable development.
Abstract: Recent trends in building constructions in Libya are
more toward tall (high-rise) building projects. As a consequence, a
better estimation of the lateral loading in the design process is
becoming the focal of a safe and cost effective building industry. Byin-
large, Libya is not considered a potential earthquake prone zone,
making wind is the dominant design lateral loads. Current design
practice in the country estimates wind speeds on a mere random
bases by considering certain factor of safety to the chosen wind
speed. Therefore, a need for a more accurate estimation of wind
speeds in Libya was the motivation behind this study. Records of
wind speed data were collected from 22 metrological stations in
Libya, and were statistically analysed. The analysis of more than four
decades of wind speed records suggests that the country can be
divided into four zones of distinct wind speeds. A computer “survey"
program was manipulated to draw design wind speeds contour map
for the state of Libya.
The paper presents the statistical analysis of Libya-s recorded
wind speed data and proposes design wind speed values for a 50-year
return period that covers the entire country.
Abstract: Fermented cassava flours (lafun) sold in Ogun and Oyo
States of Nigeria were collected from 10 markets for a period of two
months and analysed to determine their safety status. The presence of
trace metals was due to high vehicular movement around the drying
sites and markets. Cyanide and moisture contents of samples were
also determined to assess the adequacy of fermentation and drying.
The result showed that sample OWO was found to have the highest
amount of 16.02±0.12mg/kg cyanide while the lowest was found in
sample OJO with 10.51±0.10mg/kg. The results also indicated that
sample TVE had the highest moisture content of 18.50±0.20% while
sample OWO had the lowest amount of 12.46±0.47%. Copper and
lead levels were found to be highest in TVE with values 28.10mg/kg
and 1.1mg/kg respectively, while sample BTS had the lowest values
of 20.6mg/kg and 0.05mg/kg respectively. High value of cyanide
indicated inadequate fermentation.
Abstract: In this paper, we propose a face recognition algorithm
using AAM and Gabor features. Gabor feature vectors which are well
known to be robust with respect to small variations of shape, scaling,
rotation, distortion, illumination and poses in images are popularly
employed for feature vectors for many object detection and
recognition algorithms. EBGM, which is prominent among face
recognition algorithms employing Gabor feature vectors, requires
localization of facial feature points where Gabor feature vectors are
extracted. However, localization method employed in EBGM is based
on Gabor jet similarity and is sensitive to initial values. Wrong
localization of facial feature points affects face recognition rate. AAM
is known to be successfully applied to localization of facial feature
points. In this paper, we devise a facial feature point localization
method which first roughly estimate facial feature points using AAM
and refine facial feature points using Gabor jet similarity-based facial
feature localization method with initial points set by the rough facial
feature points obtained from AAM, and propose a face recognition
algorithm using the devised localization method for facial feature
localization and Gabor feature vectors. It is observed through
experiments that such a cascaded localization method based on both
AAM and Gabor jet similarity is more robust than the localization
method based on only Gabor jet similarity. Also, it is shown that the
proposed face recognition algorithm using this devised localization
method and Gabor feature vectors performs better than the
conventional face recognition algorithm using Gabor jet
similarity-based localization method and Gabor feature vectors like
EBGM.