Screening and Evaluation of in vivo and in vitro Generated Insulin Plant (Vernonia divergens) for Antimicrobial and Anticancer Activities

Vernonia divergens Benth., commonly known as “Insulin Plant” (Fam: Asteraceae) is a potent sugar killer. Locally the leaves of the plant, boiled in water are successfully administered to a large number of diabetic patients. The present study evaluates the putative anti-diabetic ingredients, isolated from the in vivo and in vitro grown plantlets of V. divergens for their antimicrobial and anticancer activities. Sterilized explants of nodal segments were cultured on MS (Musashige and Skoog, 1962) medium in presence of different combinations of hormones. Multiple shoots along with bunch of roots were regenerated at 1mg l-1 BAP and 0.5 mg l-1 NAA. Micro-plantlets were separated and sub-cultured on the double strength (2X) of the above combination of hormones leading to increased length of roots and shoots. These plantlets were successfully transferred to soil and survived well in nature. The ethanol extract of plantlets from both in vivo & in vitro sources were prepared in soxhlet extractor and then concentrated to dryness under reduced pressure in rotary evaporator. Thus obtainedconcentrated extracts showed significant inhibitory activity against gram negative bacteria like Escherichia coli and Pseudomonas aeruginosa but no inhibition was found against gram positive bacteria. Further, these ethanol extracts were screened for in vitro percentage cytotoxicity at different time periods (24 h, 48 h and 72 h) of different dilutions. The in vivo plant extract inhibited the growth of EAC mouse cell lines in the range of 65, 66, 78, and 88% at 100, 50, 25 & 12.5μg mL-1 but at 72 h of treatment. In case of the extract of in vitro origin, the inhibition was found against EAC cell lines even at 48h. During spectrophotometric scanning, the extracts exhibited different maxima (ʎ) - four peaks in in vitro extracts as against single in in vivo preparation suggesting the possible change in the nature of ingredients during micropropagation through tissue culture techniques.

Modeling Spatial Distributions of Point and Nonpoint Source Pollution Loadings in the Great Lakes Watersheds

A physically based, spatially-distributed water quality model is being developed to simulate spatial and temporal distributions of material transport in the Great Lakes Watersheds of the U.S. Multiple databases of meteorology, land use, topography, hydrography, soils, agricultural statistics, and water quality were used to estimate nonpoint source loading potential in the study watersheds. Animal manure production was computed from tabulations of animals by zip code area for the census years of 1987, 1992, 1997, and 2002. Relative chemical loadings for agricultural land use were calculated from fertilizer and pesticide estimates by crop for the same periods. Comparison of these estimates to the monitored total phosphorous load indicates that both point and nonpoint sources are major contributors to the total nutrient loads in the study watersheds, with nonpoint sources being the largest contributor, particularly in the rural watersheds. These estimates are used as the input to the distributed water quality model for simulating pollutant transport through surface and subsurface processes to Great Lakes waters. Visualization and GIS interfaces are developed to visualize the spatial and temporal distribution of the pollutant transport in support of water management programs.

Optimisation of Polycyclic AromaticHydrocarbon Removal from Contaminated Soilusing Modified Fenton Treatment

The performance of modified Fenton (MF) treatment to promote PAH oxidation in artificially contaminated soil was investigated in packed soil column with a hydrogen peroxide (H2O2) delivery system simulating in situ injection. Soil samples were spiked with phenanthrene (low molecular weight PAH) and fluoranthene (high molecular weight PAH) to an initial concentration of 500 mg/kg dried soil each. The effectiveness of process parameters H2O2/soil, iron/soil, chelating agent/soil weight ratios and reaction time were studied using a 24 three level factorial design experiments. Statistically significant quadratic models were developed using Response Surface Methodology (RSM) for degrading PAHs from the soil samples. Optimum operating condition was achieved at mild range of H2O2/soil, iron/soil and chelating agent/soil weight ratios, indicating cost efficient method for treating highly contaminated lands.

Frequency Response Analysis of Reinforced- Soil Retaining Walls with Polymeric Strips

Few studies have been conducted on polymeric strip and the behavior of soil retaining walls. This paper will present the effect of frequency on the dynamic behavior of reinforced soil retaining walls with polymeric strips. The frequency content describes how the amplitude of a ground motion is distributed among different frequencies. Since the frequency content of an earthquake motion will strongly influence the effects of that motion, the characterization of the motion cannot be completed without the consideration of its frequency content. The maximum axial force of reinforcements and horizontal displacement of the reinforced walls are focused in this research. To clarify the dynamic behavior of reinforced soil retaining walls with polymeric strips, a numerical modeling using Finite Difference Method is benefited. As the results indicate, the frequency of input base acceleration has an important effect on the behavior of these structures. Because of resonant in the system, where the frequency of the input dynamic load is equal to the natural frequency of the system, the maximum horizontal displacement and the maximum axial forces in polymeric strips is occurred. Moreover, they were to increase the structure flexibility because of the main advantages of polymeric strips; i.e. being simple method of construction, having a homogeneous behavior with soils, and possessing long durability, which are of great importance in dynamic analysis.

A Simulation Model for the H-gate PDSOI MOSFET

The floating body effect is a serious problem for the PDSOI MOSFET, and the H-gate layout is frequently used as the body contact to eliminate this effect. Unfortunately, most of the standard commercial SOI MOSFET model is for the device with finger gate, the necessity of the new models for the H-gate device arises. A simulation model for the H-gate PDSOI MOSFET is proposed based on the 0.35μm PDSOI process developed by the Institute of Microelectronics of the Chinese Academy of Sciences (IMECAS), and then the model is well verified by the ring-oscillator.

Threshold Stress of the Soil Subgrade Evaluation for Highway Formations

The objective of this study is to evaluate the threshold stress of the clay with sand subgrade soil. Threshold stress can be defined as the stress level above which cyclic loading leads to excessive deformation and eventual failure. The thickness determination of highways formations using the threshold stress approach is a more realistic assessment of the soil behaviour because it is subjected to repeated loadings from moving vehicles. Threshold stress can be evaluated by plastic strain criterion, which is based on the accumulated plastic strain behaviour during cyclic loadings [1]. Several conditions of the all-round pressure the subgrade soil namely, zero confinement, low all-round pressure and high all-round pressure are investigated. The threshold stresses of various soil conditions are determined. Threshold stress of the soil are 60%, 31% and 38.6% for unconfined partially saturated sample, low effective stress saturated sample, high effective stress saturated sample respectively.

Experimental Study on Damping Ratios of in-situ Buildings

Accurate evaluation of damping ratios involving soilstructure interaction (SSI) effects is the prerequisite for seismic design of in-situ buildings. This study proposes a combined approach to identify damping ratios of SSI systems based on ambient excitation technique. The proposed approach is illustrated with main test process, sampling principle and algorithm steps through an engineering example, as along with its feasibility and validity. The proposed approach is employed for damping ratio identification of 82 buildings in Xi-an, China. Based on the experimental data, the variation range and tendency of damping ratios of these SSI systems, along with the preliminary influence factor, are shown and discussed. In addition, a fitting curve indicates the relation between the damping ratio and fundamental natural period of SSI system.

Determination of Alkaline Protease Production In Serratia Marcescens Sp7 Using Agro Wastes As Substrate Medium, Optimization Of Production Parameters And Purification Of The Enzyme

The enzyme alkaline protease production was determined under solid state fermentation using the soil bacteria Serratia marcescens sp7. The maximum production was obtained from wheat bran medium than ground nut shell and chemically defined medium. The physiological fermentation factors such as pH of the medium (pH 8), Temperature (40oC) and incubation time (48 hrs) played a vital role in alkaline protease production in all the above. 100Mm NaCl has given better resolution during elution of the enzymes. The enzyme production was found to be associated with growth of the bacterial culture.

Importance of Pastoral Human Factor Overloading in Land Desertification: Case Studies in Northeastern Libya

Grazing and pastoral overloading through human factors result in significant land desertification. Failure to take into account the phenomenon of desertification as a serious problem can lead to an environmental disaster because of the damages caused by land encroachment. Therefore, soil on residential and urban areas is affected because of the deterioration of vegetation. Overgrazing or grazing in open and irregular lands is practiced in these areas almost throughout the year, especially during the growth cycle of edible plants, thereby leading to their disappearance. In addition, the large number of livestock in these areas exceeds the capacity of these pastures because of pastoral land overloading, which results in deterioration and desertification in the region. In addition, rare plants, the extinction of some edible plants in the region, and the emergence of plants unsuitable for grazing, must be taken into consideration, as along with the emergence of dust and sand storms during the dry seasons (summer to autumn) due to the degradation of vegetation. These results show that strategic plans and regulations that protect the environment from desertification must be developed. Therefore, increased pastoral load is a key human factor in the deterioration of vegetation cover, leading to land desertification in this region.

Effect of Buoyancy Ratio on Non-Darcy Mixed Convection in a Vertical Channel: A Thermal Non-equilibrium Approach

This article presents a numerical study of the doublediffusive mixed convection in a vertical channel filled with porous medium by using non-equilibrium model. The flow is assumed fully developed, uni-directional and steady state. The controlling parameters are thermal Rayleigh number (RaT ), Darcy number (Da), Forchheimer number (F), buoyancy ratio (N), inter phase heat transfer coefficient (H), and porosity scaled thermal conductivity ratio (γ). The Brinkman-extended non-Darcy model is considered. The governing equations are solved by spectral collocation method. The main emphasize is given on flow profiles as well as heat and solute transfer rates, when two diffusive components in terms of buoyancy ratio are in favor (against) of each other and solid matrix and fluid are thermally non-equilibrium. The results show that, for aiding flow (RaT = 1000), the heat transfer rate of fluid (Nuf ) increases upto a certain value of H, beyond that decreases smoothly and converges to a constant, whereas in case of opposing flow (RaT = -1000), the result is same for N = 0 and 1. The variation of Nuf in (N, Nuf )-plane shows sinusoidal pattern for RaT = -1000. For both cases (aiding and opposing) the flow destabilize on increasing N by inviting point of inflection or flow separation on the velocity profile. Overall, the buoyancy force have significant impact on the non-Darcy mixed convection under LTNE conditions.

Three-Level Converters based Generalized Unified Power Quality Conditioner

A generalized unified power quality conditioner (GUPQC) by using three single-phase three-level voltage source converters (VSCs) connected back-to-back through a common dc link is proposed in this paper as a new custom power device for a three-feeder distribution system. One of the converters is connected in shunt with one feeder for mitigation of current harmonics and reactive power compensation, while the other two VSCs are connected in series with the other two feeders to maintain the load voltage sinusoidal and at constant level. A new control scheme based on synchronous reference frame is proposed for series converters. The simulation analysis on compensation performance of GUPQC based on PSCAD/EMTDC is reported.

Microwave Shielding of Magnetized Hydrogen Plasma in Carbon Nanotubes

We derive simple sets of equations to describe the microwave response of a thin film of magnetized hydrogen plasma in the presence of carbon nanotubes, which were grown by ironcatalyzed high-pressure disproportionation (HiPco). By considering the interference effects due to multiple reflections between thin plasma film interfaces, we present the effects of the continuously changing external magnetic field and plasma parameters on the reflected power, absorbed power, and transmitted power in the system. The simulation results show that the interference effects play an important role in the reflectance, transmittance and absorptance of microwave radiation at the magnetized plasma slab. As a consequence, the interference effects lead to a sinusoidal variation of the reflected intensity and can greatly reduce the amount of reflection power, but the absorption power increases.

Determination of Some Organochlorine Pesticide Residues in Vegetable and Soil Samples from Alau Dam and Gongulong Agricultural Sites, Borno State, North Eastern Nigeria

Five vegetables (spinach, lettuce, cabbage, tomato, and onion) were freshly harvested from the Alau Dam and Gongulong agricultural areas for the determination of some organochlorine pesticide residues (o, p-DDE, p,p’-DDD, o,p’-DDD, p,p’-DDT, α-BHC, γ-BHC, metoxichlor, lindane, endosulfan dieldrin, and aldrin.) Soil samples were also collected at different depths for the determination of the above pesticides. Samples collection and preparation were conducted using standard procedures. The concentrations of all the pesticides in the soil and vegetable samples were determined using GC/MS SHIMADZU (GC-17A) equipped with electron capture detector (ECD). The highest concentration was that of p,p’-DDD (132.4±13.45µg/g) which was observed in the leaf of cabbage, while the lowest concentration was that of p,p’-DDT (2.34µg/g) was observed in the root of spinach. Similar trends were observed at the Gongulong agricultural area, with p,p’-DDD having the highest concentration of 153.23µg/g in the leaf of cabbage, while the lowest concentration was that of p,p’-DDT (12.45µg/g) which was observed in the root of spinach. α-BHC, γ-BHC, Methoxychlor, and lindane were detected in all the vegetable samples studied. The concentrations of all the pesticides in the soil samples were observed to be higher at a depth of 21-30cm, while the lowest concentrations were observed at a depth of 0-10cm. The concentrations of all the pesticides in the vegetables and soil samples from the two agricultural sites were observed to be at alarming levels, much higher than the maximum residue limits (MRLs) and acceptable daily intake values (ADIs) .The levels of the pesticides observed in the vegetables and soil samples investigated, are of such a magnitude that calls for special attention and laws to regulate the use and circulation of such chemicals. Routine monitoring of pesticide residues in these study areas is necessary for the prevention, control and reduction of environmental pollution, so as to minimize health risks.

Conservation Techniques for Soil Erosion Control in Tobacco-Based Farming System at Steep Land Areas of Progo Hulu Subwatershed, Central Java, Indonesia

This research was aimed at determining the impact of conservation techniques including bench terrace, stone terrace, mulching, grass strip and intercropping on soil erosion at tobacco-based farming system at Progo Hulu subwatershed, Central Java, Indonesia. Research was conducted from September 2007 to September 2009, located at Progo Hulu subwatershed, Central Java, Indonesia. Research site divided into 27 land units, and experimental fields were grouped based on the soil type and slope, ie: 30%, 45% and 70%, with the following treatments: 1) ST0= stone terrace (control); 2) ST1= stone terrace + Setaria spacelata grass strip on a 5 cm height dike at terrace lips + tobacco stem mulch with dose of 50% (7 ton/ ha); 3) ST2= stone terrace + Setaria spacelata grass strip on a 5 cm height dike at terrace lips + tobacco stem mulch with dose of 100% (14 ton/ ha); 4) ST3= stone terrace + tobacco and red bean intercropping + tobacco stem mulch with dose of 50% (7 ton/ ha). 5) BT0= bench terrace (control); 6) BT1= bench terrace + Setaria spacelata grass strip at terrace lips + tobacco stem mulch with dose of 50% (7 ton/ ha); 7) BT2= bench terrace + Setaria spacelata grass strip at terrace lips + tobacco stem mulch with dose of 100% (14 ton/ ha); 8) BT3= bench terrace + tobacco and red bean intercropping + tobacco stem mulch with dose of 50% (7 ton/ ha). The results showed that the actual erosion rates of research site were higher than that of tolerance erosion with mean value 89.08 ton/ha/year and 33.40 ton/ha/year, respectively. These resulted in 69% of total research site (5,119.15 ha) highly degraded. Conservation technique of ST2 was the most effective in suppressing soil erosion, by 42.87%, following with BT2 as much 30.63%. Others suppressed erosion only less than 21%.

Comparison of Finite Difference Schemes for Water Flow in Unsaturated Soils

Flow movement in unsaturated soil can be expressed by a partial differential equation, named Richards equation. The objective of this study is the finding of an appropriate implicit numerical solution for head based Richards equation. Some of the well known finite difference schemes (fully implicit, Crank Nicolson and Runge-Kutta) have been utilized in this study. In addition, the effects of different approximations of moisture capacity function, convergence criteria and time stepping methods were evaluated. Two different infiltration problems were solved to investigate the performance of different schemes. These problems include of vertical water flow in a wet and very dry soils. The numerical solutions of two problems were compared using four evaluation criteria and the results of comparisons showed that fully implicit scheme is better than the other schemes. In addition, utilizing of standard chord slope method for approximation of moisture capacity function, automatic time stepping method and difference between two successive iterations as convergence criterion in the fully implicit scheme can lead to better and more reliable results for simulation of fluid movement in different unsaturated soils.

Determination of Moisture Content and Liquid Limit of Foundations Soils, using Microwave Radiation, in the Different Locations of Sulaimani Governorate, Kurdistan Region-Iraq

Soils are normally dried in either a convection oven or stove. Laboratory moisture content testing indicated that the typical drying durations for a convection oven were, 24 hours. The purpose of this study was to determine the accuracy and soil drying duration of both, moisture content and liquid limit using microwave radiation. The soils were tested with both, convection and microwave ovens. The convection oven was considered to produce the true values for both, natural moisture content and liquid limit of soils; it was, therefore, used as a basis for comparison for the results of the microwave ovens. The samples used in this study were obtained from different projects of Consulting Engineering Bureau of College of Engineering of Sulaimani University. These samples were collected from different locations and at the different depths and consist mostly of brown and light brown clay and silty clay. A total of 102 samples were prepared. 26 of them were tested for natural moisture determination, while the other 76 were used for liquid limits determination

Facial Expressions Recognition from Complex Background using Face Context and Adaptively Weighted sub-Pattern PCA

A new approach for facial expressions recognition based on face context and adaptively weighted sub-pattern PCA (Aw-SpPCA) has been presented in this paper. The facial region and others part of the body have been segmented from the complex environment based on skin color model. An algorithm has been proposed to accurate detection of face region from the segmented image based on constant ratio of height and width of face (δ= 1.618). The paper also discusses on new concept to detect the eye and mouth position. The desired part of the face has been cropped to analysis the expression of a person. Unlike PCA based on a whole image pattern, Aw-SpPCA operates directly on its sub patterns partitioned from an original whole pattern and separately extracts features from them. Aw-SpPCA can adaptively compute the contributions of each part and a classification task in order to enhance the robustness to both expression and illumination variations. Experiments on single standard face with five types of facial expression database shows that the proposed method is competitive.

The Effect of Geometry Dimensions on the Earthquake Response of the Finite Element Method

In this paper, the effect of width and height of the model on the earthquake response in the finite element method is discussed. For this purpose an earth dam as a soil structure under earthquake has been considered. Various dam-foundation models are analyzed by Plaxis, a finite element package for solving geotechnical problems. The results indicate considerable differences in the seismic responses.

Genetic Combined with a Simplex Algorithm as an Efficient Method for the Detection of a Depressed Ellipsoidal Flaw using the Boundary Element Method

The present work encounters the solution of the defect identification problem with the use of an evolutionary algorithm combined with a simplex method. In more details, a Matlab implementation of Genetic Algorithms is combined with a Simplex method in order to lead to the successful identification of the defect. The influence of the location and the orientation of the depressed ellipsoidal flaw was investigated as well as the use of different amount of static data in the cost function. The results were evaluated according to the ability of the simplex method to locate the global optimum in each test case. In this way, a clear impression regarding the performance of the novel combination of the optimization algorithms, and the influence of the geometrical parameters of the flaw in defect identification problems was obtained.

Modeling of Fluid Flow in 2D Triangular, Sinusoidal, and Square Corrugated Channels

The main focus of the work was concerned with hydrodynamic and thermal analysis of the plate heat exchanger channel with corrugation patterns suggested to be triangular, sinusoidal, and square corrugation. This study was to numerically model and validate the triangular corrugated channel with dimensions/parameters taken from open literature, and then model/analyze both sinusoidal, and square corrugated channel referred to the triangular model. Initially, 2D modeling with local extensive analysis for triangular corrugated channel was carried out. By that, all local pressure drop, wall shear stress, friction factor, static temperature, heat flux, Nusselt number, and surface heat coefficient, were analyzed to interpret the hydrodynamic and thermal phenomena occurred in the flow. Furthermore, in order to facilitate confidence in this model, a comparison between the values predicted, and experimental results taken from literature for almost the same case, was done. Moreover, a holistic numerical study for sinusoidal and square channels together with global comparisons with triangular corrugation under the same condition, were handled. Later, a comparison between electric, and fluid cooling through varying the boundary condition was achieved. The constant wall temperature and constant wall heat flux boundary conditions were employed, and the different resulted Nusselt numbers as a consequence were justified. The results obtained can be used to come up with an optimal design, a 'compromise' between heat transfer and pressure drop.