Abstract: In this paper we evaluated the efficacy of
photodynamic treatment of infected wounds on pig animal model by
diffuse reflectance spectrometry. The study was conducted on fifteen
wounds contaminated with Staphylococcus aureus bacteria that were
incubated for 30 min with methylene blue solution (c = 3.3 x 10-3 M)
and exposed to laser radiations (λ = 670 nm, P = 15 mW) for 15 min.
The efficiency of photodynamic inactivation of bacteria was
evaluated by microbiological exams and diffuse reflectance
spectrometry. The results of the microbiological exams showed that
the bacterial concentration has decreased from 6.93±0.138
logCFU/ml to 3.12±0.108 logCFU/ml. The spectral examination
showed that the diffuse reflectance of wounds contaminated with
Staphylococcus aureus has decreased from 5.06±0.036 % to
3.36±0.025 %. In conclusion, photodynamic therapy is an effective
method for the treatment of infected wounds and there is a correlation
between the CFU count and diffuse reflectance.
Abstract: The systematic manipulations of shapes and sizes of
inorganic compounds greatly benefit the various application fields
including optics, magnetic, electronics, catalysis and medicine.
However shape control has been much more difficult to achieve.
Hence exploration of novel method for the preparation of differently
shaped nanoparticles is challenging research area. II-VI group of
semiconductor cadmium sulphide (CdS) nanostructure with different
morphologies (such as, acicular like, mesoporous, spherical shapes)
and of crystallite sizes vary from 11 to 16 nm were successfully
synthesized by chemical aqueous precipitation of Cd2+ ions with
homogeneously released S2- ions from decomposition of cadmium
sulphate (CdSO4) and thioacetamide (CH3CSNH2) by annealing at
different radiations (microwave, ultrasonic and sunlight) with matter
and systematic research has been done for various factors affecting
the controlled growth rate of CdS nanoparticles. The obtained
nanomaterials have been characterized by X-ray Diffraction (XRD),
Fourier Transform Infrared Spectroscopy (FTIR),
Thermogravometric (DSC-TGA) analysis and Scanning Electron
Microscopy (SEM). The result indicates that on increasing the
reaction time particle size increases but on increasing the molar ratios
grain size decreases.