Abstract: Doxorubicin, also known as Adriamycin, is an
anthracycline class of drug used in cancer chemotherapy. It is used in
the treatment of non-Hodgkin’s lymphoma, multiple myeloma, acute
leukemia, breast cancer, lung cancer, endometrium cancer and ovary
cancers. It functions via intercalating DNA and ultimately killing
cancer cells. The major side effects of doxorubicin are hair loss,
myelosuppression, nausea & vomiting, oesophagitis, diarrhea, heart
damage and liver dysfunction. The minor modifications in the
structure of compound exhibit large variation in the biological
activity, has prompted us to carry out the synthesis of sulfonamide
derivatives. Sulfonamide is an important feature with broad spectrum
of biological activity such as antiviral, antifungal, diuretics, antiinflammatory,
antibacterial and anticancer activities. Structure of the
synthesized compound N-(1-methyl-2-oxo-2-N-methyl anilinoethyl)
benzene sulfonamide confirmed by proton nuclear magnetic
resonance (1H NMR),13C NMR, Mass and FTIR spectroscopic tools
to assure the position of all protons and hence stereochemistry of the
molecule. Further we have reported the binding potential of
synthesized sulfonamide analogues in comparison to doxorubicin
drug using Auto Dock 4.2 software. Computational binding energy
(B.E.) and inhibitory constant (Ki) has been evaluated for the
synthesized compound in comparison of doxorubicin against Poly
(dA-dT).Poly (dA-dT) and Poly (dG-dC).Poly (dG-dC) sequences.
The in vitro cytotoxic study against human breast cancer cell lines
confirms the better anticancer activity of the synthesized compound
over currently in use anticancer drug doxorubicin. The IC50 value of
the synthesized compound is 7.12 μM whereas for doxorubicin is 7.2
μM.
Abstract: Since, it is essential to provide homeless people by the
earthquake with safe, habitable accommodation repairing medium
and slight levels of damage at the connection parts should be
undertaken. In order to prove that a repaired connection was
sufficiently strong, a precast beam to column post tensioned
connection was tested in three phases. In phase one, the middle level
damage was observed at 6% drift at these connections. As a result of
the extra loads applied, little damage was observed. In the last phase,
the four connections tested in the first phase were repaired using
epoxy resin and then retested. The results from the tests on the
repaired precast and the undamaged specimens showed that the
repaired specimens were sufficiently strong, thus proving that repair
to damaged precast beam to column post tensioned connections can
be undertaken.
Abstract: The main aim of this research was to investigate a
prototype bamboo shading device. There were two objectives to this
study: first, to investigate the effects of non-chemical treatments on
bamboo shading devices damaged by powder-post beetles and fungi,
and second to develop a prototype bamboo shading device. This
study of the effects of non-chemical treatments on bamboo shading
devices damage by powder-post beetles in the laboratory showed
that, among seven treatments tested, wood vinegar treatment can
protect powder-post beetles better than the original method by up to
92.91%. It was also found that wood vinegar treatment shows the
best performance in fungi protection and works better than the
original method by up to 40%. A second experiment was carried out
by constructing four bamboo shading devices and installing them on
a building for 28 days. All aspects of shading device were
investigated in terms of their beauty, durability, and ease of
construction and assembly. The final prototype was developed from
the lessons learned from the test results. In conclusion, this study
showed the effectiveness of some natural preservatives against insect
and fungi damage, and it also illustrated the characteristics of a
prototype bamboo shading device that can be constructed by rural
workers within one week.
Abstract: Agriculture is the backbone of economy of Pakistan
and cotton is the major agricultural export and supreme source of raw
fiber for our textile industry. To combat severe problems of insect
and weed, combination of three genes namely Cry1Ac, Cry2A and
EPSPS genes was transferred in locally cultivated cotton variety
MNH-786 with the use of Agrobacterium mediated genetic
transformation. The present study focused on the molecular screening
of transgenic cotton plants at T3 generation in order to confirm
integration and expression of all three genes (Cry1Ac, Cry2A and
EPSP synthase) into the cotton genome. Initially, glyphosate spray
assay was used for screening of transgenic cotton plants containing
EPSP synthase gene at T3 generation. Transgenic cotton plants which
were healthy and showed no damage on leaves were selected after 07
days of spray. For molecular analysis of transgenic cotton plants in
the laboratory, the genomic DNA of these transgenic cotton plants
were isolated and subjected to amplification of the three genes. Thus,
seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A
gene) and all twenty (EPSP synthase gene) were produced positive
amplification. On the base of PCR amplification, ten transgenic plant
samples were subjected to protein expression analysis through
ELISA. The results showed that eight out of ten plants were actively
expressing the three transgenes. Real-time PCR was also done to
quantify the mRNA expression levels of Cry1Ac and EPSP synthase
gene. Finally, eight plants were confirmed for the presence and active
expression of all three genes at T3 generation.
Abstract: This study examines analytically the effect of tsunami loads on reinforced concrete (RC) frame buildings. The impact of tsunami wave loads and waterborne objects are analyzed using a typical substandard full-scale two-story RC frame building tested as part of the EU-funded Ecoleader project. The building was subjected to shake table tests in bare condition, and subsequently strengthened using Carbon Fiber Reinforced Polymers (CFRP) composites and retested. Numerical models of the building in both bare and CFRP-strengthened conditions are calibrated in DRAIN-3DX software to match the test results. To investigate the response of wave loads and impact forces, the numerical models are subjected to nonlinear dynamic analyses using force time-history input records. The analytical results are compared in terms of displacements at the floors and at the “impact point” of a boat. The results show that the roof displacement of the CFRP-strengthened building reduced by 63% when compared to the bare building. The results also indicate that strengthening only the mid-height of the impact column using CFRP is more effective at reducing damage when compared to strengthening other parts of the column. Alternative solutions to mitigate damage due to tsunami loads are suggested.
Abstract: In this investigation, we have evaluated the effects of
arsenic trioxide on hepatic function in pregnant and lactating Swiss
albino mice and their suckling pups. Experiments were carried out on
female mice given 175 ppm As2O3 in their drinking water from the
14th day of pregnancy until day 14 after delivery. Our results showed
a significant decrease in plasma levels of total protein and albumin,
cholesterol and triglyceride in As2O3 treated mice and their pups. The
hyperbilirubinemia and the increased plasma total alkaline
phosphatase activity suggested the presence of cholestasis.
Transaminase activities as well as lactate deshydrogenase activity in
plasma, known as biomarkers of hepatocellular injury, were elevated
indicating hepatic cells’ damage after treatment with As2O3.
Exposure to arsenic led to an increase of liver thiobarbituric acid
reactive substances level along with a concomitant decrease in the
activities of superoxide dismutase, catalase and glutathione
peroxidase and in glutathione.
Abstract: Although, arsenic trioxide has been the subject of
toxicological research, in vitro cytotoxicity and genotoxicity studies
using relevant cell models and uniform methodology are not well
elucidated. Hence, the aim of the present study was to evaluate the
cytotoxicity and genotoxicity induced by arsenic trioxide in human
keratinocytes (HaCaT) using the MTT [3-(4, 5-dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide] and alkaline single cell gel
electrophoresis (Comet) assays, respectively. Human keratinocytes
were treated with different doses of arsenic trioxide for 4 h prior to
cytogenetic assessment. Data obtained from the MTT assay indicated
that arsenic trioxide significantly reduced the viability of HaCaT cells
in a dose-dependent manner, showing an IC50 value of 34.18 ± 0.6
μM. Data generated from the comet assay also indicated a significant
dose-dependent increase in DNA damage in HaCaT cells associated
with arsenic trioxide exposure. We observed a significant increase in
comet tail length and tail moment, showing an evidence of arsenic
trioxide -induced genotoxic damage in HaCaT cells. This study
confirms that the comet assay is a sensitive and effective method to
detect DNA damage caused by arsenic.
Abstract: The use OF adhesive anchors for wooden constructions is an efficient technology to connect and design timber members in new timber structures and to rehabilitate the damaged structural members of historical buildings. Due to the lack of standard regulation in this specific area of structural design, designers’ choices are still supported by test analysis that enables knowledge, and the prediction, of the structural behaviour of glued in rod joints. The paper outlines an experimental research activity aimed at identifying the tensile resistance capacity of several new adhesive joint prototypes made of epoxy resin, steel bar and timber, Oak and Douglas Fir species. The development of new adhesive connectors has been carried out by using epoxy to glue stainless steel bars into pre-drilled holes, characterised by smooth and rough internal surfaces, in timber samples. The realization of a threaded contact surface using a specific drill bit has led to an improved bond between wood and epoxy. The applied changes have also reduced the cost of the joints’ production. The paper presents the results of this parametric analysis and a Finite Element analysis that enables identification and study of the internal stress distribution in the proposed adhesive anchors.
Abstract: Modern low earth orbit (LEO) satellites that require multi-mission flexibility are highly likely to be repositioned between different operational orbits. While executing this process the satellite may experience high levels of vibration and environmental hazards, exposing the deployed solar panel to dangerous stress levels, fatigue and space debris, hence it is desirable to retract the solar array before satellite repositioning to avoid damage or failure.
A novel concept of deployable/retractable hybrid solar array systemcomposed of both rigid and flexible solar panels arranged within a petal formation, aimed to provide a greater power to volume ratio while dramatically reducing mass and cost is proposed.
Abstract: Fiber Bragg optic sensor is embedded in composite
material to detect and monitor the damage that occurs in composite
structures. In this paper, we deal with the mode-Ι delamination to
determine the material strength to crack propagation, using the
coupling mode theory and T-matrix method to simulate the FBGs
spectrum for both uniform and non-uniform strain distribution. The
double cantilever beam test is modeled in FEM to determine the
longitudinal strain. Two models are implemented, the first is the
global half model, and the second is the sub-model to represent the
FBGs with higher refined mesh. This method can simulate damage in
composite structures and converting strain to a wavelength shifting in
the FBG spectrum.
Abstract: Ocimum americanum L (Lamiaceae) is an annual herb
that is native to tropical Africa. The in vitro and in vivo antioxidant
activity of its aqueous extract was carefully investigated by assessing
the DPPH radical scavenging activity, ABTS radical scavenging
activity and hydrogen peroxide radical scavenging activity. The
reducing power, total phenol, total flavonoids and flavonols content
of the extract were also evaluated. The data obtained revealed that the
extract is rich in polyphenolic compounds and scavenged the radicals
in a concentration dependent manner. This was done in comparison
with the standard antioxidants such as BHT and Vitamin C. Also, the
induction of oxidative damage with paracetamol (2000 mg/kg)
resulted in the elevation of lipid peroxides and significant (P < 0.05)
decrease in activities of superoxide dismutase, glutathione
peroxidase, glutathione reductase and catalase in the liver and kidney
of rats. However, the pretreatment of rats with aqueous extract of O.
americanum leaves (200 and 400 mg/kg) and silymarin (100 mg/kg)
caused a significant (P < 0.05) reduction in the values of lipid
peroxides and restored the levels of antioxidant parameters in these
organs. These findings suggest that the leaves of O. americanum have
potent antioxidant properties which may be responsible for its
acclaimed folkloric uses.
Abstract: Building loss estimation methodologies which have
been advanced considerably in recent decades are usually used to
estimate socio and economic impacts resulting from seismic structural
damage. In accordance with these methods, this paper presents the
evaluation of an annual loss probability of a reinforced concrete
moment resisting frame designed according to Korean Building Code.
The annual loss probability is defined by (1) a fragility curve obtained
from a capacity spectrum method which is similar to a method adopted
from HAZUS, and (2) a seismic hazard curve derived from annual
frequencies of exceedance per peak ground acceleration. Seismic
fragilities are computed to calculate the annual loss probability of a
certain structure using functions depending on structural capacity,
seismic demand, structural response and the probability of exceeding
damage state thresholds. This study carried out a nonlinear static
analysis to obtain the capacity of a RC moment resisting frame
selected as a prototype building. The analysis results show that the
probability of being extensive structural damage in the prototype
building is expected to 0.01% in a year.
Abstract: Fracture in hot precision forging of engine valves was
investigated in this paper. The entire valve forging procedure was
described and the possible cause of the fracture was proposed. Finite
Element simulation was conducted for the forging process, with
commercial Finite Element code DEFORMTM. The effects of
material properties, the effect of strain rate and temperature were
considered in the FE simulation. Two fracture criteria were discussed
and compared, based on the accuracy and reliability of the FE
simulation results. The selected criterion predicted the fracture
location and shows the trend of damage increasing with good
accuracy, which matches the experimental observation. Additional
modification of the punch shapes was proposed to further reduce the
tendency of fracture in forging. Finite Element comparison shows a
great potential of such application in the mass production.
Abstract: At certain depths during large diameter displacement
pile driving, rebound well over 0.25 inches was experienced,
followed by a small permanent-set during each hammer blow. High
pile rebound (HPR) soils may stop the pile driving and results in a
limited pile capacity. In some cases, rebound leads to pile damage,
delaying the construction project, and the requiring foundations
redesign. HPR was evaluated at seven Florida sites, during driving of
square precast, prestressed concrete piles driven into saturated, fine
silty to clayey sands and sandy clays. Pile Driving Analyzer (PDA)
deflection versus time data recorded during installation, was used to
develop correlations between cone penetrometer (CPT) pore-water
pressures, pile displacements and rebound. At five sites where piles
experienced excessive HPR with minimal set, the pore pressure
yielded very high positive values of greater than 20 tsf. However, at
the site where the pile rebounded, followed by an acceptable
permanent-set, the measured pore pressure ranged between 5 and 20
tsf. The pore pressure exhibited values of less than 5 tsf at the site
where no rebound was noticed. In summary, direct correlations
between CPTu pore pressure and rebound were produced, allowing
identification of soils that produce HPR.
Abstract: Liver disorders are one of the major problems of the
world. Despite its frequent occurrence, high morbidity and high
mortality, its medical management is currently inadequate. This study
was designed to evaluate the hepatoprotective effect of saponin
extract of the root of Garcinia kola on the integrity of the liver of
paracetamol induced wistar albino rats. Twenty five (25) male adult
wistar albino rats were divided into five (5) groups. Group I was the
Control group that received distilled water only, group II was the
negative control that received 2 g/kg of paracetamol on the 13th day,
and group III, IV and V were pre-treated with 100, 200 and
400mg/kg of the saponin extract before inducing the liver damage on
the 13th day with 2 g/kg of paracetamol. Twenty four (24) h after
administration, the rats were sacrificed and blood samples were
collected. The serum Alanine Transaminase (ALT), Aspartate
Transaminase (AST), Alkaline Phosphatase (ALP) activities,
Bilirubin and conjugated bilirubin, glucose and protein
concentrations were evaluated. The liver was fixed immediately in
Formalin and was processed and stained in Haematoxylin and Eosin
(H&E). Administration of saponin extract from the root of Garcinia
kola significantly decreased paracetamol induced elevated enzymes
in the test group. Also histological observations showed that saponin
extract of the root of Garcinia kola exhibited a significant liver
protection against the toxicant as evident by the cells trying to return
to normal. Saponin extract from the root of Garcinia kola indicated a
protection of structural integrity of the hepatocytic cell membrane
and regeneration of the damaged liver.
Abstract: A capacity spectrum method (CSM), one of methodologies to evaluate seismic fragilities of building structures, has been long recognized as the most convenient method, even if it contains several limitations to predict the seismic response of structures of interest. This paper proposes the procedure to estimate seismic fragility curves using an incremental dynamic analysis (IDA) rather than the method adopting a CSM. To achieve the research purpose, this study compares the seismic fragility curves of a 5-story reinforced concrete (RC) moment frame obtained from both methods; an IDA method and aCSM. Both seismic fragility curves are similar in slight and moderate damage states whereas the fragility curve obtained from the IDA method presents less variation (or uncertainties) in extensive and complete damage states. This is due to the fact that the IDA method can properly capture the structural response beyond yielding rather than the CSM and can directly calculate higher mode effects. From these observations, the CSM could overestimate seismic vulnerabilities of the studied structure in extensive or complete damage states.
Abstract: Over the last few decades, oilfield service rolling
equipment has significantly increased in weight, primarily because of
emissions regulations, which require larger/heavier engines, larger
cooling systems, and emissions after-treatment systems, in some
cases, etc. Larger engines cause more vibration and shock loads,
leading to failure of electronics and control systems.
If the vibrating frequency of the engine matches the system
frequency, high resonance is observed on structural parts and mounts.
One such existing automated control equipment system comprising
wire rope mounts used for mounting computers was designed
approximately 12 years ago. This includes the use of an industrialgrade
computer to control the system operation. The original
computer had a smaller, lighter enclosure. After a few years, a newer
computer version was introduced, which was 10 lbm heavier. Some
failures of internal computer parts have been documented for cases in
which the old mounts were used. Because of the added weight, there
is a possibility of having the two brackets impact each other under
off-road conditions, which causes a high shock input to the computer
parts. This added failure mode requires validating the existing mount
design to suit the new heavy-weight computer.
This paper discusses the modal finite element method (FEM)
analysis and experimental modal analysis conducted to study the
effects of vibration on the wire rope mounts and the computer. The
existing mount was modelled in ANSYS software, and resultant
mode shapes and frequencies were obtained. The experimental modal
analysis was conducted, and actual frequency responses were
observed and recorded.
Results clearly revealed that at resonance frequency, the brackets
were colliding and potentially causing damage to computer parts. To
solve this issue, spring mounts of different stiffness were modeled in
ANSYS software, and the resonant frequency was determined.
Increasing the stiffness of the system increased the resonant
frequency zone away from the frequency window at which the engine
showed heavy vibrations or resonance. After multiple iterations in
ANSYS software, the stiffness of the spring mount was finalized,
which was again experimentally validated.
Abstract: In this paper, a coupled damage effect in the
instability of a composite rotor is presented, under dynamic loading
response in the harmonic analysis condition. The analysis of the
stress which operates the rotor is done. Calculations of different
energies and the virtual work of the aerodynamic loads from the rotor
blade are developed. The use of the composite material for the rotor
offers a good stability.
Numerical calculations on the model developed prove that the
damage effect has a negative effect on the stability of the rotor.
The study of the composite rotor in transient system allowed
determining the vibratory responses due to various excitations.
Abstract: A key issue in seismic risk analysis within the context
of Performance-Based Earthquake Engineering is the evaluation of
the expected seismic damage of structures under a specific
earthquake ground motion. The assessment of the seismic
performance strongly depends on the choice of the seismic Intensity
Measure (IM), which quantifies the characteristics of a ground
motion that are important to the nonlinear structural response. Several
conventional IMs of ground motion have been used to estimate their
damage potential to structures. Yet, none of them has been proved to
be able to predict adequately the seismic damage. Therefore,
alternative, scalar intensity measures, which take into account not
only ground motion characteristics but also structural information
have been proposed. Some of these IMs are based on integration of
spectral values over a range of periods, in an attempt to account for
the information that the shape of the acceleration, velocity or
displacement spectrum provides. The adequacy of a number of these
IMs in predicting the structural damage of 3D R/C buildings is
investigated in the present paper. The investigated IMs, some of
which are structure specific and some are non structure-specific, are
defined via integration of spectral values. To achieve this purpose
three symmetric in plan R/C buildings are studied. The buildings are
subjected to 59 bidirectional earthquake ground motions. The two
horizontal accelerograms of each ground motion are applied along
the structural axes. The response is determined by nonlinear time
history analysis. The structural damage is expressed in terms of the
maximum interstory drift as well as the overall structural damage
index. The values of the aforementioned seismic damage measures
are correlated with seven scalar ground motion IMs. The comparative
assessment of the results revealed that the structure-specific IMs
present higher correlation with the seismic damage of the three
buildings. However, the adequacy of the IMs for estimation of the
structural damage depends on the response parameter adopted.
Furthermore, it was confirmed that the widely used spectral
acceleration at the fundamental period of the structure is a good
indicator of the expected earthquake damage level.
Abstract: Precast residential houses are normally constructed in Malaysia using precast shear-key wall panel and this panel is designed using BS8110 where there is no provision for earthquake. However, the safety of this house under moderate and strong earthquake is still questionable. Consequently, the full-scale of residential house are designed, constructed, tested and analyzed under in-plane lateral quasi-static cyclic loading. Hysteresis loops are plotted based on the experimental work and compared with modeling of hysteresis loops using HYSTERES in RUAUMOKO 2D program. Modified Takeda hysteresis model is chosen to behave a similar pattern with experimental work. This program will display the earthquake excitations, spectral displacements, pseudo spectral acceleration, mode shape and deformation of the structure. It can be concluded that this building is suffering severe cracks and damage under moderate and severe earthquake.