Abstract: The mechanisms underlying the association between
obesity and asthma may be related to a decreased immunological
tolerance induced by a defective function of regulatory T cells
(Tregs). The aim of this study is to establish the potential link
between these diseases and CD4+, CD25+ FoxP3+ Tregs as well as T
helper cells (Ths) in children. This is a prospective case control
study. Obese (n:40), asthmatic (n:40), asthmatic obese (n:40) and
healthy children (n:40), who don't have any acute or chronic diseases,
were included in this study. Obese children were evaluated according
to WHO criteria. Asthmatic patients were chosen based on GINA
criteria. Parents were asked to fill up the questionnaire. Informed
consent forms were taken. Blood samples were marked with CD4+,
CD25+ and FoxP3+ in order to determine Tregs and Ths by flow
cytometric method. Statistical analyses were performed. p≤0.05 was
chosen as meaningful threshold. Tregs exhibiting anti-inflammatory
nature were significantly lower in obese (0,16%; p≤0,001), asthmatic
(0,25%; p≤0,01) and asthmatic obese (0,29%; p≤0,05) groups than
the control group (0,38%). Ths were counted higher in asthma group
than the control (p≤0,01) and obese (p≤0,001) groups. T cell
immunity plays important roles in obesity and asthma pathogeneses.
Decreased numbers of Tregs found in obese, asthmatic and asthmatic
obese children may help to elucidate some questions in
pathophysiology of these diseases. For HOMA-IR levels, any
significant difference was not noted between control and obese
groups, but statistically higher values were found for obese
asthmatics. The values obtained in all groups were found to be below
the critical cut off points. This finding has made the statistically
significant difference observed between Tregs of obese, asthmatic,
obese asthmatic and control groups much more valuable. These
findings will be useful in diagnosis and treatment of these disorders
and future studies are needed. The production and propagation of
Tregs may be promising in alternative asthma and obesity treatments.
Abstract: This article focuses on upper-extremity musculoskeletal disorders risk assessment model at workplace. In this model are used risk factors that are responsible for musculoskeletal system damage. Based on statistic calculations the model is able to define what risk of MSD threatens workers who are under risk factors. The model is also able to say how MSD risk would decrease if these risk factors are eliminated.
Abstract: Image segmentation plays an important role in
medical imaging applications. Therefore, accurate methods are
needed for the successful segmentation of medical images for
diagnosis and detection of various diseases. In this paper, we have
used maximum entropy to achieve image segmentation. Maximum
entropy has been calculated using Shannon, Renyi and Tsallis
entropies. This work has novelty based on the detection of skin lesion
caused by the bite of a parasite called Sand Fly causing the disease is
called Cutaneous Leishmaniasis.
Abstract: In medical investigations, uncertainty is a major
challenging problem in making decision for doctors/experts to
identify the diseases with a common set of symptoms and also has
been extensively increasing in medical diagnosis problems. The
theory of cross entropy for intuitionistic fuzzy sets (IFS) is an
effective approach in coping uncertainty in decision making for
medical diagnosis problem. The main focus of this paper is to
propose a new intuitionistic fuzzy cross entropy measure (IFCEM),
which aid in reducing the uncertainty and doctors/experts will take
their decision easily in context of patient’s disease. It is shown that
the proposed measure has some elegant properties, which
demonstrates its potency. Further, it is also exemplified in detail the
efficiency and utility of the proposed measure by using a real life
case study of diagnosis the disease in medical science.
Abstract: Pulmonary Function Tests are important non-invasive
diagnostic tests to assess respiratory impairments and provides
quantifiable measures of lung function. Spirometry is the most
frequently used measure of lung function and plays an essential role
in the diagnosis and management of pulmonary diseases. However,
the test requires considerable patient effort and cooperation,
markedly related to the age of patients resulting in incomplete data
sets. This paper presents, a nonlinear model built using Multivariate
adaptive regression splines and Random forest regression model to
predict the missing spirometric features. Random forest based feature
selection is used to enhance both the generalization capability and the
model interpretability. In the present study, flow-volume data are
recorded for N= 198 subjects. The ranked order of feature importance
index calculated by the random forests model shows that the
spirometric features FVC, FEF25, PEF, FEF25-75, FEF50 and the
demographic parameter height are the important descriptors. A
comparison of performance assessment of both models prove that, the
prediction ability of MARS with the `top two ranked features namely
the FVC and FEF25 is higher, yielding a model fit of R2= 0.96 and
R2= 0.99 for normal and abnormal subjects. The Root Mean Square
Error analysis of the RF model and the MARS model also shows that
the latter is capable of predicting the missing values of FEV1 with a
notably lower error value of 0.0191 (normal subjects) and 0.0106
(abnormal subjects) with the aforementioned input features. It is
concluded that combining feature selection with a prediction model
provides a minimum subset of predominant features to train the
model, as well as yielding better prediction performance. This
analysis can assist clinicians with a intelligence support system in the
medical diagnosis and improvement of clinical care.
Abstract: Diminished antioxidant defense or increased
production of reactive oxygen species in the biological system can
result in oxidative stress which may lead to various
neurodegenerative diseases including Alzheimer’s disease (AD).
Microglial activation also contributes to the progression of AD by
producing several proinflammatory cytokines, nitric oxide (NO) and
prostaglandin E2 (PGE2). Oxidative stress and inflammation have
been reported to be possible pathophysiological mechanisms
underlying AD. In addition, the cholinergic hypothesis postulates that
memory impairment in patient with AD is also associated with the
deficit of cholinergic function in the brain. Although a number of
drugs have been approved for the treatment of AD, most of these
synthetic drugs have diverse side effects and yield relatively modest
benefits. Marine algae have great potential in pharmaceutical and
biomedical applications as they are valuable sources of bioactive
properties such as anticoagulation, antimicrobial, antioxidative,
anticancer and anti-inflammatory. Hence, this study aimed to provide
an overview of the properties of Malaysian seaweeds (Padina
australis, Sargassum polycystum and Caulerpa racemosa) in
inhibiting oxidative stress, neuroinflammation and cholinesterase
enzymes. These seaweeds significantly exhibited potent DPPH and
moderate superoxide anion radical scavenging ability (P
Abstract: Anxiety is a common psychological problem and also
implicated as a contributor to many chronic diseases which decreased
quality of life even with pharmacological treatment. At the present
time several yogic practices- meditation, pranayama, and mantra,
etcetera are playing important role in treating physiological and
psychological problems. Hence, the present investigation is aimed to
see the effect of Trataka on the level of anxiety among adolescents.
For the present study, a sample of 30 adolescents belonging to the
age range 20-30 years was selected from Devsanskriti Vishwa
Vidyalaya Haridwar through random sampling. In this investigation,
Sinha’s Comprehensive anxiety test has been used to measure the
level of anxiety. Statistical analysis has been done by using t-test.
Findings of this study reveal that Trataka significantly decreases the
level of anxiety among adolescents.
Abstract: For the treatment of acute and chronic lung diseases it is preferred to deliver medicaments by inhalation. The drug is delivered directly to tracheobronchial tree. This way allows the given medicament to get directly into the place of action and it makes rapid onset of action and maximum efficiency. The transport of aerosol particles in the particular part of the lung is influenced by their size, anatomy of the lungs, breathing pattern and airway resistance. This article deals with calculation of airway resistance in the lung model of Horsfield. It solves the problem of determination of the pressure losses in bifurcation and thus defines the pressure drop at a given location in the bronchial tree. The obtained data will be used as boundary conditions for transport of aerosol particles in a central part of bronchial tree realized by Computational Fluid Dynamics (CFD) approach. The results obtained from CFD simulation will allow us to provide information on the required particle size and optimal inhalation technique for particle transport into particular part of the lung.
Abstract: This paper presents an evolutionary algorithm for
solving multi-objective optimization problems-based artificial neural
network (ANN). The multi-objective evolutionary algorithm used in
this study is genetic algorithm while ANN used is radial basis
function network (RBFN). The proposed algorithm named memetic
elitist Pareto non-dominated sorting genetic algorithm-based RBFN
(MEPGAN). The proposed algorithm is implemented on medical
diseases problems. The experimental results indicate that the
proposed algorithm is viable, and provides an effective means to
design multi-objective RBFNs with good generalization capability
and compact network structure. This study shows that MEPGAN
generates RBFNs coming with an appropriate balance between
accuracy and simplicity, comparing to the other algorithms found in
literature.
Abstract: In Brazil, neonatal mortality rate is considered
incompatible with the country development conditions, and has been
a Public Health concern. Reduction in infant mortality rates has also
been part of the Millennium Development Goals, a commitment
made by countries, members of the Organization of United Nations
(OUN), including Brazil. Fetal mortality rate is considered a highly
sensitive indicator of health care quality. Suitable actions, such as
good quality and access to health services may contribute positively
towards reduction in these fetal and neonatal rates. With appropriate
antenatal follow-up and health care during gestation and delivery,
some death causes could be reduced or even prevented by means of
early diagnosis and intervention, as well as changes in risk factors
and interventions. Objectives: To study the quality of maternal and
infant health care based on fetal and neonatal mortality, as well as the
possible actions to prevent those deaths in Botucatu (Brazil).
Methods: Classification of prevention according to the International
Classification of Diseases and the modified Wigglesworth´s
classification. In order to evaluate adequacy, indicators of quality of
antenatal and delivery care were established by the authors. Results:
Considering fetal deaths, 56.7% of them occurred before delivery,
which reveals possible shortcomings in antenatal care, and 38.2% of
them were a result of intra- labor changes, which could be prevented
or reduced by adequate obstetric management. These findings were
different from those in the group of early neonatal deaths which were
also studied. Adequacy of health services showed that antenatal and
childbirth care was appropriate for 24% and 33.3% of pregnant
women, respectively, which corroborates the results of prevention.
These results revealed that shortcomings in obstetric and antenatal
care could be the causes of deaths in the study. Early and late
neonatal deaths have similar characteristics: 76% could be prevented
or reduced mainly by adequate newborn care (52.9%) and adequate
health care for gestational women (11.7%). When adequacy of care
was evaluated, childbirth and newborn care was adequate in 25.8%
and antenatal care was adequate in 16.1%. In conclusion, direct
relationship was found between adequacy and quality of care
rendered to pregnant women and newborns, and fetal and infant
mortality. Moreover, our findings highlight that deaths could be
prevented by an adequate obstetric and neonatal management.
Abstract: Compositions of different molar ratios of
polymethylmethacrylate-co-methacrylic acid (PMMA-co-MAA)
were synthesized via free-radical polymerization. Polymer coated
surfaces have been produced on silicon wafers. Coated samples were
analyzed by atomic force microscopy (AFM). The results have shown
that the roughness of the surfaces have increased by increasing the
molar ratio of monomer methacrylic acid (MAA). This study reveals
that the gradual increase in surface roughness is due to the fact that
carboxylic functional groups have been generated by MAA segments.
Such surfaces can be desirable platforms for fabrication of the
biosensors for detection of the viruses and diseases.
Abstract: It is difficult to study the effect of various variables on
cycle fitting through actual experiment. To overcome such difficulty,
the forward dynamics of a musculoskeletal model was applied to cycle
fitting in this study. The measured EMG data weres compared with the
muscle activities of the musculoskeletal model through forward
dynamics. EMG data were measured from five cyclists who do not
have musculoskeletal diseases during three minutes pedaling with a
constant load (150 W) and cadence (90 RPM). The muscles used for
the analysis were the Vastus Lateralis (VL), Tibialis Anterior (TA),
Bicep Femoris (BF), and Gastrocnemius Medial (GM). Person’s
correlation coefficients of the muscle activity patterns, the peak timing
of the maximum muscle activities, and the total muscle activities were
calculated and compared. BIKE3D model of AnyBody (Anybodytech,
Denmark) was used for the musculoskeletal model simulation. The
comparisons of the actual experiments with the simulation results
showed significant correlations in the muscle activity patterns (VL:
0.789, TA: 0.503, BF: 0.468, GM: 0.670). The peak timings of the
maximum muscle activities were distributed at particular phases. The
total muscle activities were compared with the normalized muscle
activities, and the comparison showed about 10% difference in the VL
(+10%), TA (+9.7%), and BF (+10%), excluding the GM (+29.4%).
Thus, it can be concluded that muscle activities of model &
experiment showed similar results. The results of this study indicated
that it was possible to apply the simulation of further improved
musculoskeletal model to cycle fitting.
Abstract: Musculoskeletal injuries in school children could be
reduced improving trunk strength and hamstring flexibility. Low
levels of trunk muscle strength and hamstring flexibility may result in
acute and musculoskeletal chronic diseases. The Pilates Method can
be appropriate to improve these physical condition attributes and has
been rarely employed by this social group. On the other hand, it has
been shown that trunk strength and flexibility are different between
genders, but there is no evidence about the effect of exercise
programs designed to improve both items in school children.
Therefore the objective of this study was to measure the effect of a
six-week Pilates-based exercise program in 14 year old school
children trunk strength and hamstring flexibility, establishing
differences in gender. The sample was composed of 57 students
divided into experimental group (EG; n=30) and control group (CG;
n=27). Bench Trunk Curl test (BTC), Sörensen test and Toe-touch
test (TT) were used to measure dynamic muscular resistance in trunk
flexion, isometric strength in trunk extension and hamstring
flexibility, respectively. EG utilized the Pilates exercise program
during six-weeks (2 days/week, 55minutes/session). After this period
of training, EG improved trunk strength and hamstring flexibility
significantly but there were no significant differences within CG.
Although boys were better in BTC test and girls were better in TT
test, there were no significant differences between them.
Abstract: Food contamination occurs during post process
handling. This leads to spoilage and growth of pathogenic
microorganisms in the food, thereby reducing its shelf life or
spreading of food borne diseases. Several methods are tried and one
of which is use of antimicrobial packaging. Here, papain, a protease
enzyme, is covalently immobilized with the help of glutarldehyde on
polyurethane and used as a food wrap to protect food from microbial
contamination. Covalent immobilization of papain was achieved at a
pH of 7.4; temperature of 4°C; glutaraldehyde concentration of 0.5%;
incubation time of 24h; and 50mg of papain. The formation of -C=Nobserved
in the Fourier transform infrared spectrum confirmed the
immobilization of the enzyme on the polymer. Immobilized enzyme
retained higher activity than the native free enzyme. The modified
polyurethane showed better reduction of Staphylococcus aureus
biofilm than bare polymer film (eight folds reduction in live colonies,
two times reduction in protein and 6 times reduction in
carbohydrates). The efficacy of this was studied by wrapping it over
S. aureus contaminated cottage cheese (paneer) and cheese and
stored at a temperature of 4°C for 7days. The modified film reduced
the bacterial contamination by eight folds when compared to the bare
film. FTIR also indicated reduction in lipids, sugars and proteins in
the biofilm.
Abstract: Curcuma longa L. (Zingiberaceae), commonly known
as turmeric, has a long history of traditional uses for culinary
purposes as a spice and a food colorant. The present study aimed to
document the ethnobotanical knowledge about Curcuma longa, and
to assess the variation in the herbalists’ experience in Northeastern
Algeria. Data were collected using semi-structured questionnaires
and direct interviews with 30 herbalists. Ethnobotanical indices,
including the fidelity level (FL%), the relative frequency citation
(RFC), and use value (UV) were determined by quantitative methods.
Diversity in the level of knowledge was analyzed using univariate,
non-parametric, and multivariate statistical methods. Three main
categories of uses were recorded for C. longa: for food, for medicine,
and for cosmetic purposes. As a medicine, turmeric was used for the
treatment of gastrointestinal, dermatological, and hepatic diseases.
Medicinal and food uses were correlated with both forms of
preparation (rhizome and powder). The age group did not influence
the use. Multivariate analyses showed a significant variation in
traditional knowledge, associated with the use value, origin, quality,
and efficacy of the drug. The findings suggested that the geographical
origin of C. longa affected the use in Algeria.
Abstract: The handwriting is a physical demonstration of a
complex cognitive process learnt by man since his childhood. People
with disabilities or suffering from various neurological diseases are
facing so many difficulties resulting from problems located at the
muscle stimuli (EMG) or signals from the brain (EEG) and which
arise at the stage of writing. The handwriting velocity of the same
writer or different writers varies according to different criteria: age,
attitude, mood, writing surface, etc. Therefore, it is interesting to
reconstruct an experimental basis records taking, as primary
reference, the writing speed for different writers which would allow
studying the global system during handwriting process. This paper
deals with a new approach of the handwriting system modeling based
on the velocity criterion through the concepts of artificial neural
networks, precisely the Radial Basis Functions (RBF) neural
networks. The obtained simulation results show a satisfactory
agreement between responses of the developed neural model and the
experimental data for various letters and forms then the efficiency of
the proposed approaches.
Abstract: Kigelia africana (Lam.) Benth. (Bignoniaceae) is a
reputed traditional remedy for various human ailments such as skin
diseases, microbial infections, melanoma, stomach troubles,
metabolic disorders, malaria and general pains. In spite of the fruit
being widely used for purposes related to its antibacterial and
antifungal properties, the chemical constituents associated with the
activity have not been fully identified. To elucidate the active
principles, we evaluated the antimicrobial activity of fruit extracts
and purified fractions against Staphylococcus aureus, Enterococcus
faecalis, Moraxella catarrhalis, Escherichia coli, Candida albicans
and Candida tropicalis. Shade-dried fruits were powdered and
extracted with hydroalcoholic (1:1) mixture by soaking at room
temperature for 72 h. The crude extract was further fractionated by
column chromatography, with successive elution using hexane,
dichloromethane, ethyl acetate, acetone and methanol. The
dichloromethane and ethyl acetate fractions were combined and
subjected to column chromatography to furnish a wax and oil from
the eluates of 20% and 40% ethyl acetate in hexane, respectively. The
GC-MS and GC×GC-MS results revealed that linoleic acid, linolenic
acid, palmitic acid, arachidic acid and stearic acid were the major
constituents in both oil and wax. The crude hydroalcoholic extract
exhibited the strongest activity with MICs of 0.125-0.5 mg/mL,
followed by the ethyl acetate (MICs = 0.125-1.0 mg/mL),
dichloromethane (MICs = 0.250-2.0 mg/mL), hexane (MICs = 0.25-
2.0 mg/mL), acetone (MICs = 0.5-2.0 mg/mL) and methanol (MICs =
1.0-2.0 mg/mL), whereas the wax (MICs = 2.0-4.0 mg/mL) and oil
(MICs = 4.0-8.0 mg/mL) showed poor activity. The study concludes
that synergistic interactions of chemical constituents could be
responsible for the antimicrobial activity of K. africana fruits, which
needs a more holistic approach to understand the mechanism of its
antimicrobial activity.
Abstract: Ficus deltoidea from the Moraceae family is a popular
medicinal herb in Malaysia. It possesses strong antioxidant and antiinflammatory
properties. In the present study, the anti-inflammatory
effects of F. deltoidea extract on UVB-irradiated HaCaT
Keratinocytes were investigated. HaCaT Keratinocytes were UVBirradiated
(12.5 mJ/cm3) and were treated with 0.05, 0.08 or 0.1% of
F. deltoidea extract. Cell viability following UVB irradiation was
significantly higher in the groups treated with the F. deltoidea extract
at doses of 0.05, 0.08 or 0.1% than in control group with UVB
irradiation only. Tumor necrosis factor-α (TNF-α), interleukin-1α
(IL-1α), interleukin-6 (IL-6) and cyclooxygenase (COX-2) play
primary roles in the inflammation process upon UV irradiation and
are known to be stimulated by UVB irradiation. Treatment with the
F. deltoidea extract dramatically inhibited the UV-induced TNF-α,
IL-1α, IL-6, and COX-2 expression. These results suggest that the F.
deltoidea extract inhibits the production of pro-inflammatory
cytokines and may be an effective protective agent for the treatment
of skin diseases.
Abstract: The article demonstrates on a case study how it is
possible to identify MSD risk. It is based on a dissertation Risk
identification model of occupational diseases formation in relation to
the work activity that determines what risk can endanger workers who
are exposed to the specific risk factors. It is evaluated based on
statistical calculations. These risk factors are main cause of upperextremities
musculoskeletal disorders.
Abstract: Pomegranate (Punica granatum L.) is an ancient fruit of great medical interest and rich source of antioxidants. Pesticides as dimethoate play a crucial role in the occurrence many diseases in plants, animal and human. Therefore the ability of Pomegranate (Punica granatum L.) to alleviate hepatotoxicity induced by organophosphate pesticide dimethoate was investigated. Albino male rats were divided randomly into 4 groups and kept at 7 animals per group in an environmentally controlled condition for 6 weeks. The first group was served as a control group (basal diet), the second group fed on basal diet supplemented with 5% freeze dried pomegranate seeds, the third group fed on 20 ppm dimethoate contaminated diet and the last group fed on dimethoate contaminated diet supplemented with 5% freeze dried pomegranate seeds. The results revealed that administration of dimethoate caused high significant increased in liver functions: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities as well as lipid peroxide (malonaldhyde, MDA); on the other hand high significant decreased on glutathione (GSH), glutathione peroxidase (GPx), albumin and total protein were observed. However addition of 5% freeze dried pomegranate seeds significantly improved all previously mentioned parameters. These results indicate the dimethoate induced hepatotoxicity and highlight the protective effect of pomegranate seeds as a potential protective agent against dimethoate induced hepatotoxicity. This may be attributed to the powerful antioxidants (polyphenols, total phenols, and total flavonoids) which present in high levels in pomegranate as well as improving the immunity by activation of antioxidant enzymes GSH and GPx.