Abstract: Staphylococcus aureus, one of the microflora in a
human external auditory canal (EAC) is frequently exposed to highfrequency
electromagnetic field (HF-EMF) generated by mobile
phones. It is normally non-pathogenic but in certain circumstances, it
can cause infections. This study investigates the changes in the
physiology of S. aureus when exposed to HF-EMF of a mobile
phone. Exponentially grown S. aureus were exposed to two
conditions of EMF irradiation (standby-mode and on-call mode) at
four durations; 15, 30, 45 and 60 min. Changes in the viability and
biofilm production of the S. aureus were compared between the two
conditions of exposure. EMF from the standby-mode has enhanced
the growth of S. aureus but during on-call, the growth was
suppressed. No significant difference in the amount of biofilm
produced in both modes of exposure was observed. Thus, HF-EMF
of mobile phone affects the viability of S. aureus but not its ability to
produce biofilm.
Abstract: Multidrug resistant organisms have been taunting the
medical world for the last few decades. Even with new antibiotics
developed, resistant strains have emerged soon after. With the
advancement of nanotechnology, we investigated colloidal silver
nanoparticles for its antimicrobial activity against Pseudomonas
aeruginosa. This organism is a multidrug resistant which contributes
to the high morbidity and mortality in immunocompromised patients.
Five multidrug resistant strains were used in this study. The
antimicrobial effect was studied using the disc diffusion and broth
dilution techniques. An inhibition zone of 11 mm was observed with
10 μg dose of the nanoparticles. The nanoparticles exhibited MIC of
50 μg/ml when added at the lag phase and the subinhibitory
concentration was measured as 100 μg/ml. The MIC50 value showed
to be 15 μg/ml. This study suggests that silver nanoparticles can be
further developed as an antimicrobial agent, hence decreasing the
burden of the multidrug resistance phenomena.