Antibacterial Effect of Silver Nanoparticles on Multi Drug Resistant Pseudomonas Aeruginosa
Multidrug resistant organisms have been taunting the
medical world for the last few decades. Even with new antibiotics
developed, resistant strains have emerged soon after. With the
advancement of nanotechnology, we investigated colloidal silver
nanoparticles for its antimicrobial activity against Pseudomonas
aeruginosa. This organism is a multidrug resistant which contributes
to the high morbidity and mortality in immunocompromised patients.
Five multidrug resistant strains were used in this study. The
antimicrobial effect was studied using the disc diffusion and broth
dilution techniques. An inhibition zone of 11 mm was observed with
10 μg dose of the nanoparticles. The nanoparticles exhibited MIC of
50 μg/ml when added at the lag phase and the subinhibitory
concentration was measured as 100 μg/ml. The MIC50 value showed
to be 15 μg/ml. This study suggests that silver nanoparticles can be
further developed as an antimicrobial agent, hence decreasing the
burden of the multidrug resistance phenomena.
[1] Goossens, H. (2003). "Susceptibility of multi-drug-resistant
Pseudomonas aeruginosa in intensive care units: results from the
European MYSTIC study groupÔÇá." Clinical Microbiology and Infection
9(9): 980-983.
[2] Kerr, K. G. and A. M. Snelling (2009). "Pseudomonas aeruginosa: a
formidable and ever-present adversary." Journal of Hospital Infection
73(4): 338-344.
[3] Foran, S. M. (2009). Therapeutic Properties of Silver : An Historical
and Technical Review. Quanta.
[4] Mudshinge, S. R., A. B. Deore, et al. (2011). "Nanoparticles: Emerging
carriers for drug delivery." Saudi Pharmaceutical Journal 19(3): 129-
141.
[5] Kim, J. S., E. Kuk, et al. (2007). "Antimicrobial effects of silver
nanoparticles." Nanomedicine: Nanotechnology, Biology and Medicine
3(1): 95-101.
[6] Bhupendra Chudasama, A. K. V., Nidhi Andhariya, R. V. Metha, R. V.
Upadhyay (2010). "Highly bacterial resistant silver nanoparticles :
synthesis and antibacterial activities."
[7] Shahverdi, A. R., A. Fakhimi, et al. (2007). "Synthesis and effect of
silver nanoparticles on the antibacterial activity of different antibiotics
against Staphylococcus aureus and Escherichia coli." Nanomedicine:
Nanotechnology, Biology and Medicine 3(2): 168-171.
[8] Clinical and Laboratory Standards Institute (2000): Performance
standards for antimicrobial susceptibility testing (Wayne, P.A) 1995:
15th informational supplement. Document M100- S15.
[9] Ivan Sondi, B. S.-S. (2004). "Silver nanoparticles as antimicrobial agent
: a case study on E. coli as a model for Gram-negative bacteria." Journal
of Colloid and Interface Science: 177 - 182.
[10] Lisa A. Spacek, M. D., Ph.D.; Khalil G. Ghanem, M.D. (2011).
Pseudomonas aeruginosa, Unbound Medicine.
[11] Yamanaka, M., K. Hara, et al. (2005). "Bactericidal actions of a silver
ion solution on Escherichia coli, studied by energy-filtering
transmission electron microscopy and proteomic analysis." Applied and
environmental microbiology 71(11): 7589.
[12] Batarseh, K. I. (2004). "Anomaly and correlation of killing in the
therapeutic properties of silver (I) chelation with glutamic and tartaric
acids." Journal of Antimicrobial Chemotherapy 54(2): 546-548.
[1] Goossens, H. (2003). "Susceptibility of multi-drug-resistant
Pseudomonas aeruginosa in intensive care units: results from the
European MYSTIC study groupÔÇá." Clinical Microbiology and Infection
9(9): 980-983.
[2] Kerr, K. G. and A. M. Snelling (2009). "Pseudomonas aeruginosa: a
formidable and ever-present adversary." Journal of Hospital Infection
73(4): 338-344.
[3] Foran, S. M. (2009). Therapeutic Properties of Silver : An Historical
and Technical Review. Quanta.
[4] Mudshinge, S. R., A. B. Deore, et al. (2011). "Nanoparticles: Emerging
carriers for drug delivery." Saudi Pharmaceutical Journal 19(3): 129-
141.
[5] Kim, J. S., E. Kuk, et al. (2007). "Antimicrobial effects of silver
nanoparticles." Nanomedicine: Nanotechnology, Biology and Medicine
3(1): 95-101.
[6] Bhupendra Chudasama, A. K. V., Nidhi Andhariya, R. V. Metha, R. V.
Upadhyay (2010). "Highly bacterial resistant silver nanoparticles :
synthesis and antibacterial activities."
[7] Shahverdi, A. R., A. Fakhimi, et al. (2007). "Synthesis and effect of
silver nanoparticles on the antibacterial activity of different antibiotics
against Staphylococcus aureus and Escherichia coli." Nanomedicine:
Nanotechnology, Biology and Medicine 3(2): 168-171.
[8] Clinical and Laboratory Standards Institute (2000): Performance
standards for antimicrobial susceptibility testing (Wayne, P.A) 1995:
15th informational supplement. Document M100- S15.
[9] Ivan Sondi, B. S.-S. (2004). "Silver nanoparticles as antimicrobial agent
: a case study on E. coli as a model for Gram-negative bacteria." Journal
of Colloid and Interface Science: 177 - 182.
[10] Lisa A. Spacek, M. D., Ph.D.; Khalil G. Ghanem, M.D. (2011).
Pseudomonas aeruginosa, Unbound Medicine.
[11] Yamanaka, M., K. Hara, et al. (2005). "Bactericidal actions of a silver
ion solution on Escherichia coli, studied by energy-filtering
transmission electron microscopy and proteomic analysis." Applied and
environmental microbiology 71(11): 7589.
[12] Batarseh, K. I. (2004). "Anomaly and correlation of killing in the
therapeutic properties of silver (I) chelation with glutamic and tartaric
acids." Journal of Antimicrobial Chemotherapy 54(2): 546-548.
@article{"International Journal of Medical, Medicine and Health Sciences:50197", author = "Athirah Nur Amirulhusni and Navindra Kumari Palanisamy and Zaini Mohd-Zain and Liew Jian Ping and R.Durairaj", title = "Antibacterial Effect of Silver Nanoparticles on Multi Drug Resistant Pseudomonas Aeruginosa", abstract = "Multidrug resistant organisms have been taunting the
medical world for the last few decades. Even with new antibiotics
developed, resistant strains have emerged soon after. With the
advancement of nanotechnology, we investigated colloidal silver
nanoparticles for its antimicrobial activity against Pseudomonas
aeruginosa. This organism is a multidrug resistant which contributes
to the high morbidity and mortality in immunocompromised patients.
Five multidrug resistant strains were used in this study. The
antimicrobial effect was studied using the disc diffusion and broth
dilution techniques. An inhibition zone of 11 mm was observed with
10 μg dose of the nanoparticles. The nanoparticles exhibited MIC of
50 μg/ml when added at the lag phase and the subinhibitory
concentration was measured as 100 μg/ml. The MIC50 value showed
to be 15 μg/ml. This study suggests that silver nanoparticles can be
further developed as an antimicrobial agent, hence decreasing the
burden of the multidrug resistance phenomena.", keywords = "Antimirobial activity, Multidrug resistance,
Pseudomonas aeruginosa, Silver nanoparticles", volume = "6", number = "7", pages = "292-4", }