Abstract: Lawsone is a pigment that occurs naturally in plants.
It has been used as a skin and hair dye for a long time. Moreover, its
different biological activities have been reported. The present study
focused on the effect of lawsone on a plant cell model represented by
tobacco BY-2 cell suspension culture, which is used as a model
comparable with the HeLa cells. It has been shown that lawsone
inhibits the cell growth in the concentration-dependent manner. In
addition, changes in DNA methylation level have been determined.
We observed decreasing level of DNA methylation in the presence of
increasing concentrations of lawsone. These results were
accompanied with overproduction of reactive oxygen species (ROS).
Since epigenetic modifications can be caused by different stress
factors, there could be a connection between the changes in the level
of DNA methylation and ROS production caused by lawsone.
Abstract: Here we report on the utilization of Laser-Induced
Breakdown Spectroscopy (LIBS) for determination of Quantum Dots
(QDs) in liquid solution. The process of optimization of experimental
conditions from choosing the carrier medium to application of colloid
QDs is described. The main goal was to get the best possible signal to
noise ratio.
The results obtained from the measurements confirmed the capability
of LIBS technique for qualitative and afterwards quantitative
determination of QDs in liquid solution.