Lawsone is a pigment that occurs naturally in plants.
It has been used as a skin and hair dye for a long time. Moreover, its
different biological activities have been reported. The present study
focused on the effect of lawsone on a plant cell model represented by
tobacco BY-2 cell suspension culture, which is used as a model
comparable with the HeLa cells. It has been shown that lawsone
inhibits the cell growth in the concentration-dependent manner. In
addition, changes in DNA methylation level have been determined.
We observed decreasing level of DNA methylation in the presence of
increasing concentrations of lawsone. These results were
accompanied with overproduction of reactive oxygen species (ROS).
Since epigenetic modifications can be caused by different stress
factors, there could be a connection between the changes in the level
of DNA methylation and ROS production caused by lawsone.
[1] P. Babula, R. Mikelova, V. Adam, D. Potesil, J. Zehnalek, R. Kizek, L. Havel, and Z. Sladky, "Chromatographic analysis of naphthoquinones in plants,” Chem. Listy, vol. 100, pp. 271-276, Jan. 2006.
[2] P. Babula, V. Adam, L. Havel, and R. Kizek, "Noteworthy secondary metabolites naphthoquinones – their occurrence, pharmacological properties and analysis,” Curr. Pharm. Anal., vol. 5, no. 1, pp. 47-68, Jan. 2009.
[3] P. Babula, V. Adam, R. Kizek, Z. Sladky, and L. Havel, "Naphthoquinones as allelochemical triggers of programmed cell death,” Environ. Exp. Bot., vol. 65, pp. 330-337, March 2009.
[4] S. M. G. Saeed, S. A.Sayeed, S. Ashraf, S. Naz, R. Siddiqi, R. Ali, and M. A. Mesaik, ”A new method for the isolation and purification of lawsone from Lawsoniainermis and its ROS inhibitory activity,” Pak. J. Bot., vol. 45, no. 4, pp. 1431-1436, Aug. 2013.
[5] R. Sauriasari, D. H. Wang, Y. Takemura, K. Tsutsui, N. Masuoka, K. Sano, M. Horita, B. L. Wang, and K. Oqino, "Cytotoxicity of lawsone and cytoprotectiveactivity of antioxidants in catalase mutant Escherichia coli,”Toxicology, vol. 235, no. 1-2, pp. 103-111, Jun. 2007.
[6] H. Sano, "Inheritance of acquired traits in plants. Reinstatement of Lamarck.” Plant Signal. Behav., vol. 5, no. 4, pp. 346-348, Apr. 2010.
[7] H. H. Ng, and A. Bird, "DNA methylation and chromatin modification,” Curr. Opin. Genet. Dev., vol. 9, no. 2, pp. 158-163, Apr. 1999.
[8] R. A. Martienssen, and V. Colot, "DNA methylation and epigenetic inheritance in plants and filamentous fungi,”Science, vol. 293, no. 5532, pp. 1070-1074, Aug. 2001.
[9] T. Murashige, and F. Skoog, "A revised medium for rapid growth and bioassays with tobacco tissue cultures,” Phys. Plant Pathol., vol. 15, no. 3, pp. 473-497, July 1962.
[10] T. Nagata, Y. Nemoto, and S. Hasezawa, "Tobacco BY-2 cell-line as the HeLa-cell in the cell biology of higher plants,” Int. Rev. Cytol., vol. 132,pp. 1-30, 1992.
[11] M. Karimi, S. Johansson, D. Stach, M. Corcoran, D. Grandér, M. Schalling, G. Bakalkin, F. Lyko, C. Larsson, and T. J. Ekström, "LUMA (LUminometric Methylation Assay)-A high throughput method to the analysis of genomic DNA methylation,” Exp. Cell Res., vol. 312, no. 11, pp. 1989-1995, July 2006.
[12] F. Q. Zhang, Y. S. Wang, Z. P. Lou, and J. D. Dong, "Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandeliacandel and Bruguieragymnorrhiza),” Chemosphere, vol. 67, no. 1, pp. 44-50, Feb. 2007.
[13] S. Oramas-Royo, C. Torrejón, I. Cuadrado, R. Hernández-Molina, S. Hortelano, A. Estévez-Braun, and B. de lasHeras, "Synthesis and cytotoxic activity of metallic complexes of lawsone,” Bioorg. Med. Chem., vol. 21, no. 9, pp. 2471-2477, May 2013.
[14] A. Esteves-Souza, D. V. Fiqueiredo, A. Esteves, C. A. Camara, M. D. Vargas, A. C. Pinto, and A. Echevarria, "Cytotoxic and DNA-topoisomerase effects of lapachol amine derivatives and interactions with DNA,” Braz. J. Med. Biol. Res., vol. 40, no. 10, pp. 1399-1402, Oct. 2007.
[15] S. A. Tammen, S. Friso, and S. W. Choi, "Epigenetics: The link between nature and nurture,” Mol. Aspects Med., vol. 34, no. 4, pp. 753-764, Jul.-Aug. 2013.
[16] A. Razin, and H. Cedar, "DNA methylation and gene expression,” Microbiol. Mol. Biol. Rev., vol. 55, no. 3, pp. 451-458, Sep. 1991.
[17] M. Tariq, and J. Paszkowski, "DNA and histone methylation in plants,” Trends Genet., vol. 20, no. 6, pp. 244-251, Jun. 2004.
[1] P. Babula, R. Mikelova, V. Adam, D. Potesil, J. Zehnalek, R. Kizek, L. Havel, and Z. Sladky, "Chromatographic analysis of naphthoquinones in plants,” Chem. Listy, vol. 100, pp. 271-276, Jan. 2006.
[2] P. Babula, V. Adam, L. Havel, and R. Kizek, "Noteworthy secondary metabolites naphthoquinones – their occurrence, pharmacological properties and analysis,” Curr. Pharm. Anal., vol. 5, no. 1, pp. 47-68, Jan. 2009.
[3] P. Babula, V. Adam, R. Kizek, Z. Sladky, and L. Havel, "Naphthoquinones as allelochemical triggers of programmed cell death,” Environ. Exp. Bot., vol. 65, pp. 330-337, March 2009.
[4] S. M. G. Saeed, S. A.Sayeed, S. Ashraf, S. Naz, R. Siddiqi, R. Ali, and M. A. Mesaik, ”A new method for the isolation and purification of lawsone from Lawsoniainermis and its ROS inhibitory activity,” Pak. J. Bot., vol. 45, no. 4, pp. 1431-1436, Aug. 2013.
[5] R. Sauriasari, D. H. Wang, Y. Takemura, K. Tsutsui, N. Masuoka, K. Sano, M. Horita, B. L. Wang, and K. Oqino, "Cytotoxicity of lawsone and cytoprotectiveactivity of antioxidants in catalase mutant Escherichia coli,”Toxicology, vol. 235, no. 1-2, pp. 103-111, Jun. 2007.
[6] H. Sano, "Inheritance of acquired traits in plants. Reinstatement of Lamarck.” Plant Signal. Behav., vol. 5, no. 4, pp. 346-348, Apr. 2010.
[7] H. H. Ng, and A. Bird, "DNA methylation and chromatin modification,” Curr. Opin. Genet. Dev., vol. 9, no. 2, pp. 158-163, Apr. 1999.
[8] R. A. Martienssen, and V. Colot, "DNA methylation and epigenetic inheritance in plants and filamentous fungi,”Science, vol. 293, no. 5532, pp. 1070-1074, Aug. 2001.
[9] T. Murashige, and F. Skoog, "A revised medium for rapid growth and bioassays with tobacco tissue cultures,” Phys. Plant Pathol., vol. 15, no. 3, pp. 473-497, July 1962.
[10] T. Nagata, Y. Nemoto, and S. Hasezawa, "Tobacco BY-2 cell-line as the HeLa-cell in the cell biology of higher plants,” Int. Rev. Cytol., vol. 132,pp. 1-30, 1992.
[11] M. Karimi, S. Johansson, D. Stach, M. Corcoran, D. Grandér, M. Schalling, G. Bakalkin, F. Lyko, C. Larsson, and T. J. Ekström, "LUMA (LUminometric Methylation Assay)-A high throughput method to the analysis of genomic DNA methylation,” Exp. Cell Res., vol. 312, no. 11, pp. 1989-1995, July 2006.
[12] F. Q. Zhang, Y. S. Wang, Z. P. Lou, and J. D. Dong, "Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandeliacandel and Bruguieragymnorrhiza),” Chemosphere, vol. 67, no. 1, pp. 44-50, Feb. 2007.
[13] S. Oramas-Royo, C. Torrejón, I. Cuadrado, R. Hernández-Molina, S. Hortelano, A. Estévez-Braun, and B. de lasHeras, "Synthesis and cytotoxic activity of metallic complexes of lawsone,” Bioorg. Med. Chem., vol. 21, no. 9, pp. 2471-2477, May 2013.
[14] A. Esteves-Souza, D. V. Fiqueiredo, A. Esteves, C. A. Camara, M. D. Vargas, A. C. Pinto, and A. Echevarria, "Cytotoxic and DNA-topoisomerase effects of lapachol amine derivatives and interactions with DNA,” Braz. J. Med. Biol. Res., vol. 40, no. 10, pp. 1399-1402, Oct. 2007.
[15] S. A. Tammen, S. Friso, and S. W. Choi, "Epigenetics: The link between nature and nurture,” Mol. Aspects Med., vol. 34, no. 4, pp. 753-764, Jul.-Aug. 2013.
[16] A. Razin, and H. Cedar, "DNA methylation and gene expression,” Microbiol. Mol. Biol. Rev., vol. 55, no. 3, pp. 451-458, Sep. 1991.
[17] M. Tariq, and J. Paszkowski, "DNA and histone methylation in plants,” Trends Genet., vol. 20, no. 6, pp. 244-251, Jun. 2004.
@article{"International Journal of Biological, Life and Agricultural Sciences:67659", author = "Zuzana Poborilova and Anna B. Ohlsson and Torkel Berglund and Anna Vildova and Petr Babula", title = "DNA Methylation Changes Caused by Lawsone", abstract = "Lawsone is a pigment that occurs naturally in plants.
It has been used as a skin and hair dye for a long time. Moreover, its
different biological activities have been reported. The present study
focused on the effect of lawsone on a plant cell model represented by
tobacco BY-2 cell suspension culture, which is used as a model
comparable with the HeLa cells. It has been shown that lawsone
inhibits the cell growth in the concentration-dependent manner. In
addition, changes in DNA methylation level have been determined.
We observed decreasing level of DNA methylation in the presence of
increasing concentrations of lawsone. These results were
accompanied with overproduction of reactive oxygen species (ROS).
Since epigenetic modifications can be caused by different stress
factors, there could be a connection between the changes in the level
of DNA methylation and ROS production caused by lawsone.
", keywords = "DNA methylation, Lawsone, Naphthoquinone,
Reactive Oxygen Species.", volume = "8", number = "7", pages = "699-4", }
