Abstract: A low-cost paper-based microfluidic device (PAD) for the multiplex electrochemical determination of glucose, uric acid, and dopamine in biological fluids was developed. Using wax printing, PAD containing a central zone, six channels, and six detection zones was fabricated, and the electrodes were printed on detection zones using pre-made electrodes template. For each analyte, two detection zones were used. The carbon working electrode was coated with chitosan-BSA (and enzymes for glucose and uric acid). To detect glucose and uric acid, enzymatic reactions were employed. These reactions involve enzyme-catalyzed redox reactions of the analytes and produce free electrons for electrochemical measurement. Calibration curves were linear (R² > 0.980) in the range of 0-80 mM for glucose, 0.09–0.9 mM for dopamine, and 0–50 mM for uric acid, respectively. Blood samples were successfully analyzed by the proposed method.
Abstract: The fatigue life of tubular joints commonly found in
offshore industry is not only dependent on the value of hot-spot stress
(HSS), but is also significantly influenced by the through-thethickness
stress distribution characterized by the degree of bending
(DoB). The determination of DoB values in a tubular joint is essential
for improving the accuracy of fatigue life estimation using the stresslife
(S–N) method and particularly for predicting the fatigue crack
growth based on the fracture mechanics (FM) approach. In the
present paper, data extracted from finite element (FE) analyses of
tubular KT-joints, verified against experimental data and parametric
equations, was used to investigate the effects of geometrical
parameters on DoB values at the crown 0°, saddle, and crown 180°
positions along the weld toe of central brace in tubular KT-joints
subjected to axial loading. Parametric study was followed by a set of
nonlinear regression analyses to derive DoB parametric formulas for
the fatigue analysis of KT-joints under axial loads. The tubular KTjoint
is a quite common joint type found in steel offshore structures.
However, despite the crucial role of the DoB in evaluating the fatigue
performance of tubular joints, this paper is the first attempt to study
and formulate the DoB values in KT-joints.
Abstract: In the present paper, a set of parametric FE stress
analyses is carried out for two-planar welded tubular DKT-joints
under two different axial load cases. Analysis results are used to
present general remarks on the effect of geometrical parameters on
the stress concentration factors (SCFs) at the inner saddle, outer
saddle, toe, and heel positions on the main (outer) brace. Then a new
set of SCF parametric equations is developed through nonlinear
regression analysis for the fatigue design of two-planar DKT-joints.
An assessment study of these equations is conducted against the
experimental data; and the satisfaction of the criteria regarding the
acceptance of parametric equations is checked. Significant effort has
been devoted by researchers to the study of SCFs in various uniplanar
tubular connections. Nevertheless, for multi-planar joints
covering the majority of practical applications, very few
investigations have been reported due to the complexity and high
cost involved.