Abstract: Honeys are produced by Apis mellifera and stingless
bees (Meliponini) in Ecuador. We studied honey produced in
beeswax combs by Apis mellifera, and honey produced in pots by
Geotrigona and Scaptotrigona bees. Chloroform extracts of honey
were obtained for fast NMR spectra. The 1D spectra were acquired at
298 K, with a 600 MHz NMR Bruker instrument, using a modified
double pulsed field gradient spin echoes (DPFGSE) sequence.
Signals of 1H NMR spectra were integrated and used as inputs for
PCA, PLS-DA analysis, and labelled sets of classes were successfully
identified, enhancing the separation between the three groups of
honey according to the entomological origin: A. mellifera,
Geotrigona and Scaptotrigona. This procedure is therefore
recommended for authenticity test of honey in Ecuador.