Abstract: Protorhus longifolia is known as a medicinal plant that has been used traditionally to treat various ailments such as hemiplegic paralysis, blood clotting related diseases, diarrhoea, heartburn, etc. The study reports a High-Performance Thin Layer Chromatography (HPTLC) fingerprint profile of Protorhus longifolia methanolic extract and its qualitative analysis of gallic acid, rutin, and quercetin. HPTLC analysis was achieved using CAMAG HPTLC system equipped with CAMAG automatic TLC sampler 4, CAMAG Automatic Developing Chamber 2 (ADC2), CAMAG visualizer 2, CAMAG Thin Layer Chromatography (TLC) scanner and visionCATS CAMAG HPTLC software. Mobile phase comprising toluene, ethyl acetate, formic acid (21:15:3) was used for qualitative analysis of gallic acid and revealed eight peaks while the mobile phase containing ethyl acetate, water, glacial acetic acid, formic acid (100:26:11:11) for qualitative analysis of rutin and quercetin revealed six peaks. HPTLC sillica gel 60 F254 glass plates (10 × 10) were used as the stationary phase. Gallic acid was detected at the Rf = 0.35; while rutin and quercetin were not evident in the extract. Further studies will be performed to quantify gallic acid in Protorhus longifolia leaves and also identify other biomarkers.
Abstract: The current web has become a modern encyclopedia,
where people share their thoughts and ideas on various topics around
them. This kind of encyclopedia is very useful for other people who
are looking for answers to their questions. However, with the
growing popularity of social networking and blogging and ever
expanding network services, there has also been a growing diversity
of technologies along with a different structure of individual web
sites. It is therefore difficult to directly find a relevant answer for a
common Internet user. This paper presents a web application for the
real-time end-to-end analysis of selected Internet trends where the
trend can be whatever the people post online. The application
integrates fully configurable tools for data collection and analysis
using selected webometric algorithms, and for its chronological
visualization to user. It can be assumed that the application facilitates
the users to evaluate the quality of various products that are
mentioned online.