Abstract: Heavy metals have bad effects on environment and
soils and it can uptake by natural HAP .natural Hap is an inexpensive
material that uptake large amounts of various heavy metals like Zn
(II) .Natural HAP (N-HAP), extracted from bovine cortical bone ash,
is a good choice for substitution of commercial HAP. Several
experiments were done to investigate the sorption capacity of Zn (II)
to N-HAP in various particles sizes, temperatures, initial
concentrations, pH and reaction times. In this study, the sorption of
Zinc ions from a Zn solution onto HAP particles with sizes of 1537.6
nm and 47.6 nm at three initial pH values of 4.50, 6.00 and 7.50 was
studied. The results showed that better performance was obtained
through a 47.6 nm particle size and higher pH values. The
experimental data were analyzed using Langmuir, Freundlich, and
Arrhenius equations for equilibrium, kinetic and thermodynamic
studies. The analysis showed a maximum adsorption capacity of NHAP
as being 1.562 mmol/g at a pH of 7.5 and small particle size.
Kinetically, the prepared N-HAP is a feasible sorbent that retains Zn
(II) ions through a favorable and spontaneous sorption process.
Abstract: Vinegar is a precious food additive and complement as well as effective preservative against food spoilage. Recently traditional vinegar production has been improved using various natural substrates and fruits such as grape, palm, cherry, coconut, date, sugarcane, rice and balsam. These neoclassical fermentations resulted in several vinegar types with different tastes, fragrances and nutritional values because of applying various acetic acid bacteria as starters. Acetic acid bacteria include genera Acetobacter, Gluconacetobacter and Gluconobacter according to latest edition of Bergy-s Manual of Systematic Bacteriology that classifies genera on the basis of their 16s RNA differences. Acetobacter spp as the main vinegar starters belong to family Acetobacteraceae that are gram negative obligate aerobes, chemoorganotrophic bacilli that are oxidase negative and oxidize ethanol to acetic acid. In this research we isolated and identified a native Acetobacter strain with high acetic acid productivity and tolerance against high ethanol concentrations from Iranian peach as a summer delicious fruit that is very susceptible to food spoilage and decay. We used selective and specific laboratorial culture media such as Standard GYC, Frateur and Carr medium. Also we used a new industrial culture medium and a miniature fermentor with a new aeration system innovated by Pars Yeema Biotechnologists Co., Isfahan Science and Technology Town (ISTT), Isfahan, Iran. The isolated strain was successfully cultivated in modified Carr media with 2.5% and 5% ethanol simultaneously in high temperatures, 34 - 40º C after 96 hours of incubation period. We showed that the increase of ethanol concentration resulted in rising of strain sensitivity to high temperature. In conclusion we isolated and characterized a new Acetobacter strain from Iranian peach that could be considered as a potential strain for production of a new vinegar type, peach vinegar, with a delicious taste and advantageous nutritional value in food biotechnology and industrial microbiology.
Abstract: The effects of commercial or bovine yeasts on the
performance and blood variables of broiler chickens intoxicated with
aflatoxin were investigated in broilers. Four hundred eighty broilers
(Arbor Acres; 3-wk-old) were randomly assigned to 4 groups. Each
group (120 broiler chickens) was further randomly divided into 6
replicates of 20 chickens. The treatments were control diet without
additives (treatment 1), 250 ppb AFB1 (treatment 2), commercial
yeast, Saccharomyces cerevisiae, (CY 2.5 x 107 CFU/g) + 250 ppb
AFB1 (treatment 3) and bovine yeast, Saccharomyces cerevisiae,
(BY 2.5 x 107 CFU/g + 250 ppb AFB1 (treatment 4). Complete
randomized design (CRD) was used in the experiment. Feed
consumption and body weight were recorded at every five-day
period. On day 42, carcass compositions were determined from 30
birds per treatment. While chicks were sacrificed, 3-4 ml blood
sample was taken and stored frozen at (-20°C) for serum chemical
analysis to determine effects of consumption of diets on blood
chemistry (total protein, albumin, glucose, urea, cholesterol and
triglycerides). There were no significant differences in ADFI among
the treatments(P>0.05). However, BWG, FCR and mortality were
highly significantly different (P
Abstract: In this paper, multi-processors job shop scheduling problems are solved by a heuristic algorithm based on the hybrid of priority dispatching rules according to an ant colony optimization algorithm. The objective function is to minimize the makespan, i.e. total completion time, in which a simultanous presence of various kinds of ferons is allowed. By using the suitable hybrid of priority dispatching rules, the process of finding the best solution will be improved. Ant colony optimization algorithm, not only promote the ability of this proposed algorithm, but also decreases the total working time because of decreasing in setup times and modifying the working production line. Thus, the similar work has the same production lines. Other advantage of this algorithm is that the similar machines (not the same) can be considered. So, these machines are able to process a job with different processing and setup times. According to this capability and from this algorithm evaluation point of view, a number of test problems are solved and the associated results are analyzed. The results show a significant decrease in throughput time. It also shows that, this algorithm is able to recognize the bottleneck machine and to schedule jobs in an efficient way.
Abstract: This is a genetic comparison study of Arabian Oryx
(Oryx leucoryx) population at two different locations (A &B) based
on nuclear microsatellite DNA markers. Arabian Oryx is listed as
vulnerable and endanger by the World Conservation Union (IUCN).
Thirty microsatellite markers from bovine family were applied to
investigate the genetic diversity of the Arabian Oryx and to set up a
molecular inventory. Among 30 microsatellite markers used, 13
markers were moderately polymorphic. Arabian Oryx at location A
has shown better gene diversity over location B. However, mean
number of alleles were less than location B. Data of within
population inbreeding coefficient indicates inbreeding at both
locations (A&B). Based on the analysis of polymorphic microsatellite
markers, the study revealed that Arabian Oryx need a genetically
designed breeding program.
Abstract: Aim of this work was to compare the efficacy of two
loading methods of proteins onto polymeric nanocarriers: adsorption
and encapsulation methods. Preliminary studies of protein loading
were done using Bovine Serum Albumin (BSA) as model protein.
Nanocarriers were prepared starting from polylactic co-glycolic acid
(PLGA) polymer; production methods used are two different variants
of emulsion evaporation method. Nanoparticles obtained were
analyzed in terms of dimensions by Dynamic Light Scattering and
Loading Efficiency of BSA by Bradford Assay. Loaded
nanoparticles were then submitted to in-vitro protein dissolution test
in order to study the effect of the delivery system on the release rate
of the protein.
Abstract: Grey mold on grape is caused by the fungus Botrytis
cinerea Pers. Trichodex WP, a new biofungicide, that contains fungal
spores of Trichoderma harzianum Rifai, was used for biological
control of Grey mold on grape. The efficacy of Trichodex WP has
been reported from many experiments. Experiments were carried out
in the locality – Banatski Karlovac, on grapevine species – talijanski
rizling. The trials were set according to instructions of methods
PP1/152(2) and PP1/17(3) , according to a fully randomized block
design. Phytotoxicity was estimated by PP methods 1/135(2), the
intensity of infection according to Towsend Heuberger , the
efficiency by Abbott, the analysis of variance with Duncan test and
PP/181(2). Application of Trichodex WP is limited to the first two
treatments. Other treatments are performed with the fungicides based
on a.i. procymidone, vinclozoline and iprodione.
Abstract: Cenozoic basalts found in Jiangsu province of eastern
China include tholeiites and alkali basalts. The present paper analyzed
the major, trace elements, rare earth elements of these Cenozoic
basalts and combined with Sr-Nd isotopic compositions proposed by
Chen et al. (1990)[1] in the literatures to discuss the petrogenesis of
these basalts and the geochemical characteristics of the source mantle.
Based on major, trace elements and fractional crystallization model
established by Brooks and Nielsen (1982)[2] we suggest that the
basaltic magma has experienced olivine + clinopyroxene fractionation
during its evolution. The chemical compositions of basaltic rocks from
Jiangsu province indicate that these basalts may belong to the same
magmatic system. Spidergrams reveal that Cenozoic basalts from
Jiangsu province have geochemical characteristics similar to those of
ocean island basalts(OIB). The slight positive Nb and Ti anomalies
found in basaltic rocks of this study suggest the presence of Ti-bearing
minerals in the mantle source and these Ti-bearing minerals had
contributed to basaltic magma during partial melting, indicating a
metasomatic event might have occurred before the partial melting.
Based on the Sr vs. Nd isotopic ratio plots, we suggest that Jiangsu
basalts may be derived from partial melting of mantle source which
may represent two-end members mixing of DMM and EM-I. Some
Jiangsu basaltic magma may be derived from partial melting of EM-I
heated by the upwelling asthenospheric mantle or asthenospheric
diapirism.
Abstract: Bovine viral diarrhea virus (BVDV) can cause lifelong
persistent infection. One reason for the phenomena is attributed to
BVDV infection to placenta tissue. However the mechanisms that
BVDV invades into placenta tissue remain unclear. To clarify the
molecular mechanisms, we investigated the possible means that
BVDV entered into bovine trophoblast cells (TPC). Yeast two-hybrid
system was used to identify proteins extracted from TPC, which
interact with BVDV envelope glycoprotein E2. A PGbkt7-E2 yeast
expression vector and TPC cDNA library were constructed. Through
two rounds of screening, three positive clones were identified.
Sequencing analysis indicated that all the three positive clones
encoded the same protein clathrin. Physical interaction between
clathrin and BVDV E2 protein was further confirmed by
coimmunoprecipitation experiments. This result suggested that the
clathrin might play a critical role in the process of BVDV entry into
placenta tissue and might be a novel antiviral target for preventing
BVDV infection.
Abstract: Results of Chilean wine classification based on the
information provided by an electronic nose are reported in this paper.
The classification scheme consists of two parts; in the first stage,
Principal Component Analysis is used as feature extraction method to
reduce the dimensionality of the original information. Then, Radial
Basis Functions Neural Networks is used as pattern recognition
technique to perform the classification. The objective of this study is
to classify different Cabernet Sauvignon, Merlot and Carménère wine
samples from different years, valleys and vineyards of Chile.
Abstract: This study was conducted Ismailoglu grape type (Vitis
vinifera L.) and its vine which was aged 15 was grown on its own
root in a vegetation period of 2013 in Nevşehir province in Turkey.
In this research, it was investigated whether the applications of
Control (C), 1/3 cluster tip reduction (1/3 CTR), shoot tip reduction
(STR), 1/3 CTR + STR, TKI-HUMAS (TKI-HM) (Soil) (S), TKIHM
(Foliar) (F), TKI-HM (S + F), 1/3 CTR + TKI-HM (S), 1/3 CTR
+ TKI-HM (F), 1/3 CTR + TKI-HM (S+F), STR + TKI-HM (S), STR
+ TKI-HM (F), STR + TKI-HM (S + F), 1/3 CTR + STR+TKI-HM
(S), 1/3 CTR + STR + TKI-HM (F), 1/3 CTR + STR + TKI-HM (S +
F) on yield and yield components of Ismailoglu grape type. The
results were obtained as the highest fresh grape yield (16.15 kg/vine)
with TKI-HM (S), as the highest cluster weight (652.39 g) with 1/3
CTR + STR, as the highest 100 berry weight (419.07 g) with 1/3
CTR + STR + TKI-HM (F), as the highest maturity index (44.06)
with 1/3 CTR, as the highest must yield (810.00 ml) with STR +
TKI-HM (F), as the highest intensity of L* color (42.04) with TKIHM
(S + F), as the highest intensity of a* color (2.60) with 1/3 CTR
+ TKI-HM (S), as the highest intensity of b* color (7.16) with 1/3
CTR + TKI-HM (S) applications. To increase the fresh grape yield of
Ismailoglu grape type can be recommended TKI-HM (S) application.
Abstract: In recent years, there has been an increasing interest
toward the use of bovine genotyped embryos for commercial embryo
transfer programs. Biopsy of a few cells in morulla stage is essential
for preimplantation genetic diagnosis (PGD). Low amount of DNA
have limited performing the several molecular analyses within PGD
analyses. Whole genome amplification (WGA) promises to eliminate
this problem. We evaluated the possibility and performance of an
improved primer extension preamplification (I-PEP) method with a
range of starting bovine genomic DNA from 1-8 cells into the WGA
reaction. We optimized a short and simple I-PEP (ssI-PEP) procedure
(~3h). This optimized WGA method was assessed by 6 loci specific
polymerase chain reactions (PCRs), included restriction fragments
length polymorphism (RFLP). Optimized WGA procedure possesses
enough sensitivity for molecular genetic analyses through the few
input cells. This is a new era for generating characterized bovine
embryos in preimplantation stage.
Abstract: For over a decade, the Pulse Coupled Neural Network
(PCNN) based algorithms have been successfully used in image
interpretation applications including image segmentation. There are
several versions of the PCNN based image segmentation methods,
and the segmentation accuracy of all of them is very sensitive to the
values of the network parameters. Most methods treat PCNN
parameters like linking coefficient and primary firing threshold as
global parameters, and determine them by trial-and-error. The
automatic determination of appropriate values for linking coefficient,
and primary firing threshold is a challenging problem and deserves
further research. This paper presents a method for obtaining global as
well as local values for the linking coefficient and the primary firing
threshold for neurons directly from the image statistics. Extensive
simulation results show that the proposed approach achieves
excellent segmentation accuracy comparable to the best accuracy
obtainable by trial-and-error for a variety of images.
Abstract: The deficit of power for electricity demand reaches
almost 30% for consumers in the last few years. This reflects with
continually increasing the price of electricity, and today the price for
small industry is almost 110Euro/MWh. The high price is additional
problem for the owners in the economy crisis which is reflected with
higher price of the goods.
The paper gives analyses of the energy needs for real agro
complex in Macedonia, private vinery with capacity of over 2 million
liters in a year and with self grapes and fruits fields. The existing
power supply is from grid with 10/04 kV transformer. The
geographical and meteorological condition of the vinery location
gives opportunity for including renewable as a power supply option
for the vinery complex.
After observation of the monthly energy needs for the vinery, the
base scenario is the existing power supply from the distribution grid.
The electricity bill in small industry has three factors: electricity in
high and low tariffs in kWh and the power engaged for the
technological process of production in kW. These three factors make
the total electricity bill and it is over 110 Euro/MWh which is the
price near competitive for renewable option. On the other side
investments in renewable (especially photovoltaic (PV)) has tendency
of decreasing with price of near 1,5 Euro/W. This means that
renewable with PV can be real option for power supply for small
industry capacities (under 500kW installed power).
Therefore, the other scenarios give the option with PV and the last
one includes wind option. The paper presents some scenarios for
power supply of the vinery as the followings:
• Base scenario of existing conventional power supply from the
grid
• Scenario with implementation of renewable of Photovoltaic
• Scenario with implementation of renewable of Photovoltaic and
Wind power
The total power installed in a vinery is near 570 kW, but the
maximum needs are around 250kW. At the end of the full paper some
of the results from scenarios will be presented. The paper also
includes the environmental impacts of the renewable scenarios, as
well as financial needs for investments and revenues from renewable.
Abstract: Enterprise Wide Information Systems (EWIS)
implementation involves the entire business and will require changes
throughout the firm. Because of the scope, complexity and
continuous nature of ERP, the project-based approach to managing
the implementation process resulted in failure rates of between 60%
and 80%. In recent years ERP systems have received much attention.
The organizational relevance and risk of ERP projects make it
important for organizations to focus on ways to make ERP
implementation successful. Once these systems are in place,
however, their performance depends on the identified macro
variables viz. 'Business Process', 'Decision Making' and 'Individual
/ Group working'. The questionnaire was designed and administered.
The responses from 92 organizations were compiled. The
relationship of these variables with EWIS performance is analyzed
using inferential statistical measurements. The study helps to
understand the performance of model presented. The study suggested
in keeping away from the calamities and thereby giving the
necessary competitive edge. Whenever some discrepancy is
identified during the process of performance appraisal care has to be
taken to draft necessary preventive measures. If all these measures
are taken care off then the EWIS performance will definitely deliver
the results.
Abstract: Cluster analysis is the name given to a diverse collection of techniques that can be used to classify objects (e.g. individuals, quadrats, species etc). While Kohonen's Self-Organizing Feature Map (SOFM) or Self-Organizing Map (SOM) networks have been successfully applied as a classification tool to various problem domains, including speech recognition, image data compression, image or character recognition, robot control and medical diagnosis, its potential as a robust substitute for clustering analysis remains relatively unresearched. SOM networks combine competitive learning with dimensionality reduction by smoothing the clusters with respect to an a priori grid and provide a powerful tool for data visualization. In this paper, SOM is used for creating a toroidal mapping of two-dimensional lattice to perform cluster analysis on results of a chemical analysis of wines produced in the same region in Italy but derived from three different cultivators, referred to as the “wine recognition data" located in the University of California-Irvine database. The results are encouraging and it is believed that SOM would make an appealing and powerful decision-support system tool for clustering tasks and for data visualization.
Abstract: Polarization modulation infrared reflection absorption
spectroscopy (PM-IRRAS) in combination with electrochemistry,
was employed to study the influence of surface charge (potential) on
the kinetics of bovine serum albumin (BSA) adsorption on a
biomedical-grade 316LVM stainless steel surface is discussed. The
BSA adsorption kinetics was found to greatly depend on the surface
potential. With an increase in surface potential towards more
negative values, both the BSA initial adsorption rate and the
equilibrium (saturated) surface concentration also increased. Both
effects were explained on the basis of replacement of well-ordered
water molecules at the 316LVM / solution interface, i.e. by the
increase in entropy of the system.
Abstract: The objective of the present study was to evaluate the
potential of hollow microneedles for enhancing the transdermal
delivery of Bovine Serum Albumin (MW~66,000 Da)-Fluorescein
Isothiocyanate (BSA-FITC) conjugate, a hydrophilic large molecular
compound. Moreover, the effect of different formulations was
evaluated. The series of binary mixtures composed of propylene
glycol (PG) and pH 7.4 phosphate buffer solution (PBS) was
prepared and used as a medium for BSA-FITC. The results showed
that there was no permeation of BSA-FITC solution across the
neonatal porcine skin without using hollow microneedles, whereas
the cumulative amount of BSA-FITC released at 8 h through the
neonatal porcine skin was about 60-70% when using hollow
microneedles. Furthermore, the results demonstrated that the higher
volume of PG in binary mixtures injected, the lower cumulative
amount of BSA-FITC released and release rate of BSA-FITC from
skin. These release profiles of BSA-FITC in binary mixtures were
expressed by Fick-s law of diffusion. These results suggest the
utilization of hollow microneedle to enhance transdermal delivery of
protein and provide useful information for designing an effective
hollow microneedle system.
Abstract: Data Mining aims at discovering knowledge out of
data and presenting it in a form that is easily comprehensible to
humans. One of the useful applications in Egypt is the Cancer
management, especially the management of Acute Lymphoblastic
Leukemia or ALL, which is the most common type of cancer in
children.
This paper discusses the process of designing a prototype that can
help in the management of childhood ALL, which has a great
significance in the health care field. Besides, it has a social impact
on decreasing the rate of infection in children in Egypt. It also
provides valubale information about the distribution and
segmentation of ALL in Egypt, which may be linked to the possible
risk factors.
Undirected Knowledge Discovery is used since, in the case of this
research project, there is no target field as the data provided is
mainly subjective. This is done in order to quantify the subjective
variables. Therefore, the computer will be asked to identify
significant patterns in the provided medical data about ALL. This
may be achieved through collecting the data necessary for the
system, determimng the data mining technique to be used for the
system, and choosing the most suitable implementation tool for the
domain.
The research makes use of a data mining tool, Clementine, so as to
apply Decision Trees technique. We feed it with data extracted from
real-life cases taken from specialized Cancer Institutes. Relevant
medical cases details such as patient medical history and diagnosis
are analyzed, classified, and clustered in order to improve the disease
management.
Abstract: According to FDA (Food and Drug Administration of the United States), vinegar is definedas a sour liquid containing at least 4 grams acetic acid in 100 cubic centimeter (4% solution of acetic acid) of solution that is produced from sugary materials by alcoholic fermentation. In the base of microbial starters, vinegars could be contained of more than 50 types of volatile and aromatic substances that responsible for their sweet taste and smelling. Recently the vinegar industry has a great proportion in agriculture, food and microbial biotechnology. The acetic acid bacteria are from the family Acetobacteraceae. Regarding to the latest version of Bergy-s Mannual of Systematic Bacteriology that has categorized bacteria in the base of their 16s RNA differences, the most important acetic acid genera are included Acetobacter (genus I), Gluconacetobacter (genus VIII) and Gluconobacter (genus IX). The genus Acetobacter that is primarily used in vinegar manufacturing plants is a gram negative, obligate aerobe coccus or rod shaped bacterium with the size 0.6 - 0.8 X 1.0 - 4.0 μm, nonmotile or motile with peritrichous flagella and catalase positive – oxidase negative biochemically. Some strains are overoxidizer that could convert acetic acid to carbon dioxide and water.In this research one Acetobacter native strain with high acetic acid productivity was isolated from Iranian white – red cherry. We used two specific culture media include Carr medium [yeast extract, 3%; ethanol, 2% (v/v); bromocresol green, 0.002%; agar, 2% and distilled water, 1000 ml], Frateur medium [yeast extract, 10 g/l; CaCO3, 20 g/l; ethanol, 20 g/l; agar, 20 g/l and distilled water, 1000 ml] and an industrial culture medium. In addition to high acetic acid production and high growth rate, this strain had a good tolerance against ethanol concentration that was examined using modified Carr media with 5%, 7% and 9% ethanol concentrations. While the industrial strains of acetic acid bacteria grow in the thermal range of 28 – 30 °C, this strain was adapted for growth in 34 – 36 °C after 96 hours incubation period. These dramatic characteristics suggest a potential biotechnological strain in production of cherry vinegar with a sweet smell and different nutritional properties in comparison to recent vinegar types. The lack of growth after 24, 48 and 72 hours incubation at 34 – 36 °C and the growth after 96 hours indicates a good and fast thermal flexibility of this strain as a significant characteristic of biotechnological and industrial strains.