Abstract: The Egyptian Bacillus thuringiensis isolate (M5) produce crystal proteins that is toxic against insects was irradiated with UV light to induce mutants. Upon testing 10 of the resulting mutants for their toxicity against cotton leafworm larvae, the three mutants 62, 64 and 85 proved to be the most toxic ones. Upon testing these mutants along with their parental isolate by SDS-PAGE analysis of spores-crystals proteins as well as vegetative cells proteins, new induced bands appeared in the three mutants by UV radiation and also they showed disappearance of some other bands as compared with the wild type isolate. Multiplex PCR technique, with five sets of specific primers, was used to detect the three types of cryI genes cryIAa, cryIAb and cryIAc. Results showed that these three genes exist, as distinctive bands, in the wild type isolate (M5) as well as in mutants 62 and 85, while the mutant 64 had two distinctive bands of cryIAb and cryIAc genes, and a faint band of cryI Aa gene. Finally, these results revealed that mutant 62 is considered as the promising mutant since it is UV resistant, highly toxic against Spodoptera littoralis and active against a wide range of Lepidopteran insects.
Abstract: Our results showed that for the growth of qualitative
seedling and vegetative raw material of ðó. marschallianus Willd. and
T. serphyllum L. it is more profitable to use the in vitro and
hydroponics combined method. In in vitro culture it is possible to do
micro-propagation whole year with 98-99% rhizogenesis. 30000
micro-plants were obtained from one explant during 9 months.
Hydroponic conditions provide the necessary microclimate for
microplants where the survival rate without acclimatization was
93.3%. The essential oil content in hydroponic dry herb of both
species in vegetative and blossom phase was 1.3% whereas in wild
plants it was 1.2%, the content of extractive substances and vitamin
C also exceeded wild plants. Our biochemical and radiochemical
investigations indicated that the medicinal raw materials obtained
from hydroponic and wild plants of Thymus species correspond to
the demands of SPh XI, and the content of artificial radionuclides
does not exceed the MACL.
Abstract: The studies concerned an effect of six variants of ion
exchange substrate (nutrient carriers with a different potential impact
on pH of soil solution) on vegetation of orchard grass during two
different periods (42 and 84 days). In the pot experiment plants were
grown on sand (model of degraded soil) and six mixtures of sand and
2% (v/v) additions of particular variants of ion exchange substrate
(with pH ranged from 5.5 to 8.0). The study results showed that the
addition of the substrate at pH=6.5 caused the highest increase in
plant yield after shorter vegetation period whereas the addition of the
substrate at pH=5.5 increased dry stem and root biomass of orchard
grass after longer vegetation period. Thus, the ion exchange substrate
at pH=6.5 can be recommended for restoration of exhausted soils
when shorter vegetation period is planned; the ion exchange substrate
at pH=5.5 can be used for the same purpose when longer periods of
vegetative growth are considered.
Abstract: New biostimulator from wheat seeds which by its
chemical composition relates to fusicoccin is presented in this article.
New biostimulator could be used as powerful hormonal substance
that has ability to increase productivity and salt tolerance of
agricultural plants. Also on the basis of biostimulator we have
developed vegetative method for fast reproduction of perennial plants
as desert plant - Tamarix gracilis.
Abstract: To evaluate genetic variation of wheat (Triticum aestivum) affected by heat and drought stress on eight Australian wheat genotypes that are parents of Doubled Haploid (HD) mapping populations at the vegetative stage, the water stress experiment was conducted at 65% field capacity in growth room. Heat stress experiment was conducted in the research field under irrigation over summer. Result show that water stress decreased dry shoot weight and RWC but increased osmolarity and means of Fv/Fm values in all varieties except for Krichauff. Krichauff and Kukri had the maximum RWC under drought stress. Trident variety was shown maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield and Fv/Fm 0.815 under water stress condition. However, the recovery of quantum yield was apparent between 4 to 7 days after stress in all varieties. Nevertheless, increase in water stress after that lead to strong decrease in quantum yield. There was a genetic variation for leaf pigments content among varieties under heat stress. Heat stress decreased significantly the total chlorophyll content that measured by SPAD. Krichauff had maximum value of Anthocyanin content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b (0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content belonged to Kukri. The quantum yield of all varieties decreased significantly, when the weather temperature increased from 28 ÔùªC to 36 ÔùªC during the 6 days. However, the recovery of quantum yield was apparent after 8th day in all varieties. The maximum decrease and recovery in quantum yield was observed in Krichauff. Drought and heat tolerant and moderately tolerant wheat genotypes were included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut and Excalibur were clustered into most sensitive and moderately sensitive genotypes. Finally, the results show that there was a significantly genetic variation among the eight varieties that were studied under heat and water stress.
Abstract: Heavy metal pollution is an environmental concern.
Phytoremediation is a low-cost, environmental-friendly approach to
solve this problem. Mustard has the potential in reducing heavy metal
contents in soils. Among mustard (Brassica juncea (L.) Czern &
Coss) genotypes in Sri Lanka, accessions 7788, 8831 and 5088 give
significantly a high yield. Therefore, present study was conducted to
quantify the phytoextractive potential among these local mustard
accessions and to assess the interaction of heavy metals, Pb, Co, Mn
on phytoextraction. A pot experiment was designed with acid washed
sand (quartz) and a series of heavy metal solutions of 0, 25, 50, 75
and 100 μg/g. Experiment was carried out with factorial
experimental design. Mustard accessions were tolerant to heavy
metals and could be successfully used in removal of Pb, Co and Mn
and they are capable of accumulating significant quantities of heavy
metals in vegetative and reproductive organs. The order of the
accumulative potential of Pb, Co and Mn in mustard accessions is,
root > shoot >seed.
Abstract: Photoselective plastic films with thermic properties
are now available so that greenhouses clad with such plastics exhibit
a higher degree of “Greenhouse Effect” with a consequent increase in
night time temperature. In this study, we investigate the potential
benefits of a range of thermic plastic films used as greenhouse cover
materials on the vegetative and reproductive growth and development
of Iceberg lettuce (Lactuca sativa L). Transplants were grown under
thermic films and destructively harvested 4, 5, and 6 weeks after
transplanting. Thermic films can increase night temperatures up to 2
⁰C reducing the wide fluctuation in greenhouse temperature during
winter compared to the standard commercial film and consequently
increased the yield (leaf number, fresh weight, and dry weight) of
lettuce plants. Lettuce plants grown under Clear film respond to cold
stress by the accumulation of secondary products (phenolics, and
flavonoids).
Abstract: Article devoted to the development of technologies
for medicine and agroecology by using plant organelle – spherosome.
Technological method of purification and isolation of this organelle
by using novel nanostructured carbon sorbent – “nanocarbosorb"
ARK type are presented. Also the methods of preparation of
nanocontainers based on using of spherosome with loaded isosorbide
dinitrate, piroxicam or diclofenak are exhibited. We found that the
spherosome could be applied for ecological aims as bioregulator and
also as biosensor for determination of ammonia ions in water
reservoirs at concentration range 1mM to 100mM.
Abstract: The trial in the city, located 170 kilometers from the
Iranian city of Ahvaz was Omidiyeh. The main factor in this project
includes 4 levels in control (without hormones), use of hormones in
the seed, vegetative and flowering stage respectively. And sub-plots
included 3 varieties of vetch in three levels, with local names, was the
jewel in the study of light and Auxin in the vegetative and
reproductive different times in different varieties of vetch was
investigated. This test has been taken in the plots in a randomized
complete block with four replications. In order to study the effects of
the hormone Auxin in the growth stages (seed, vegetative and
flowering) to control (no hormone Auxin) on three local varieties of
vetch, the essence of light and plant height, number of pods per plant,
seed number The pods, seeds per plant, grain weight, grain yield,
plant dry weight and protein content were measured. Among the
vetch varieties for plant height, number of pods per plant, a seed per
plant, grain weight, grain yield, and plant dry weight and protein
levels of 1 percent of plant and seed number per pod per plant at 5%
level of There was no significant difference. Interactions for grain
yield per plant, grain yield and protein levels of 1 percent and the
number of seeds per pod and seed weight are significant differences
in levels 5 and plant height and plant dry weight of the interaction
were INFLUENCE There was no significant difference in them.
Abstract: To evaluate genetic variation of wheat (Triticum
aestivum) affected by heat and drought stress on eight Australian
wheat genotypes that are parents of Doubled Haploid (HD) mapping
populations at the vegetative stage, the water stress experiment was
conducted at 65% field capacity in growth room. Heat stress
experiment was conducted in the research field under irrigation over
summer. Result show that water stress decreased dry shoot weight
and RWC but increased osmolarity and means of Fv/Fm values in all
varieties except for Krichauff. Krichauff and Kukri had the
maximum RWC under drought stress. Trident variety was shown
maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield
and Fv/Fm 0.815 under water stress condition. However, the
recovery of quantum yield was apparent between 4 to 7 days after
stress in all varieties. Nevertheless, increase in water stress after that
lead to strong decrease in quantum yield. There was a genetic
variation for leaf pigments content among varieties under heat stress.
Heat stress decreased significantly the total chlorophyll content that
measured by SPAD. Krichauff had maximum value of Anthocyanin
content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and
chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b
(0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content
belonged to Kukri. The quantum yield of all varieties decreased
significantly, when the weather temperature increased from 28 ÔùªC to
36 ÔùªC during the 6 days. However, the recovery of quantum yield
was apparent after 8th day in all varieties. The maximum decrease
and recovery in quantum yield was observed in Krichauff. Drought
and heat tolerant and moderately tolerant wheat genotypes were
included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut
and Excalibur were clustered into most sensitive and moderately
sensitive genotypes. Finally, the results show that there was a
significantly genetic variation among the eight varieties that were
studied under heat and water stress.
Abstract: An experiment was implemented in a filed in the south of Morocco to evaluate the effects of domestic treated wastewater use for irrigation of amaranth crop under semi-arid conditions. Three varieties (A0020, A0057 & A211) were tested and irrigated using domestic treated wastewater EC1 (0,92 dS/m) as control, EC3 (3dS/m) and EC6 (6dS/m) obtained by adding sea water. In term of growth, an increase of the EC level of applied irrigation water reduced significantly the plant-s height, leaf area, fresh and dry weight measured at vegetative, flowering and maturity stage for all varieties. Even with the application of the EC6, yields were relatively higher in comparison with the once obtained in normal cultivation conditions. A significant accumulation of nitrate, chloride and sodium in soil layers during the crop cycle was noted. The use of treated waste water for its irrigation is proved to be possible. The variety A211 had showed to be less sensitive to salinity stress and it could be more promising its introduction to study area.
Abstract: Yield and Crop Water Productivity are crucial issues
in sustainable agriculture, especially in high-demand resource crops such as sweet corn. This study was conducted to investigate
agronomic responses such as plant growth, yield and soil parameters (EC and Nitrate accumulation) to several deficit irrigation treatments
(100, 75, 50, 25 and 0% of ETm) applied during vegetative growth
stage, rainfed treatment was also tested.
The finding of this research indicates that under deficit irrigation
during vegetative growth stage applying 75% of ETm lead to increasing of 19.4% in terms of fresh ear yield, 9.4% in terms of dry grain yield, 10.5% in terms of number of ears per plant, 11.5% for
the 1000 grains weight and 19% in terms of crop water productivity compared with fully irrigated treatment. While those parameters in
addition to root, shoot and plant height has been affected by deficit
irrigation during vegetative growth stage when increasing water stress degree more than 50% of ETm.
Abstract: Two indica varieties, IR36 and ‘Suweon 258’ (“S”)
are middle-heading in southern Japan. 36U, also middle-heading, is
an isogenic line of IR36 carrying Ur1 (Undulate rachis-1) gene.
However, late-heading plants segregated in the F2 population from
the F1 of S × 36U, and so did in the following generations. The
concerning lateness gene is designated as Ex. From the F8 generation,
isogenic-line pair of early-heading and late-heading lines, denoted by
“E” (ex/ex) and “L” (Ex/Ex), were developed. Genetic analyses of
heading time were conducted, using F1s and F2s among L, E, S and
36U. The following inferences were drawn from the experimental
results: 1) L, and both of E and 36U harbor Ex and ex, respectively;
2) Besides Ex, S harbors an inhibitor gene to it, i.e. I-Ex which is a
novel finding of the present study. 3) Ex is a dominant allele at the
E1 locus.