Abstract: The dynamic speckle or biospeckle is an interference
phenomenon generated at the reflection of a coherent light by an
active surface or even by a particulate or living body surface. The
above mentioned phenomenon gave scientific support to a method
named biospeckle which has been employed to study seed viability,
biological activity, tissue senescence, tissue water content, fruit
bruising, etc. Since the above mentioned method is not invasive and
yields numerical values, it can be considered for possible automation
associated to several processes, including selection and sorting.
Based on these preliminary considerations, this research work
proposed to study the interaction of a laser beam with vegetative
samples by measuring the incident light intensity and the transmitted
light beam intensity at several vegetative slabs of varying thickness.
Tests were carried on fifteen slices of apple tissue divided into three
thickness groups, i.e., 4 mm, 5 mm, 18 mm and 22 mm. A diode laser
beam of 10mW and 632 nm wavelength and a Samsung digital
camera were employed to carry the tests. Outgoing images were
analyzed by comparing the gray gradient of a fixed image column of
each image to obtain a laser penetration scale into the tissue,
according to the slice thickness.