Abstract: The image segmentation method described in this
paper has been developed as a pre-processing stage to be used in
methodologies and tools for video/image indexing and retrieval by
content. This method solves the problem of whole objects extraction
from background and it produces images of single complete objects
from videos or photos. The extracted images are used for calculating
the object visual features necessary for both indexing and retrieval
processes.
The segmentation algorithm is based on the cooperation among an
optical flow evaluation method, edge detection and region growing
procedures. The optical flow estimator belongs to the class of
differential methods. It permits to detect motions ranging from a
fraction of a pixel to a few pixels per frame, achieving good results in
presence of noise without the need of a filtering pre-processing stage
and includes a specialised model for moving object detection.
The first task of the presented method exploits the cues from
motion analysis for moving areas detection. Objects and background
are then refined using respectively edge detection and seeded region
growing procedures. All the tasks are iteratively performed until
objects and background are completely resolved.
The method has been applied to a variety of indoor and outdoor
scenes where objects of different type and shape are represented on
variously textured background.
Abstract: The counting process of cell colonies is always a long
and laborious process that is dependent on the judgment and ability
of the operator. The judgment of the operator in counting can vary in
relation to fatigue. Moreover, since this activity is time consuming it
can limit the usable number of dishes for each experiment. For these
purposes, it is necessary that an automatic system of cell colony
counting is used. This article introduces a new automatic system of
counting based on the elaboration of the digital images of cellular
colonies grown on petri dishes. This system is mainly based on the
algorithms of region-growing for the recognition of the regions of
interest (ROI) in the image and a Sanger neural net for the
characterization of such regions. The better final classification is
supplied from a Feed-Forward Neural Net (FF-NN) and confronted
with the K-Nearest Neighbour (K-NN) and a Linear Discriminative
Function (LDF). The preliminary results are shown.