Abstract: Preparation of nanoparticles of cerium oxide and
adsorption of bovine serum albumin on them were studied. Particle
size distribution and influence of pH on zeta potential of prepared
CeO2 were determined. Average size of prepared cerium oxide
nanoparticles was 9 nm. The simultaneous measurements of the
bovine serum albumin adsorption and zeta potential determination of
the (adsorption) suspensions were carried out. The adsorption
isotherms were found to be of typical Langmuir type; values of the
bovine serum albumin adsorption capacities were calculated.
Increasing of pH led to decrease of zeta potential and decrease of
adsorption capacity of cerium oxide nanoparticles. The maximum
adsorption capacity was found for strongly acid suspension (am =
118 mg/g). The samples of nanoceria with positive zeta potential
adsorbed more bovine serum albumin on the other hand, the samples
with negative zeta potential showed little or no protein adsorption.
Surface charge or better say zeta potential of CeO2 nanoparticles
plays the key role in adsorption of proteins on such type of materials.
Abstract: A major challenge in biomaterials research is the
regulation of protein adsorption which is a key factor for controlling
the subsequent cell adhesion at implant surfaces. The aim of the
present study was to control the adsorption of fibronectin (FN) and
the attachment of MG-63 osteoblasts with an electronic
nanostructure. Shallow doping line lattices with a period of 260 nm
were produced for this purpose by implantation of phosphorous in
silicon wafers. Protein coverage was determined after incubating the
substrate with FN by means of an immunostaining procedure and the
measurement of the fluorescence intensity with a TECAN analyzer.
We observed an increased amount of adsorbed FN on the
nanostructure compared to control substrates. MG-63 osteoblasts
were cultivated for 24h on FN-incubated substrates and their
morphology was assessed by SEM. Preferred orientation and
elongation of the cells in direction of the doping lattice lines was
observed on FN-coated nanostructures.