Alignment of MG-63 Osteoblasts on Fibronectin-Coated Phosphorous Doping Lattices in Silicon
A major challenge in biomaterials research is the
regulation of protein adsorption which is a key factor for controlling
the subsequent cell adhesion at implant surfaces. The aim of the
present study was to control the adsorption of fibronectin (FN) and
the attachment of MG-63 osteoblasts with an electronic
nanostructure. Shallow doping line lattices with a period of 260 nm
were produced for this purpose by implantation of phosphorous in
silicon wafers. Protein coverage was determined after incubating the
substrate with FN by means of an immunostaining procedure and the
measurement of the fluorescence intensity with a TECAN analyzer.
We observed an increased amount of adsorbed FN on the
nanostructure compared to control substrates. MG-63 osteoblasts
were cultivated for 24h on FN-incubated substrates and their
morphology was assessed by SEM. Preferred orientation and
elongation of the cells in direction of the doping lattice lines was
observed on FN-coated nanostructures.
[1] C.J. Bettinger, R. Langer, J.T. Borenstein, Engineering Substrate
Topography at the Micro- and Nanoscale to Control Cell Function,
Angew. Chem. Int. Ed. 48 (2009) 5406-5415.
[2] C. Galli, M.C. Coen, R. Hauert, V.L. Katanaev, M.P. Wymann, P.
Gröning, L. Schlapbach, Protein adsorption on topographically
nanostructured titanium, Surface Science 474 (2001) L180-L184.
[3] P. Roach, D. Farrar, C.C. Perry, Surface Tailoring for Controlled Protein
Adsorption: Effect of Topography at the Nanometer Scale and
Chemistry, J. Am. Chem. Soc. 128 (2006) 3939-3945.
[4] P. Elter, R. Lange, U. Beck, Atomic force microscopy studies of the
influence of convex and concave nanostructures on the adsorption of
fibronectin, Colloids and surfaces 89 (2012) 139-146.
[5] G.B. Sigal, M. Mrksich, G.M. Whitesides, Effect of Surface Wettability
on the Adsorption of Proteins and Detergents, J. Am. Chem. Soc. 120
(1998) 3464-3473.
[6] Y. Arima, H. Iwata, Effect of wettability and surface functional groups
on protein adsorption and cell adhesion using well-defined mixed selfassembled
monolayers, Biomaterials 28 (2007) 3074-3082.
[7] M. Birkholz, P. Zaumseil, J. Bauer, D. Bolze, G. Weidner, Small-angle
reciprocal space mapping of surface relief gratings, Materials science &
engineering 27 (2007) 1154-1157.
[8] D. Knoll, K. Ehwald, B. Heinemann, A. Fox, K. Blum, H. Rucker, F.
Furnhammer, B. Senapati, R. Barth, U. Haak, W. Hoppner, J. Drews, R.
Kurps, S. Marschmeyer, H. Richter, T. Grabolla, B. Kuck, O. Fursenko,
P. Schley, R. Scholz, B. Tillack, Y. Yamamoto, K. Kopke, H. Wulf, D.
Wolansky, W. Winkler, A flexible, low-cost, high performance SiGe:C
BiCMOS process with a one-mask HBT module, pp. 783-786.
[9] D. Knoll, B. Heinemann, R. Barth, K. Blum, J. Borngraber, J. Drews,
K.-E. Ehwald, G. Fischer, A. Fox, T. Grabolla, U. Haak, W. Hoppner, F.
Korndorfer, B. Kuck, S. Marschmeyer, H. Richter, H. Rucker, P. Schley,
D. Schmidt, R. Scholz, B. Senapati, B. Tillack, W. Winkler, D.
Wolansky, C. Wolf, H.-E. Wulf, Y. Yamamoto, P. Zaumseil, A modular,
low-cost SiGe:C BiCMOS process featuring high-f/sub T/ and high
BV/sub CEO/ transistors, pp. 241-244.
[10] F. Grinnell, M.K. Feld, Fibronectin adsorption on hydrophilic and
hydrophobic surfaces detected by antibody binding and analyzed during
cell adhesion in serum-containing medium, J. Biol. Chem.257 (1982)
4888-4893.
[1] C.J. Bettinger, R. Langer, J.T. Borenstein, Engineering Substrate
Topography at the Micro- and Nanoscale to Control Cell Function,
Angew. Chem. Int. Ed. 48 (2009) 5406-5415.
[2] C. Galli, M.C. Coen, R. Hauert, V.L. Katanaev, M.P. Wymann, P.
Gröning, L. Schlapbach, Protein adsorption on topographically
nanostructured titanium, Surface Science 474 (2001) L180-L184.
[3] P. Roach, D. Farrar, C.C. Perry, Surface Tailoring for Controlled Protein
Adsorption: Effect of Topography at the Nanometer Scale and
Chemistry, J. Am. Chem. Soc. 128 (2006) 3939-3945.
[4] P. Elter, R. Lange, U. Beck, Atomic force microscopy studies of the
influence of convex and concave nanostructures on the adsorption of
fibronectin, Colloids and surfaces 89 (2012) 139-146.
[5] G.B. Sigal, M. Mrksich, G.M. Whitesides, Effect of Surface Wettability
on the Adsorption of Proteins and Detergents, J. Am. Chem. Soc. 120
(1998) 3464-3473.
[6] Y. Arima, H. Iwata, Effect of wettability and surface functional groups
on protein adsorption and cell adhesion using well-defined mixed selfassembled
monolayers, Biomaterials 28 (2007) 3074-3082.
[7] M. Birkholz, P. Zaumseil, J. Bauer, D. Bolze, G. Weidner, Small-angle
reciprocal space mapping of surface relief gratings, Materials science &
engineering 27 (2007) 1154-1157.
[8] D. Knoll, K. Ehwald, B. Heinemann, A. Fox, K. Blum, H. Rucker, F.
Furnhammer, B. Senapati, R. Barth, U. Haak, W. Hoppner, J. Drews, R.
Kurps, S. Marschmeyer, H. Richter, T. Grabolla, B. Kuck, O. Fursenko,
P. Schley, R. Scholz, B. Tillack, Y. Yamamoto, K. Kopke, H. Wulf, D.
Wolansky, W. Winkler, A flexible, low-cost, high performance SiGe:C
BiCMOS process with a one-mask HBT module, pp. 783-786.
[9] D. Knoll, B. Heinemann, R. Barth, K. Blum, J. Borngraber, J. Drews,
K.-E. Ehwald, G. Fischer, A. Fox, T. Grabolla, U. Haak, W. Hoppner, F.
Korndorfer, B. Kuck, S. Marschmeyer, H. Richter, H. Rucker, P. Schley,
D. Schmidt, R. Scholz, B. Senapati, B. Tillack, W. Winkler, D.
Wolansky, C. Wolf, H.-E. Wulf, Y. Yamamoto, P. Zaumseil, A modular,
low-cost SiGe:C BiCMOS process featuring high-f/sub T/ and high
BV/sub CEO/ transistors, pp. 241-244.
[10] F. Grinnell, M.K. Feld, Fibronectin adsorption on hydrophilic and
hydrophobic surfaces detected by antibody binding and analyzed during
cell adhesion in serum-containing medium, J. Biol. Chem.257 (1982)
4888-4893.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:63359", author = "Andreas Körtge and Susanne Stählke and Regina Lange and Mario Birkholz and Mirko Fraschke and Katrin Schulz and Barbara Nebe and Patrick Elter", title = "Alignment of MG-63 Osteoblasts on Fibronectin-Coated Phosphorous Doping Lattices in Silicon", abstract = "A major challenge in biomaterials research is the
regulation of protein adsorption which is a key factor for controlling
the subsequent cell adhesion at implant surfaces. The aim of the
present study was to control the adsorption of fibronectin (FN) and
the attachment of MG-63 osteoblasts with an electronic
nanostructure. Shallow doping line lattices with a period of 260 nm
were produced for this purpose by implantation of phosphorous in
silicon wafers. Protein coverage was determined after incubating the
substrate with FN by means of an immunostaining procedure and the
measurement of the fluorescence intensity with a TECAN analyzer.
We observed an increased amount of adsorbed FN on the
nanostructure compared to control substrates. MG-63 osteoblasts
were cultivated for 24h on FN-incubated substrates and their
morphology was assessed by SEM. Preferred orientation and
elongation of the cells in direction of the doping lattice lines was
observed on FN-coated nanostructures.", keywords = "Cell adhesion, electronic nanostructures, doping lattice, fibronectin, MG-63 osteoblasts, protein adsorption.", volume = "7", number = "1", pages = "71-4", }