Abstract: An optimum technic has been developed for extracting volatile organic compounds which contribute to the aroma of lime fruit (Citrus aurantifolia). The volatile organic compounds of healthy and infested lime fruit with California red scale Aonidiella aurantii were characterized using headspace solid phase microextraction (HS-SPME) combined with gas chromatography (GC) coupled flame ionization detection (FID) and gas chromatography with mass spectrometry (GC-MS) as a very simple, efficient and nondestructive extraction method. A three-phase 50/30 μm PDV/DVB/CAR fibre was used for the extraction process. The optimal sealing and fibre exposure time for volatiles reaching equilibrium from whole lime fruit in the headspace of the chamber was 16 and 4 hours respectively. 5 min was selected as desorption time of the three-phase fibre. Herbivorous activity induces indirect plant defenses, as the emission of herbivorous-induced plant volatiles (HIPVs), which could be used by natural enemies for host location. GC-MS analysis showed qualitative differences among volatiles emitted by infested and healthy lime fruit. The GC-MS analysis allowed the initial identification of 18 compounds, with similarities higher than 85%, in accordance with the NIST mass spectral library. One of these were increased by A. aurantii infestation, D-limonene, and three were decreased, Undecane, α-Farnesene and 7-epi-α-selinene. From an applied point of view, the application of the above-mentioned VOCs may help boost the efficiency of biocontrol programs and natural enemies’ production techniques.
Abstract: Currently, biological control programs in greenhouse
crops involve the use, at the same time, several natural enemies
during the crop cycle. Also, large number of plant species grown in
greenhouses, among them, the used cultivars are also wide. However,
the cultivar effects on entomophagous species efficacy (predators and
parasitoids) have been scarcely studied. A new method had been
developed, using the factitious prey or host Ephestia kuehniella. It
allow us to evaluate, under greenhouse or controlled conditions
(semi-field), the cultivar effects on the entomophagous species
effectiveness. The work was carried out in greenhouse tomato crop. It
has been found the biological and ecological activities of predatory
species (Nesidiocoris tenuis) and egg-parasitoid (Trichogramma
achaeae) can be well represented with the use of the factitious prey
or host; being better in the former than the latter. The data found in
the trial are shown and discussed. The developed method could be
applied to evaluate new plant materials before making available to
farmers as commercial varieties, at low costs and easy use.
Abstract: Studies regarding the determination of population
trend of Lipaphis erysimi (kalt.) and its associated natural enemies in
different Brassica lines along with the effect of gamma radiation on
their population were conducted at Agricultural Research Farm,
Malakandher, Khyber Pakhtunkhwa Agricultural University
Peshawar during spring 2006. Three different Brassica lines F6B3,
F6B6 and F6B7 were used, which were replicated four times in
Randomized Complete Block Design. The data revealed that aphid
infestation invariably stated in all three varieties during last week of
February 2006 (1st observation). The peak population of 4.39 aphids
leaf-1 was s recorded during 2nd week of March and lowest population
of 1.02 aphids leaf-1 was recorded during 5th week of March. The
species of lady bird beetle (Coccinella septempunctata) and Syrphid
fly (Syrphus balteatus) first appeared on 24th February with a mean
number of 0.40 lady bird beetle leaf-1 and 0.87 Syrphid fly leaf-1,
respectively. At the time when aphid population started to increase
the peak population of C. septempunctata (0.70 lady bird beetle leaf-
1) and S. balteatus (1.04 syrphid fly leaf-1) was recorded on the 2nd
week of March. Chrysoperla carnea appeared in the 1st week of
March and their peak population was recorded during the 3rd week of
March with mean population of 1.46 C. carnea leaf-1. Among all the
Brassica lines, F6B7 showed comparatively more resistance as
compared to F6B3 F6B6. F6B3 showed least resistance against L.
erysimi, which was found to be the most susceptible cultivar. F6B7
was also found superior in terms of natural enemies. Maximum
number of all natural enemies was recorded on this variety followed
by F6B6. Lowest number of natural enemies was recorded in F6B3.
No significant effect was recorded for the effect of gamma radiation
on the population of aphids, natural enemies and on the varieties.