Abstract: The increasing high price of natural gas and oil with attendant increase in energy demand on world markets in recent years has stimulated interest in recovering residual oil saturation across the globe. In order to meet the energy security, efforts have been made in developing new technologies of enhancing the recovery of oil and gas, utilizing techniques like CO2 flooding, water injection, hydraulic fracturing, surfactant flooding etc. Surfactant flooding however optimizes production but poses risk to the environment due to their toxic nature. Amongst proven records that have utilized other type of bacterial in producing biosurfactants for enhancing oil recovery, this research uses a technique to combine biosurfactants that will achieve a scale of EOR through lowering interfacial tension/contact angle. In this study, three biosurfactants were produced from three Bacillus species from freeze dried cultures using sucrose 3 % (w/v) as their carbon source. Two of these produced biosurfactants were screened with the TEMCO Pendant Drop Image Analysis for reduction in IFT and contact angle. Interfacial tension was greatly reduced from 56.95 mN.m-1 to 1.41 mN.m-1 when biosurfactants in cell-free culture (Bacillus licheniformis) were used compared to 4. 83mN.m-1 cell-free culture of Bacillus subtilis. As a result, cell-free culture of (Bacillus licheniformis) changes the wettability of the biosurfactant treatment for contact angle measurement to more water-wet as the angle decreased from 130.75o to 65.17o. The influence of microbial treatment on crushed rock samples was also observed by qualitative wettability experiments. Treated samples with biosurfactants remained in the aqueous phase, indicating a water-wet system. These results could prove that biosurfactants can effectively change the chemistry of the wetting conditions against diverse surfaces, providing a desirable condition for efficient oil transport in this way serving as a mechanism for EOR. The environmental friendly effect of biosurfactants applications for industrial purposes play important advantages over chemically synthesized surfactants, with various possible structures, low toxicity, eco-friendly and biodegradability.
Abstract: Fructo-oligosaccharides (FOS) are produced from
sucrose by Aureobasidium pullulans in yields between 40-60%
(w/w). To increase the amount of FOS it is necessary to remove the
small, non-prebiotic sugars, present. Two methods for producing
high-purity FOS have been developed: the use of microorganisms
able to consume small saccharides; and the use of continuous
chromatography to separate sugars: simulated moving bed (SMB). It
is herein proposed the combination of both methods. The aim of this
study is to optimize the composition of the fermentative broth (in
terms of salts and sugars) that will be further purified by SMB. A
yield of 0.63 gFOS.gSucrose^-1 was obtained with A. pullulans using low
amounts of salts in the initial fermentative broth. By removing the
small sugars, Saccharomyces cerevisiae and Zymomonas mobilis
increased the percentage of FOS from around 56.0% to 83% (w/w) in
average, losing only 10% (w/w) of FOS during the recovery process.
Abstract: In this research, the main aim is to investigate the
antimicrobial effectiveness of ammonyx solutions finishing on
Sweatshirt Sport with immersion method. 60 Male healthy subjects
(football player) participated in this study. They were dressed in a
Sweatshirt for 14 days and some microbes found on them were
investigated. The antimicrobial effect of different ammonyx
solutions(1/100, 1/500, 1/1000, 1/2000 v/v solutions of Ammonyx)
on the identified microbes was studied by the zone inhabitation
method in vitro. In the next step the Sweatshirt Sports were treated
with the same different solutions of ammonyx and the antimicrobial
effectiveness was assessed by colony count method in different times
and the results were compared whit untreated ones. Some mechanical
properties of treated cotton/polyester yarn that used in Sweatshirt
Sport were measured after 30 days and were compared with
untreated one. Finally after finishing, scanning electron microscopy
(SEM) was used to compare the surfaces of the finished and
unfinished specimens. The results showed the presence of five
pathogenic microbes on Sweatshirt Sports such as Escherichia coli,
Staphylococcus aureus, Aspergillus, Mucor and Candida. The
inhalation time for treated on Sweatshirt Sports improved. The
amount of colony growth on treated clothes reduced considerably
and moreover the mechanical tests results showed no significant
deterioration effect of studies properties in comparison to the
untreated yarn. The visual examination of the SEM indicated that the
antimicrobial treatments were applied usefully to fabrics.