Abstract: There has been significant recent interest in on-line learning, as well as considerable work on developing technologies for virtual laboratories for engineering students. After reviewing the state-of-the-art of virtual laboratories, this paper steps back from the technology issues to look in more detail at the pedagogical issues surrounding virtual laboratories, and examines the role of gathering student feedback in the development of such laboratories. The main contribution of the paper is a set of student surveys before and after a prototype deployment of a simulation laboratory tool, and the resulting analysis which leads to some tentative guidelines for the design of virtual engineering laboratories.
Abstract: Due to the high reliability reached by DNA tests, since the 1980s this kind of test has allowed the identification of a growing number of criminal cases, including old cases that were unsolved, now having a chance to be solved with this technology. Currently, the use of genetic profiling databases is a typical method to increase the scope of genetic comparison. Forensic laboratories must process, analyze, and generate genetic profiles of a growing number of samples, which require time and great storage capacity. Therefore, it is essential to develop methodologies capable to organize and minimize the spent time for both biological sample processing and analysis of genetic profiles, using software tools. Thus, the present work aims the development of a software system solution for laboratories of forensics genetics, which allows sample, criminal case and local database management, minimizing the time spent in the workflow and helps to compare genetic profiles. For the development of this software system, all data related to the storage and processing of samples, workflows and requirements that incorporate the system have been considered. The system uses the following software languages: HTML, CSS, and JavaScript in Web technology, with NodeJS platform as server, which has great efficiency in the input and output of data. In addition, the data are stored in a relational database (MySQL), which is free, allowing a better acceptance for users. The software system here developed allows more agility to the workflow and analysis of samples, contributing to the rapid insertion of the genetic profiles in the national database and to increase resolution of crimes. The next step of this research is its validation, in order to operate in accordance with current Brazilian national legislation.
Abstract: The teaching of physics in Brazilian public schools emphasizes strongly the theoretical aspects of this science, showing its philosophical and mathematical basis, but neglecting its experimental character. Perhaps the lack of science laboratories explains this practice. In this work, we present a method of teaching physics using the computer. As alternatives to real experiments, we have the trials through simulators, many of which are free software available on the internet. In order to develop a study on the use of simulators in teaching, knowing the impossibility of simulations on all topics in a given subject, we combined these programs with phenomenological and/or experimental texts in order to mitigate this limitation. This study proposes the use of simulators and the debate using phenomenological/experimental texts on electrostatic theme in groups of the 3rd year of EJA (Adult and Youth Education) in order to verify the advantages of this methodology. Some benefits of the hybridization of the traditional method with the tools used were: Greater motivation of the students in learning, development of experimental notions, proactive socialization to learning, greater easiness to understand some concepts and the creation of collaborative activities that can reduce timidity of part of the students.
Abstract: The failure of bridges due to excessive local scour during floods poses a challenging problem to hydraulic engineers. The failure of bridges piers is due to many reasons such as localized scour combined with general riverbed degradation. In this paper, we try to estimate the temporal variation of scour depth at nonuniform cylindrical bridge pier, by experimental work conducted in hydraulic laboratories of Gaziantep University Civil Engineering Department on a flume having dimensions of 8.3 m length, 0.8 m width and 0.9 m depth. The experiments will be carried on 20 cm depth of sediment layer having d50=0.4 mm. Three bridge pier shapes having different scaled models will be constructed in a 1.5m of test section in the channel.
Abstract: Performing culture and characterization of mycobacteria in low resource settings like Nigeria is a very difficult task to undertake because of the very few and limited laboratories carrying out such an experiment; this is a largely due to stringent and laborious nature of the tests. Hence, a rapid, simple and accurate test for characterization is needed. The “SD BIOLINE TB Ag MPT 64 Rapid ®” is a simple and rapid immunochromatographic test used in differentiating Mycobacteria into Mycobacterium tuberculosis (NTM). The 100 sputa were obtained from patients suspected to be infected with tuberculosis and presented themselves to hospitals for check-up and treatment were involved in the study. The samples were cultured in a class III Biosafety cabinet and level III biosafety practices were followed. Forty isolates were obtained from the cultured sputa, and there were identified as Acid-fast bacilli (AFB) using Zeihl-Neelsen acid-fast stain. All the isolates (AFB positive) were then subjected to the SD BIOLINE Analyses. A total of 31 (77.5%) were characterized as MTBC, while nine (22.5%) were NTM. The total turnaround time for the rapid assay was just 30 minutes as compared to a few days of phenotypic and genotypic method. It was simple, rapid and reliable test to differentiate MTBC from NTM.
Abstract: The purpose of this study was to investigate the performance of chemistry in Zanzibar Secondary Schools. It was conducted in all regions of Zanzibar in public and private secondary schools and Ministry of Education officials. The objective of the study included finding out causes of poor performance in chemistry. Views, opinions, and suggestions of teachers and students to improve performance of chemistry and a descriptive survey was adopted for the study. 45 teachers and 200 students were randomly sampled from 15 secondary schools in Zanzibar and ten Ministry of Education officials were purposively sampled for the study. Questionnaires and open-ended interview schedules were the main instruments used in obtaining relevant data from respondents. Data collected from the field was analyzed both qualitatively and quantitatively. Qualitative analysis involved content analysis of the responses obtained through interviews and quantitative analysis involved generation of tables, frequencies and percentages. The results revealed that there were shortages of trained teachers, lack of proficiency in the language of instruction (English) and major facilities like laboratories and books. These led to poor delivery of subject matter and consequently resulting in poor performance. Based on the findings, this study recommends that provision of trained, competent, and effective teachers as vital aspects to be considered. Government through Ministry of Education should put effort to stalk libraries and equip laboratories with modern books and instruments. In addition, the ministry should strengthen teachers’ training and encourage use of instructional media in class and make conducive learning environment to both teachers and students.
Abstract: A challenge for laboratories is to provide bacterial identification and antibiotic sensitivity results within a short time. Hence, advancement in the required technology is desirable to improve timing, accuracy and quality. Even with the current advances in methods used for both phenotypic and genotypic identification of bacteria the need is there to develop method(s) that enhance the outcome of bacteriology laboratories in accuracy and time. The hypothesis introduced here is based on the assumption that the chromosome of any bacteria contains unique sequences that can be used for its identification and typing. The outcome of a pilot study designed to test this hypothesis is reported in this manuscript. Methods: The complete chromosome sequences of several bacterial species were downloaded to use as search targets for unique sequences. Visual basic and SQL server (2014) were used to generate a complete set of 18-base long primers, a process started with reverse translation of randomly chosen 6 amino acids to limit the number of the generated primers. In addition, the software used to scan the downloaded chromosomes using the generated primers for similarities was designed, and the resulting hits were classified according to the number of similar chromosomal sequences, i.e., unique or otherwise. Results: All primers that had identical/similar sequences in the selected genome sequence(s) were classified according to the number of hits in the chromosomes search. Those that were identical to a single site on a single bacterial chromosome were referred to as unique. On the other hand, most generated primers sequences were identical to multiple sites on a single or multiple chromosomes. Following scanning, the generated primers were classified based on ability to differentiate between medically important bacterial and the initial results looks promising. Conclusion: A simple strategy that started by generating primers was introduced; the primers were used to screen bacterial genomes for match. Primer(s) that were uniquely identical to specific DNA sequence on a specific bacterial chromosome were selected. The identified unique sequence can be used in different molecular diagnostic techniques, possibly to identify bacteria. In addition, a single primer that can identify multiple sites in a single chromosome can be exploited for region or genome identification. Although genomes sequences draft of isolates of organism DNA enable high throughput primer design using alignment strategy, and this enhances diagnostic performance in comparison to traditional molecular assays. In this method the generated primers can be used to identify an organism before the draft sequence is completed. In addition, the generated primers can be used to build a bank for easy access of the primers that can be used to identify bacteria.
Abstract: Extrapolation chambers were designed to be used as primary standard dosimeter for measuring absorbed dose in a medium in beta radiation and low energy x-rays. The International Organization for Standardization established series of reference x-radiation for calibrating and determining the energy dependence of dosimeters that are to be reproduced in metrology laboratories. Standardization of the low energy x-ray beams with tube potential lower than 30 kV may be affected by the instrument used for dosimetry. In this work, parameters of a 23392 model PTW extrapolation chamber were determined aiming its use in low energy x-ray beams as a reference instrument.
Abstract: In patients with acute lymphoblastic leukaemia (ALL), treatment response is increasingly evaluated with minimal residual disease (MRD) analyses. Flow Cytometry (FCM) is a fast and sensitive method to detect MRD. However, the interpretation of these multi-parametric data requires intensive operator training and experience. This paper presents a pipeline-software, as a ready-to-use FCM-based MRD-assessment tool for the daily clinical practice for patients with ALL. The new tool increases accuracy in assessment of FCM-MRD in samples which are difficult to analyse by conventional operator-based gating since computer-aided analysis potentially has a superior resolution due to utilization of the whole multi-parametric FCM-data space at once instead of step-wise, two-dimensional plot-based visualization. The system developed as a telemedical network reduces the work-load and lab-costs, staff-time needed for training, continuous quality control, operator-based data interpretation. It allows dissemination of automated FCM-MRD analysis to medical centres which have no established expertise for the benefit of an even larger community of diseased children worldwide. We established a telemedical network system for analysis and clinical follow-up and treatment monitoring of Leukaemia. The system is scalable and adapted to link several centres and laboratories worldwide.
Abstract: Cybersecurity is one of the greatest challenges society faces in an age revolving around technological development. With cyber-attacks on the continuous rise, the nation needs to understand and learn ways that can prevent such attacks. A major contribution that can change the education system is to implement laboratories and competitions into academia. This method can improve and educate students with more hands-on exercises in a highly motivating setting. Considering the fact that students are the next generation of the nation’s workforce, it is important for students to understand concepts not only through books, but also through actual hands-on experiences in order for them to be prepared for the workforce. An effective cybersecurity education system is critical for creating a strong cyber secure workforce today and for the future. This paper emphasizes the need for awareness and the need for competitions and cybersecurity laboratories to be implemented into the education system.
Abstract: This paper details the progress made in the development of the different state-of-the-art aerodynamic tools for the analysis of vertical axis wind turbines including the flow simulation around the blade, viscous flow, stochastic wind, and dynamic stall effects. The paper highlights the capabilities of the developed wind turbine aerodynamic codes over the last thirty years which are currently being used in North America and Europe by Sandia Laboratories, FloWind, IMST Marseilles, and Hydro-Quebec among others. The aerodynamic codes developed at Ecole Polytechnique de Montreal, Canada, represent valuable tools for simulating the flow around wind turbines including secondary effects. Comparison of theoretical results with experimental data have shown good agreement. The strength of the aerodynamic codes based on Double-Multiple Stream tube model (DMS) lies in its simplicity, accuracy, and ability to analyze secondary effects that interfere with wind turbine aerodynamic calculations.
Abstract: Selenium is an-antioxidant which is important for
human health enters food chain through crops. In Kenya Zea mays is
consumed by 96% of population hence is a cheap and convenient
method to provide selenium to large number of population. Several
soil factors are known to have antagonistic effects on selenium
speciation hence the uptake by Zea mays. There are no studies in
Kenya that has been done to determine the effects of soil
characteristics (pH, Tcarbon, CEC, Eh) affect accumulation of
selenium in Zea mays grains in Maize Belt in Kenya. About 100 Zea mays grain samples together with 100 soil samples
were collected from the study site put in separate labeled Ziplocs and
were transported to laboratories at room temperature for analysis.
Maize grains were analyzed for selenium while soil samples were
analyzed for pH, Cat Ion Exchange Capacity, total carbon, and
electrical conductivity. The mean selenium in Zea mays grains varied from 1.82 ± 0.76
mg/Kg to 11±0.86 mg/Kg. There was no significant difference
between selenium levels between different grain batches {χ (Df =76)
= 26.04 P= 1.00} The pH levels varied from 5.43± 0.58 to 5.85±
0.32. No significant correlations between selenium in grains and soil
pH (Pearson’s correlations = - 0.143), and between selenium levels in
grains and the four (pH, Tcarbon, CEC, Eh) soil chemical
characteristics {F (4,91) = 0.721 p = 0.579} was observed. It can be concluded that the soil chemical characteristics in the
study site did not significantly affect the accumulation of native
selenium in Zea mays grains.
Abstract: Typical load-bearing biological materials like bone,
mineralized tendon and shell, are biocomposites made from both
organic (collagen) and inorganic (biomineral) materials. This
amazing class of materials with intrinsic internally designed
hierarchical structures show superior mechanical properties with
regard to their weak components from which they are formed.
Extensive investigations concentrating on static loading conditions
have been done to study the biological materials failure. However,
most of the damage and failure mechanisms in load-bearing
biological materials will occur whenever their structures are exposed
to dynamic loading conditions. The main question needed to be
answered here is: What is the relation between the layout and
architecture of the load-bearing biological materials and their
dynamic behavior? In this work, a staggered model has been
developed based on the structure of natural materials at nanoscale and
Finite Element Analysis (FEA) has been used to study the dynamic
behavior of the structure of load-bearing biological materials to
answer why the staggered arrangement has been selected by nature to
make the nanocomposite structure of most of the biological materials.
The results showed that the staggered structures will efficiently
attenuate the stress wave rather than the layered structure.
Furthermore, such staggered architecture is effectively in charge of
utilizing the capacity of the biostructure to resist both normal and
shear loads. In this work, the geometrical parameters of the model
like the thickness and aspect ratio of the mineral inclusions selected
from the typical range of the experimentally observed feature sizes
and layout dimensions of the biological materials such as bone and
mineralized tendon. Furthermore, the numerical results validated with
existing theoretical solutions. Findings of the present work emphasize
on the significant effects of dynamic behavior on the natural
evolution of load-bearing biological materials and can help scientists
to design bioinspired materials in the laboratories.
Abstract: Brass terminal, one of the several crude oil and
petroleum products storage/handling facilities in the Niger Delta was
built in the 1980s. Activities at this site, over the years, released
crude oil into this 3 m-deep, 1500 m-long canal lying adjacent to the
terminal with oil floating on it and its sediment heavily polluted. To
ensure effective clean-up, three major activities were planned: site
characterization, bioremediation pilot plant construction and testing
and full-scale bioremediation of contaminated sediment / bank soil by
land farming. The canal was delineated into 12 lots and each
characterized, with reference to the floating oily phase, contaminated
sediment and canal bank soil. As a result of site characterization, a
pilot plant for on-site bioremediation was designed and a treatment
basin constructed for carrying out pilot bioremediation test.
Following a designed sampling protocol, samples from this pilot
plant were collected for analysis at two laboratories as a quality
assurance / quality control check. Results showed that Brass Canal
upstream is contaminated with dark, thick and viscous oily film with
characteristic hydrocarbon smell while downstream, thin oily film
interspersed with water was observed. Sediments were observed to be
dark with mixture of brownish sandy soil with TPH ranging from
17,800 mg/kg in Lot 1 to 88,500 mg/kg in Lot 12 samples. Brass
Canal bank soil was observed to be sandy from ground surface to 3m,
below ground surface (bgs) it was silty-sandy and brownish while
subsurface soil (4-10m bgs) was sandy-clayey and whitish/grayish
with typical hydrocarbon smell. Preliminary results obtained so far
have been very promising but were proprietary. This project is
considered, to the best of technical literature knowledge, the first
large-scale on-site bioremediation project in the Niger Delta region,
Nigeria.
Abstract: Durian is the flagship fruit of Mindanao and there is
an abundance of several cultivars with many confusing identities/
names.
The project was conducted to develop procedure for reliable and
rapid detection and sorting of durian planting materials. Moreover, it
is also aimed to establish specific genetic or DNA markers for routine
testing and authentication of durian cultivars in question.
The project developed molecular procedures for routine testing.
SSR primers were also screened and identified for their utility in
discriminating durian cultivars collected.
Results of the study showed the following accomplishments:
1. Twenty (29) SSR primers were selected and identified based on
their ability to discriminate durian cultivars,
2. Optimized and established standard procedure for identification
and authentication of Durian cultivars
3. Genetic profile of durian is now available at Biotech Unit
Our results demonstrate the relevance of using molecular
techniques in evaluating and identifying durian clones. The most
polymorphic primers tested in this study could be useful tools for
detecting variation even at the early stage of the plant especially for
commercial purposes. The process developed combines the efficiency
of the microsatellites development process with the optimization of
non-radioactive detection process resulting in a user-friendly protocol
that can be performed in two (2) weeks and easily incorporated into
laboratories about to start microsatellite development projects. This
can be of great importance to extend microsatellite analyses to other
crop species where minimal genetic information is currently
available. With this, the University can now be a service laboratory
for routine testing and authentication of durian clones.
Abstract: Human leukocyte antigen (HLA) typing from next
generation sequencing (NGS) data has the potential for applications in
clinical laboratories and population genetic studies. Here we introduce
a novel technique for HLA typing from NGS data based on
read-mapping using a comprehensive reference panel containing all
known HLA alleles and de novo assembly of the gene-specific short
reads. An accurate HLA typing at high-digit resolution was achieved
when it was tested on publicly available NGS data, outperforming
other newly-developed tools such as HLAminer and PHLAT.
Abstract: Mastitis is one of the most economic disease affecting dairy cows worldwide. Its classic diagnosis using bacterial culture and biochemical findings is a difficult and prolonged method. In this research, using of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) permitted identification of different microorganisms with high accuracy and rapidity (only 24 hours for microbial growth and analysis). During the application of MALDI-TOF MS, one hundred twenty strains of Staphylococcus and Streptococcus species isolated from milk of cows affected by clinical and subclinical mastitis were identified, and the results were compared with those obtained by traditional methods as API and VITEK 2 Systems. 37 of totality 39 strains (~95%) of Staphylococcus aureus (S. aureus) were exactly detected by MALDI TOF MS and then confirmed by a nuc-based PCR technique, whereas accurate identification was observed in 100% (50 isolates) of the coagulase negative staphylococci (CNS) and Streptococcus agalactiae (31 isolates). In brief, our results demonstrated that MALDI-TOF MS is a fast and truthful technique which has the capability to replace conventional identification of several bacterial strains usually isolated in clinical laboratories of microbiology.
Abstract: It is well documented that introductory computer
programming courses are difficult and that failure rates are high. The
aim of this project was to reduce the high failure and withdrawal rates
in learning to program. This paper presents a number of changes in
module organization and instructional delivery system in teaching
CS1. Daily out of class help sessions and tutoring services were
applied, interactive lectures and laboratories, online resources, and
timely feedback were introduced. Five years of data of 563 students
in 21 sections was collected and analyzed. The primary results show
that the failure and withdrawal rates were cut by more than half.
Student surveys indicate a positive evaluation of the modified
instructional approach, overall satisfaction with the course and
consequently, higher success and retention rates.
Abstract: Organic solvents find various applications in many industrial sectors and laboratories as dilution solvents, dispersion solvents, cleaners and even lubricants. Millions of tons of spent organic solvents (SOS) are generated each year worldwide, prompting the need for more efficient, cleaner and safer methods for the treatment and resource recovery of SOS. As a result, acetone, selected as a model compound for SOS, was gasified in supercritical water to assess the feasibility of resource recovery of SOS by means of supercritical water processes. Experiments were conducted with an autoclave reactor. Gaseous product is mainly consists of H2, CO, CO2 and CH4. The effects of three major operating parameters, the reaction temperature, from 673 to 773K, the dosage of oxidizing agent, from 0.3 to 0.5 stoichiometric oxygen, and the concentration of acetone in the feed, 0.1 and 0.2M, on the product gas composition, yield and heating value were evaluated with the water density fixed at about 0.188g/ml.
Abstract: Quick and qualitative services are not new issues anymore in Logistics but those are still important problems along with cost-cutting. Related to the issues, though advanced information technologies are introduced unceasingly from laboratories, yet there is long way to go for smooth and seamless information flows on physical distribution processes in the industrial field. For the reason, the study aims to seek an advanced information delivery and management strategy through an in-depth case study of a Korea intermodal transportation company. It provides an industrial reference and a way to improve the endemic problems of logistics information systems.