Abstract: The grey oyster mushroom, Pleurotus sajor-caju
(PSC), is a common edible mushroom and is now grown
commercially around the world for food. This fungus has been
broadly used as food or food ingredients in various food products for
a long time. To enhance the nutritional quality and sensory attributes
of bakery-based products, PSC powder is used in the present study to
partially replace wheat flour in baked product formulations. The
nutrient content and sensory properties of rice-porridge and
unleavened bread (paratha) incorporated with various levels of PSC
powder were studied. These food items were formulated with either
0%, 2%, 4% or 6% of PSC powder. Results show PSC powder
recorded β-glucan at 3.57g/100g. In sensory evaluation, consumers
gave higher score to both rice-porridge and paratha bread containing
2-4% PSC compared to those that are not added with PSC powder.
The paratha containing 4% PSC powder can be formulated with the
intention in improving overall acceptability of paratha bread.
Meanwhile, for rice-porridge, consumers prefer the formulated
product added with 4% PSC powder. In conclusion, the addition of
PSC powder to partially wheat flour can be recommended for the
purpose of enhancing nutritional composition and maintaining the
acceptability of carbohydrate-based products.
Abstract: Grey mold on grape is caused by the fungus Botrytis
cinerea Pers. Trichodex WP, a new biofungicide, that contains fungal
spores of Trichoderma harzianum Rifai, was used for biological
control of Grey mold on grape. The efficacy of Trichodex WP has
been reported from many experiments. Experiments were carried out
in the locality – Banatski Karlovac, on grapevine species – talijanski
rizling. The trials were set according to instructions of methods
PP1/152(2) and PP1/17(3) , according to a fully randomized block
design. Phytotoxicity was estimated by PP methods 1/135(2), the
intensity of infection according to Towsend Heuberger , the
efficiency by Abbott, the analysis of variance with Duncan test and
PP/181(2). Application of Trichodex WP is limited to the first two
treatments. Other treatments are performed with the fungicides based
on a.i. procymidone, vinclozoline and iprodione.
Abstract: Different agricultural waste peels were assessed for
their suitability to be used as primary substrates for the
bioremediation of free cyanide (CN-) by a cyanide-degrading fungus
Aspergillus awamori isolated from cyanide containing wastewater.
The bioremediated CN- concentration were in the range of 36 to 110
mg CN-/L, with Orange (C. sinensis) > Carrot (D. carota) > Onion
(A. cepa) > Apple (M. pumila), being chosen as suitable substrates
for large scale CN- degradation processes due to: 1) the high
concentration of bioremediated CN-, 2) total reduced sugars released
into solution to sustain the biocatalyst, and 3) minimal residual NH4-
N concentration after fermentation. The bioremediation rate constants
(k) were 0.017h-1 (0h < t < 24h), with improved bioremediation rates
(0.02189h-1) observed after 24h. The averaged nitrilase activity was
~10 U/L.
Abstract: The quality of Ribbed Smoked Sheets
(RSS) primarily based on color, dryness, and the presence or
absence of fungus and bubbles. This quality is strongly
influenced by the drying and fumigation process namely
smoking process. Smoking that is held in high temperature
long time will result scorched dark brown sheets, whereas if
the temperature is too low or slow drying rate would resulted
in less mature sheets and growth of fungus. Therefore need to
find the time and temperature for optimum quality of sheets.
Enhance, unmonitored heat and mass transfer during smoking
process lead to high losses of energy balance. This research
aims to generate simple empirical mathematical model
describing the effect of smoking time and temperature to RSS
quality of color, water content, fungus and bubbles. The
second goal of study was to analyze energy balance during
smoking process. Experimental study was conducted by
measuring temperature, residence time and quality parameters
of 16 sheets sample in smoking rooms. Data for energy
consumption balance such as mass of fuel wood, mass of
sheets being smoked, construction temperature, ambient
temperature and relative humidity were taken directly along
the smoking process. It was found that mathematical model
correlating smoking temperature and time with color is Color
= -169 - 0.184 T4 - 0.193 T3 - 0.160 0.405 T1 + T2 + 0.388 t1
+3.11 t2 + 3.92t3 + 0.215 t4 with R square 50.8% and with
moisture is Moisture = -1.40-0.00123 T4 + 0.00032 T3 +
0.00260 T2 - 0.00292 T1 - 0.0105 t1 + 0.0290 t2 + 0.0452 t3
+ 0.00061 t4 with R square of 49.9%. Smoking room energy
analysis found useful energy was 27.8%. The energy stored in
the material construction 7.3%. Lost of energy in conversion
of wood combustion, ventilation and others were 16.6%. The
energy flowed out through the contact of material construction
with the ambient air was found to be the highest contribution
to energy losses, it reached 48.3%.
Abstract: The complex structure of lignocellulose leads to great
difficulties in converting it to fermentable sugars for the ethanol
production. The major hydrolysis impediments are the crystallinity of
cellulose and the lignin content. To improve the efficiency of
enzymatic hydrolysis, microbial pretreatment of corncob was
investigated using two bacterial strains of Bacillus subtilis A 002 and
Cellulomonas sp. TISTR 784 (expected to break open the crystalline
part of cellulose) and lignin-degrading fungus, Phanerochaete
sordida SK7 (expected to remove lignin from lignocellulose). The
microbial pretreatment was carried out with each strain under its
optimum conditions. The pretreated corncob samples were further
hydrolyzed to produce reducing glucose with low amounts of
commercial cellulase (25 U·g-1 corncob) from Aspergillus niger. The
corncob samples were determined for composition change by X-ray
diffraction (XRD), Fourier transform infrared spectroscopy (FTIR),
and scanning electron microscope (SEM). According to the results,
the microbial pretreatment with fungus, P. sordida SK7 was the most
effective for enhancing enzymatic hydrolysis, approximately, 40%
improvement.
Abstract: The cDNA encoding the 326 amino acids of a Class I
basic chitinase gene from Leucaena leucocephala de Wit (KB3,
Genbank accession: AAM49597) was cloned under the control of
CaMV35S promoter in pCAMBIA 1300 and transferred to
Koshihikari. Calli of Koshihikari rice was transformed with
agrobacterium with this construct expressing the chitinase and β-
glucouronidase (GUS). The frequencies of calli 90 % has been
obtained from rice seedlings cultured on NB medium. The high
regeneration frequencies, 74% was obtained from calli cultured on
regeneration medium containing 4 mg/l BAP, and 7 g/l phytagel at
25°C. Various factors were studied in order to establish a procedure
for the transformation of Koshihikari Agrobacterium tumefaciens.
Supplementation of 50 mM acetosyringone to the medium during
coculivation was important to enhance the frequency to transient
transformation. The 4 week-old scutellum-derived calli were
excellent starting materials. Selection medium based on NB medium
supplement with 40 mg/l hygromycin and 400 mg/l cefotaxime were
an optimized medium for selection of transformed rice calli. The
percentage of transformation 70 was obtained. Recombinant calli and
regenerated rice plants were checked the expression of chitinase and
gus by PCR, northern blot gel, southern blot gel, and gus assay.
Chitinase and gus were expressed in all parts of recombinant rice.
The rice line expressing the KB3 chiitnase was more resistant to the
blast fungus Fusarium monoliforme than control line.
Abstract: De novo genome assembly is always fragmented. Assembly fragmentation is more serious using the popular next generation sequencing (NGS) data because NGS sequences are shorter than the traditional Sanger sequences. As the data throughput of NGS is high, the fragmentations in assemblies are usually not the result of missing data. On the contrary, the assembled sequences, called contigs, are often connected to more than one other contigs in a complicated manner, leading to the fragmentations. False connections in such complicated connections between contigs, named a contig graph, are inevitable because of repeats and sequencing/assembly errors. Simplifying a contig graph by removing false connections directly improves genome assembly. In this work, we have developed a tool, SIMGraph, to resolve ambiguous connections between contigs using NGS data. Applying SIMGraph to the assembly of a fungus and a fish genome, we resolved 27.6% and 60.3% ambiguous contig connections, respectively. These results can reduce the experimental efforts in resolving contig connections.