Abstract: Gamboge disorder (GD) or fruit damage by the yellow sap is a major problem in mangosteen. Mangosteen plants varied in the level of GD, from very low or non GD to low, moderate and high GD. However it was difficult to differentiate between GD and non GD plants because evaluation of the disorder is strongly influenced by environment. In this study we investigated the usefulness of primer designed from bioinformatics related to cell wall strength, termed as MCWS, to predict GD. Plant materials used were 28 mangosteen plants selected based on percentage of GD categorized as high, moderate, low and very low or non GD. The result showed that the specific DNA fragments were absent in the high GD accessions. The MCWS marker suggests as a novel polymorphic marker for GD in mangosteen as well as a marker for detect variability in mangosteen as apomictic plant.
Abstract: In this research a comparison between k-epsilon and
LES model for a deoiling hydrocyclone is conducted. Flow field of
hydrocyclone is obtained by three-dimensional simulations with
OpenFOAM code. Potential of prediction for both methods of this
complex swirl flow is discussed. Large eddy simulation method
results have more similarity to experiment and its results are
presented in figures from different hydrocyclone cross sections.
Abstract: In hydrocyclones, the particle separation efficiency is
limited by the suspended fine particles, which are discharged with the
coarse product in the underflow. It is well known that injecting water
in the conical part of the cyclone reduces the fine particle fraction in
the underflow. This paper presents a mathematical model that
simulates the water injection in the conical component. The model
accounts for the fluid flow and the particle motion. Particle
interaction, due to hindered settling caused by increased density and
viscosity of the suspension, and fine particle entrainment by settling
coarse particles are included in the model. Water injection in the
conical part of the hydrocyclone is performed to reduce fine particle
discharge in the underflow. The model demonstrates the impact of
the injection rate, injection velocity, and injection location on the
shape of the partition curve. The simulations are compared with
experimental data of a 50-mm cyclone.
Abstract: Bovine viral diarrhea virus (BVDV) can cause lifelong
persistent infection. One reason for the phenomena is attributed to
BVDV infection to placenta tissue. However the mechanisms that
BVDV invades into placenta tissue remain unclear. To clarify the
molecular mechanisms, we investigated the possible means that
BVDV entered into bovine trophoblast cells (TPC). Yeast two-hybrid
system was used to identify proteins extracted from TPC, which
interact with BVDV envelope glycoprotein E2. A PGbkt7-E2 yeast
expression vector and TPC cDNA library were constructed. Through
two rounds of screening, three positive clones were identified.
Sequencing analysis indicated that all the three positive clones
encoded the same protein clathrin. Physical interaction between
clathrin and BVDV E2 protein was further confirmed by
coimmunoprecipitation experiments. This result suggested that the
clathrin might play a critical role in the process of BVDV entry into
placenta tissue and might be a novel antiviral target for preventing
BVDV infection.
Abstract: Methanol-to-olefins coupled with transformation of
coal or natural gas to methanol gives an interesting and promising way
to produce ethylene and propylene. To investigate solid concentration
in gas-solid fluidized bed for methanol-to-olefins process catalyzed by
SAPO-34, a cold model experiment system is established in this paper.
The system comprises a gas distributor in a 300mm internal diameter
and 5000mm height acrylic column, the fiber optic probe system and
series of cyclones. The experiments are carried out at ambient
conditions and under different superficial gas velocity ranging from
0.3930m/s to 0.7860m/s and different initial bed height ranging from
600mm to 1200mm. The effects of radial distance, axial distance,
superficial gas velocity, initial bed height on solid concentration in the
bed are discussed. The effects of distributor shape and porosity on
solid concentration are also discussed. The time-averaged solid
concentration profiles under different conditions are obtained.
Abstract: This paper describes a code clone visualization method, called FC graph, and the implementation issues. Code clone detection tools usually show the results in a textual representation. If the results are large, it makes a problem to software maintainers with understanding them. One of the approaches to overcome the situation is visualization of code clone detection results. A scatter plot is a popular approach to the visualization. However, it represents only one-to-one correspondence and it is difficult to find correspondence of code clones over multiple files. FC graph represents correspondence among files, code clones and packages in Java. All nodes in FC graph are positioned using force-directed graph layout, which is dynami- cally calculated to adjust the distances of nodes until stabilizing them. We applied FC graph to some open source programs and visualized the results. In the author’s experience, FC graph is helpful to grasp correspondence of code clones over multiple files and also code clones with in a file.
Abstract: Methanol-to-olefins (MTO) coupled with
transformation of coal or natural gas to methanol gives an interesting
and promising way to produce ethylene and propylene. To investigate
solid concentration in gas-solid fluidized bed for methanol-to-olefins
process catalyzed by SAPO-34, a cold model experiment system is
established in this paper. The system comprises a gas distributor in a
300mm internal diameter and 5000mm height acrylic column, the
fiber optic probe system and series of cyclones. The experiments are
carried out at ambient conditions and under different superficial gas
velocity ranging from 0.3930m/s to 0.7860m/s and different initial bed
height ranging from 600mm to 1200mm. The effects of radial
distance, axial distance, superficial gas velocity, initial bed height on
solid concentration in the bed are discussed. The effects of distributor
shape and porosity on solid concentration are also discussed. The
time-averaged solid concentration profiles under different conditions
are obtained.
Abstract: In this research the separation efficiency of deoiling hydrocyclone is evaluated using three-dimensional simulation of multiphase flow based on Eulerian-Eulerian finite volume method. The mixture approach of Reynolds Stress Model is also employed to capture the features of turbulent multiphase swirling flow. The obtained separation efficiency of Colman's design is compared with available experimental data and showed that the separation curve of deoiling hydrocyclones can be predicted using numerical simulation.
Abstract: The project was undertaken to determine the effects of modified tissue culture protocols e.g. age of culture and hormone levels (2,4-D) in generating somaclonal variation. Moreover, the utility of molecular markers (SSR and MSAP) in sorting off types/somaclones were investigated.
Results show that somaclonal variation is in effect due to prolonged subculture and high 2,4-D concentration. The resultant variation was observed to be due to high level of methylation events specifically cytosine methylation either at the internal or external cytosine and was identified by methylation sensitive amplification polymorphism (MSAP).Simple sequence repeats (SSR) on the other hand, was able to associate a marker to a trait of interest.
These therefore, show that molecular markers can be an important tool in sorting out variation/mutants at an early stage.
Abstract: Tofurther advance research on immune-related genes
from T. molitor, we constructed acDNA library and analyzed
expressed sequence taq (EST) sequences from 1,056 clones. After
removing vector sequence and quality checkingthrough thePhred
program (trim_alt 0.05 (P-score>20), 1039 sequences were generated.
The average length of insert was 792 bp. In addition, we identified 162
clusters, 167 contigs and 391 contigs after clustering and assembling
process using a TGICL package. EST sequences were searchedagainst
NCBI nr database by local BLAST (blastx, E
Abstract: This paper presents the results of preliminary
assessment of water quality along the coastal areas in the vicinity of
Left Bank Outfall Drainage (LBOD) and Tidal Link Drain (TLD) in
Sindh province after the cyclone 2A occurred in 1999. The water
samples were collected from various RDs of Tidal Link Drain and
lakes during September 2001 to April 2002 and were analysed for
salinity, nitrite, phosphate, ammonia, silicate and suspended material
in water. The results of the study showed considerable variations in
water quality depending upon the location along the coast in the
vicinity of LBOD and RDs. The salinity ranged between 4.39–65.25
ppt in Tidal Link Drain samples whereas 2.4–38.05 ppt in samples
collected from lakes. The values of suspended material at various
RDs of Tidal Link Drain ranged between 56.6–2134 ppm and at the
lakes between 68–297 ppm. The data of continuous monitoring at
RD–93 showed the range of PO4 (8.6–25.2 μg/l), SiO3 (554.96–1462
μg/l), NO2 (0.557.2–25.2 μg/l) and NH3 (9.38–23.62 μg/l). The
concentration of nutrients in water samples collected from different
RDs was found in the range of PO4 (10.85 to 11.47 μg/l), SiO3 (1624
to 2635.08 μg/l), NO2 (20.38 to 44.8 μg/l) and NH3 (24.08 to 26.6
μg/l). Sindh coastal areas which situated at the north-western
boundary the Arabian Sea are highly vulnerable to flood damages
due to flash floods during SW monsoon or impact of sea level rise
and storm surges coupled with cyclones passing through Arabian Sea
along Pakistan coast. It is hoped that the obtained data in this study
would act as a database for future investigations and monitoring of
LBOD and Tidal Link Drain coastal waters.