Abstract: The textile industry plays a major role in the economy
of India and on the other side of the coin it is the major source for
water pollution. As azo dyes is the largest dye class they are
extensively used in many fields such as textile industry, leather
tanning industry, paper production, food, color photography,
pharmaceuticals and medicine, cosmetic, hair colorings, wood
staining, agricultural, biological and chemical research etc. In
addition to these, they can have acute and/or chronic effects on
organisms depending on their concentration and length of exposure
when they discharged as effluent in the environment. The aim of this
study was to assess the genotoxic and histotoxic potentials of
environmentally relevant concentrations of C. I. Reactive Red 120
(RR 120) on Catla catla, important edible freshwater fingerlings. For
this, healthy Catla catla fingerlings were procured from the
Government Fish Farm and acclimatized in 100 L capacity and
continuously aerated glass aquarium in laboratory for 15 days.
According to APHA some physic-chemical parameters were
measured and maintained such as temperature, pH, dissolve oxygen,
alkalinity, total hardness. Water along with excreta had been changed
every 24 hrs. All fingerlings were fed artificial food palates once a
day @ body weight. After 15 days fingerlings were grouped in 5 (10
in each) and exposed to various concentrations of RR 120 (Control,
10, 20, 30 and 40 mg.l-1) and samples (peripheral blood and gills,
kidney) were collected and analyzed at 96 hrs. All results were
compared with the control. Micronuclei (MN), nuclear buds (NB),
fragmented-apoptotic (FA) and bi-nucleated (BN) cells in blood
smears and in tissues (gills and kidney cells) were observed.
Prominent histopathological alterations were noticed in gills such as
aneurism, hyperplasia, degenerated central axis, lifting of gill
epithelium, curved secondary gill lamellae etc. Similarly kidney
showed some detrimental changes like shrunken glomeruli with
increased periglomerular space, degenerated renal tubules etc. Both
haematological and histopathological changes clearly reveal the toxic
potential of RR 120. This work concludes that water pollution
assessment can be done by these two biomarkers which provide
baseline to the further chromosomal or molecular work.
Abstract: This research has been conducted to study the method
of culture and comparing growth and biomass of Gracilaria corticata
cultured on rope and net for 50 days through two treatments (first
treatment: culture of alga on net and the second treatment: culture of
alga on rope and each treatment was repeated by four cases). During
culture period, the water of aquariums was replaced once every two
days for 40-50%. Also, 0.3-0.5 grams of urea fertilizer was added to
the culture environment for fertilization. Moreover, some of the
environmental factors such as pH, salinity and temperature of the
environment were measured on a daily basis. During the culture
period, extent of longitudinal growth of the species of both treatments
was equal. The said length was reached from 8-10 cm to 10.5-13 cm
accordingly. The resulted weight in repetitions of the first treatment
was higher than that of the second treatment in such a way as in the
first treatment, its weight reached from 10 grams to 21.119 grams and
in the second treatment, its weight reached from 10 grams to 17.663
grams. On a whole, it may be stated that that kind of alga being
studied has a considerable growth with respect to its volume. The
results have revealed that the percentage of daily growth and wet
weight at the end of the first treatment was higher than that of the
second treatment and it was registered as 0.934, 6.072 and 811.432 in
the first treatment and 0.797, 4.990 and 758.071 in the second
treatment respectively. This difference is significant (P
Abstract: There is an essential need for obtaining the mathematical representation of fish body undulations, which can be used for designing and building new innovative types of marine propulsion systems with less environmental impact. This research work presents a case study to derive the mathematical model for fish body movement. Observation and capturing image methods were used in this study in order to obtain a mathematical representation of Clariasbatrachus fish (catfish). An experiment was conducted by using an aquarium with dimension 0.609 m x 0.304 m x 0.304 m, and a 0.5 m ruler was attached at the base of the aquarium. Progressive Scan Monochrome Camera was positioned at 1.8 m above the base of the aquarium to provide swimming sequences. Seven points were marked on the fish body using white marker to indicate the fish movement and measuring the amplitude of undulation. Images from video recordings (20 frames/s) were analyzed frame by frame using local coordinate system, with time interval 0.05 s. The amplitudes of undulations were obtained for image analysis from each point that has been marked on fish body. A graph of amplitude of undulations versus time was plotted by using computer to derive a mathematical fit. The function for the graph is polynomial with nine orders.
Abstract: Fishes, in general, follow ammonotelic mode of excretion. However, certain stress factors may provoke them to excrete urea. In the present study, the possible role of ureogenesis to avoid accumulation of toxic ammonia under water-restricted condition was tested in Heteropneustes fossilis. A total of hundred fishes were collected and sacrificed. Excretory urea and ammonia were estimated in the water of the aquarium and glutamate dehydrogenase acitivity was measured in the hepatic tissue. During the experimental period, excretory ammonia in Heteropneustes fossilis was found between 931% to 16% above the baseline ammonia and excretory urea was found between 112% to 898% above the baseline urea. A high degree of correlation with r (coefficient of correlation) above 0.9 is observed between excretory ammonia and urea in Heteropneustes fossilis. However, only a moderate degree of correlation is observed between the activity of glutamate dehydrogenase and excretory ammonia and urea.