Abstract: Rapid prototyping (RP) techniques are a group of
advanced manufacturing processes that can produce custom made
objects directly from computer data such as Computer Aided Design
(CAD), Computed Tomography (CT) and Magnetic Resonance
Imaging (MRI) data. Using RP fabrication techniques, constructs
with controllable and complex internal architecture with appropriate
mechanical properties can be achieved. One of the attractive and
promising utilization of RP techniques is related to tissue engineering
(TE) scaffold fabrication. Tissue engineering scaffold is a 3D
construction that acts as a template for tissue regeneration. Although
several conventional techniques such as solvent casting and gas
forming are utilized in scaffold fabrication; these processes show
poor interconnectivity and uncontrollable porosity of the produced
scaffolds. So, RP techniques become the best alternative fabrication
methods of TE scaffolds. This paper reviews the current state of the
art in the area of tissue engineering scaffolds fabrication using
advanced RP processes, as well as the current limitations and future
trends in scaffold fabrication RP techniques.
Abstract: Bionanotechnology deals with nanoscopic interactions between nanostructured materials and biological systems. Polymer nanocomposites with optimized biological activity have attracted great attention. Nanoclay is considered as reinforcing nanofiller in manufacturing of high performance nanocomposites. In current study, organomodified-nanoclay with negatively charged silicate layers was incorporated into biomedical grade silicone rubber. Nanoparticle loading has been tailored to enhance cell behavior. Addition of nanoparticles led to improved mechanical properties of substrate with enhanced strength and stiffness while no toxic effects was observed. Results indicated improved viability and proliferation of cells by addition of nanofillers. The improved mechanical properties of the matrix result in proper cell response through adjustment and arrangement of cytoskeletal fibers. Results can be applied in tissue engineering when enhanced substrates are required for improvement of cell behavior for in vivo applications.