Abstract: Cyanobacteria play a vital role in the production of phycobiliproteins that includes phycocyanin and phycoerythrin pigments. Phycocyanin and related phycobiliproteins have wide variety of application that is used in the food, biotechnology and cosmetic industry because of their color, fluorescent and antioxidant properties. The present study is focused to understand the pigment at molecular level in the Cyanobacteria Oscillatoria terebriformis NTRI05 and Oscillatoria foreaui NTRI06. After extraction of genomic DNA, the amplification of C-Phycocyanin gene was done with the suitable primer PCβF and PCαR and the sequencing was performed. Structural and Phylogenetic analysis was attained using the sequence to develop a molecular model.
Abstract: Thermite welding is mainly used in world. The
reasons why the thermite welding method is widely used are
that the equipment has good mobility and total working time
of that is shorter than that of the enclosed arc welding method
on site. Moreover, the operating skill, which required for
thermite welding, is less than that of for enclosed arc welding.
In the present research work, heat treatment and combined
'expulsion and heat treatment' techniques were used improve
the mechanical properties and weldment structure. The
specimens were cut in the transverse direction from expulsion
with Heat treated and heat treated Thermite Welded rails.
Specimens were prepared according to AWS standard and
subjected to tensile test, Impact test and hardness and their
results were tabulated. Microstructural analysis was carried
out with the help of SEM. Then analyze to effect of heat
treated and 'expulsion with heat treated' with the properties of
their thermite welded rails. Compare the mechanical and
microstructural properties of thermite welded rails between
heat expulsion with heat treated and heat treated. Mechanical
and microstructural response expulsion with heat treated
thermite welded rail is higher value as compared to heat
treatment.
Abstract: We present a preliminary x-ray study on human-hair
microstructures for a health-state indicator, in particular a cancer
case. As an uncomplicated and low-cost method of x-ray technique,
the human-hair microstructure was analyzed by wide-angle x-ray
diffractions (XRD) and small-angle x-ray scattering (SAXS). The
XRD measurements exhibited the simply reflections at the d-spacing
of 28 Å, 9.4 Å and 4.4 Å representing to the periodic distance of the
protein matrix of the human-hair macrofibrous and the diameter and
the repeated spacing of the polypeptide alpha helixes of the
photofibrils of the human-hair microfibrous, respectively. When
compared to the normal cases, the unhealthy cases including to the
breast- and ovarian-cancer cases obtained higher normalized ratios of
the x-ray diffracting peaks of 9.4 Å and 4.4 Å. This likely resulted
from the varied distributions of microstructures by a molecular
alteration. As an elemental analysis by x-ray fluorescence (XRF), the
normalized quantitative ratios of zinc(Zn)/calcium(Ca) and
iron(Fe)/calcium(Ca) were determined. Analogously, both Zn/Ca and
Fe/Ca ratios of the unhealthy cases were obtained higher than both of
the normal cases were. Combining the structural analysis by XRD
measurements and the elemental analysis by XRF measurements
exhibited that the modified fibrous microstructures of hair samples
were in relation to their altered elemental compositions. Therefore,
these microstructural and elemental analyses of hair samples will be
benefit to associate with a diagnosis of cancer and genetic diseases.
This functional method would lower a risk of such diseases by the
early diagnosis. However, the high-intensity x-ray source, the highresolution
x-ray detector, and more hair samples are necessarily
desired to develop this x-ray technique and the efficiency would be
enhanced by including the skin and fingernail samples with the
human-hair analysis.
Abstract: Over the course of the past century, the global
automotive industry-s stance towards safety has evolved from one of
contempt to one nearing reverence. A suspension system that
provides safe handling and cornering capabilities can, with the help
of an efficient braking system, improve safety to a large extent. The
aim of this research is to propose a new automotive brake rotor
design and to compare it with automotive vented disk rotor. Static
structural and transient thermal analysis have been carried out on the
vented disk rotor and proposed rotor designs to evaluate and compare
their performance. Finite element analysis was employed for both
static structural and transient thermal analysis. Structural analysis
was carried out to study the stress and deformation pattern of the
rotors under extreme loads. Time varying temperature load was
applied on the rotors and the temperature distribution was analysed
considering cooling parameters (convection and radiation). This
dissertation illustrates the use of Finite Element Methods to examine
models, concluding with a comparative study of the proposed rotor
design and the conventional vented disk rotor for structural stability
and thermal efficiency.
Abstract: A strategy is implemented to find the improved configuration design of an existing aircraft structure by executing topology and shape optimizations. Structural analysis of the Initial Design Space is performed in ANSYS under the loads pertinent to operating and ground conditions. By using the FEA results and data, an initial optimized layout configuration is attained by exploiting nonparametric topology optimization in TOSCA software. Topological optimized surfaces are then smoothened and imported in ANSYS to develop the geometrical features. Nodes at the critical locations of resulting voids are selected for sketching rough profiles. Rough profiles are further refined and CAD feasible geometric features are generated. The modified model is then analyzed under the same loadings and constraints as defined for topology optimization. Shape at the peak stress concentration areas are further optimized by exploiting the shape optimization in TOSCA.shape module. The harmonized stressed model with the modified surfaces is then imported in CATIA to develop the final design.
Abstract: In this study rack systems that are structural storage
units of warehouses have been analyzed as structural with Finite
Element Method (FEA). Each cell of discussed rack system storages
pallets which have from 800 kg to 1000 kg weights and
0.80x1.15x1.50 m dimensions. Under this load, total deformations
and equivalent stresses of structural elements and principal stresses,
tensile stresses and shear stresses of connection elements have been
analyzed. The results of analyses have been evaluated according to
resistance limits of structural and connection elements. Obtained
results have been presented as visual and magnitude.
Abstract: The main emphasis of metallurgists has been to process the materials to obtain the balanced mechanical properties for the given application. One of the processing routes to alter the properties is heat treatment. Nearly 90% of the structural applications are related to the medium carbon an alloyed steels and hence are regarded as structural steels. The major requirement in the conventional steel is to improve workability, toughness, hardness and grain refinement. In this view, it is proposed to study the mechanical and tribological properties of unalloyed structural (AISI 1140) steel with different thermal (heat) treatments like annealing, normalizing, tempering and hardening and compared with as brought (cold worked) specimen. All heat treatments are carried out in atmospheric condition. Hardening treatment improves hardness of the material, a marginal decrease in hardness value with improved ductility is observed in tempering. Annealing and normalizing improve ductility of the specimen. Normalized specimen shows ultimate ductility. Hardened specimen shows highest wear resistance in the initial period of slide wear where as above 25KM of sliding distance, as brought steel dominates the hardened specimen. Both mild and severe wear regions are observed. Microstructural analysis shows the existence of pearlitic structure in normalized specimen, lath martensitic structure in hardened, pearlitic, ferritic structure in annealed specimen.
Abstract: Biochemical and molecular analysis of some
antioxidant enzyme genes revealed different level of gene expression
on oilseed (Brassica napus). For molecular and biochemical
analysis, leaf tissues were harvested from plants at eight different
developmental stages, from young to senescence. The levels of total
protein and chlorophyll were increased during maturity stages of
plant, while these were decreased during the last stages of plant
growth. Structural analysis (nucleotide and deduced amino acid
sequence, and phylogenic tree) of a complementary DNA revealed a
high level of similarity for a family of Catalase genes. The
expression of the gene encoded by different Catalase isoforms was
assessed during different plant growth phase. No significant
difference between samples was observed, when Catalase activity
was statistically analyzed at different developmental stages. EST
analysis exhibited different transcripts levels for a number of other
relevant antioxidant genes (different isoforms of SOD and
glutathione). The high level of transcription of these genes at
senescence stages was indicated that these genes are senescenceinduced
genes.
Abstract: The aim of this paper is the analysis and preservation of lime kilns, focusing on the structure, construction, and functionality of vertical shaft lime kilns of the Cap Corse in Corsica. Plans and sections of two lime kilns are presented in detail, providing an overall picture of this specific industrial heritage. The potential damage areas are identified performing structural analysis of a lime kiln using the finite element method. A restoration and strengthening technique that satisfies the directions of the Charter of Venice is presented using post-tensioning tendons. Recommendations are given to preserve and promote these important historical structures integrating them into the custom footpath.
Abstract: The present work deals with the structural analysis of
turbine blades and modeling of turbine blades. A common failure
mode for turbine machines is high cycle of fatigue of compressor and
turbine blades due to high dynamic stresses caused by blade vibration
and resonance within the operation range of the machinery. In this
work, proper damping system will be analyzed to reduce the
vibrating blade. The main focus of the work is the modeling of under
platform damper to evaluate the dynamic analysis of turbine-blade
vibrations. The system is analyzed using Bond graph technique. Bond
graph is one of the most convenient ways to represent a system from
the physical aspect in foreground. It has advantage of putting together
multi-energy domains of a system in a single representation in a
unified manner. The bond graph model of dry friction damper is
simulated on SYMBOLS-shakti® software. In this work, the blades
are modeled as Timoshenko beam. Blade Vibrations under different
working conditions are being analyzed numerically.
Abstract: The use of 3D computer-aided design (CAD) models
to support construction project planning has been increasing in the
previous year. 3D CAD models reveal more planning ideas by
visually showing the construction site environment in different stages
of the construction process. Using 3D CAD models together with
scheduling software to prepare construction plan can identify errors
in process sequence and spatial arrangement, which is vital to the
success of a construction project. A number of 4D (3D plus time)
CAD tools has been developed and utilized in different construction
projects due to the awareness of their importance. Virtual prototyping
extends the idea of 4D CAD by integrating more features for
simulating real construction process. Virtual prototyping originates
from the manufacturing industry where production of products such
as cars and airplanes are virtually simulated in computer before they
are built in the factory. Virtual prototyping integrates 3D CAD,
simulation engine, analysis tools (like structural analysis and
collision detection), and knowledgebase to streamline the whole
product design and production process. In this paper, we present the
application of a virtual prototyping software which has been used in
a few construction projects in Hong Kong to support construction
project planning. Specifically, the paper presents an implementation
of virtual prototyping in a residential building project in Hong Kong.
The applicability, difficulties and benefits of construction virtual
prototyping are examined based on this project.
Abstract: In literature, there are metrics for identifying the
quality of reusable components but the framework that makes use of
these metrics to precisely predict reusability of software components
is still need to be worked out. These reusability metrics if identified
in the design phase or even in the coding phase can help us to reduce
the rework by improving quality of reuse of the software component
and hence improve the productivity due to probabilistic increase in
the reuse level. As CK metric suit is most widely used metrics for
extraction of structural features of an object oriented (OO) software;
So, in this study, tuned CK metric suit i.e. WMC, DIT, NOC, CBO
and LCOM, is used to obtain the structural analysis of OO-based
software components. An algorithm has been proposed in which the
inputs can be given to K-Means Clustering system in form of
tuned values of the OO software component and decision tree is
formed for the 10-fold cross validation of data to evaluate the in
terms of linguistic reusability value of the component. The developed
reusability model has produced high precision results as desired.
Abstract: This paper presents the analysis of duct design using
static and dynamic approaches. The static approach is used to find
out applicability between the design and material applied. The
material used in this paper is Thermoplastic Olefins (TPO). For the
dynamic approach, the focusing is only on the CFD simulations. The
fatigue life in this design and material applied also covered.
Abstract: A computational platform is presented in this
contribution. It has been designed as a virtual laboratory to be used
for exploring optimization algorithms in biological problems. This
platform is built on a blackboard-based agent architecture. As a test
case, the version of the platform presented here is devoted to the
study of protein folding, initially with a bead-like description of the
chain and with the widely used model of hydrophobic and polar
residues (HP model). Some details of the platform design are
presented along with its capabilities and also are revised some
explorations of the protein folding problems with different types of
discrete space. It is also shown the capability of the platform to
incorporate specific tools for the structural analysis of the runs in
order to understand and improve the optimization process.
Accordingly, the results obtained demonstrate that the ensemble of
computational tools into a single platform is worthwhile by itself,
since experiments developed on it can be designed to fulfill different
levels of information in a self-consistent fashion. By now, it is being
explored how an experiment design can be useful to create a
computational agent to be included within the platform. These
inclusions of designed agents –or software pieces– are useful for the
better accomplishment of the tasks to be developed by the platform.
Clearly, while the number of agents increases the new version of the
virtual laboratory thus enhances in robustness and functionality.
Abstract: Quantitative methods of economic decision-making as
the methodological base of the so called operational research
represent an important set of tools for managing complex economic
systems,both at the microeconomic level and on the macroeconomic
scale. Mathematical models of controlled and controlling processes
allow, by means of artificial experiments, obtaining information
foroptimalor optimum approaching managerial decision-making.The
quantitative methods of economic decision-making usually include a
methodology known as structural analysis -an analysisof
interdisciplinary production-consumption relations.
Abstract: For fire safety purposes, the fire resistance and the
structural behavior of reinforced concrete members are assessed to
satisfy specific fire performance criteria. The available prescribed
provisions are based on standard fire load. Under various fire
scenarios, engineers are in need of both heat transfer analysis and
structural analysis. For heat transfer analysis, the study proposed a
modified finite difference method to evaluate the temperature profile
within a cross section. The research conducted is limited to concrete
sections exposed to a fire on their one side. The method is based on
the energy conservation principle and a pre-determined power
function of the temperature profile. The power value of 2.7 is found
to be a suitable value for concrete sections. The temperature profiles
of the proposed method are only slightly deviate from those of the
experiment, the FEM and the FDM for various fire loads such as
ASTM E 119, ASTM 1529, BS EN 1991-1-2 and 550 oC. The
proposed method is useful to avoid incontinence of the large matrix
system of the typical finite difference method to solve the
temperature profile. Furthermore, design engineers can simply apply
the proposed method in regular spreadsheet software.
Abstract: Some quality control tools use non metric subjective information coming from experts, who qualify the intensity of relations existing inside processes, but without quantifying them. In this paper we have developed a quality control analytic tool, measuring the impact or strength of the relationship between process operations and product characteristics. The tool includes two models: a qualitative model, allowing relationships description and analysis; and a formal quantitative model, by means of which relationship quantification is achieved. In the first one, concepts from the Graphs Theory were applied to identify those process elements which can be sources of variation, that is, those quality characteristics or operations that have some sort of prelacy over the others and that should become control items. Also the most dependent elements can be identified, that is those elements receiving the effects of elements identified as variation sources. If controls are focused in those dependent elements, efficiency of control is compromised by the fact that we are controlling effects, not causes. The second model applied adapts the multivariate statistical technique of Covariance Structural Analysis. This approach allowed us to quantify the relationships. The computer package LISREL was used to obtain statistics and to validate the model.
Abstract: Interior brick-infill partitions are usually considered as
non-structural components and only their weight is accounted for in
practical structural design. In this study, their effect on the progressive
collapse resistance of an RC building subjected to sudden column loss
is investigated. Three notional column loss conditions with four
different brick-infill locations are considered. Column-loss response
analyses of the RC building with and without brick infills are carried
out. Analysis results indicate that the collapse resistance is only
slightly influenced by the brick infills due to their brittle failure
characteristic. Even so, they may help to reduce the inelastic
displacement response under column loss. For practical engineering, it
is reasonably conservative to only consider the weight of brick-infill
partitions in the structural analysis.
Abstract: The requirement to improve software productivity has
promoted the research on software metric technology. There are
metrics for identifying the quality of reusable components but the
function that makes use of these metrics to find reusability of
software components is still not clear. These metrics if identified in
the design phase or even in the coding phase can help us to reduce the
rework by improving quality of reuse of the component and hence
improve the productivity due to probabilistic increase in the reuse
level. CK metric suit is most widely used metrics for the objectoriented
(OO) software; we critically analyzed the CK metrics, tried
to remove the inconsistencies and devised the framework of metrics
to obtain the structural analysis of OO-based software components.
Neural network can learn new relationships with new input data and
can be used to refine fuzzy rules to create fuzzy adaptive system.
Hence, Neuro-fuzzy inference engine can be used to evaluate the
reusability of OO-based component using its structural attributes as
inputs. In this paper, an algorithm has been proposed in which the
inputs can be given to Neuro-fuzzy system in form of tuned WMC,
DIT, NOC, CBO , LCOM values of the OO software component and
output can be obtained in terms of reusability. The developed
reusability model has produced high precision results as expected by
the human experts.
Abstract: Aurein 1.2 is a 13-residue amphipathic peptide with antibacterial and anticancer activity. Aurein1.2 and its retro analog were synthesized to study the activity of the peptides in relation to their structure. The antibacterial test result showed the retro-analog is inactive. The secondary structural analysis by CD spectra indicated that both of the peptides at TFE/Water adopt alpha-helical conformation. MD simulation was performed on aurein 1.2 and retro-analog in water and TFE in order to analyse the factors that are involved in the activity difference between retro and the native peptide. The simulation results are discussed and validated in the light of experimental data from the CD experiment. Both of the peptides showed a relatively similar pattern for their hydrophobicity, hydrophilicity, solvent accessible surfaces, and solvent accessible hydrophobic surfaces. However, they showed different in directions of dipole moment of peptides. Also, Our results further indicate that the reversion of the amino acid sequence affects flexibility .The data also showed that factors causing structural rigidity may decrease the activity. Consequently, our finding suggests that in the case of sequence-reversed peptide strategy, one has to pay attention to the role of amino acid sequence order in making flexibility and role of dipole moment direction in peptide activity. KeywordsAntimicrobial peptides, retro, molecular dynamic, circular dichroism.