Abstract: Egg yolk oil is a natural source of bioactive
compounds such as unsaturated fatty acids, oil soluble vitamins,
pigments and others. Bioactive compound content in egg yolk oil
depends from its content in eggs, from which oil was extracted. Many
studies show that bioactive compound content in egg is correlated to
the content of these compounds in hen feed, but there is also an
opinion that hen housing systems also have influence on egg
chemical content. The aim of this study was to determine which
factor, laying hen housing system or hen diet, has a primary influence
on bioactive compound content in egg yolk oil. The egg yolk oil was
extracted from eggs obtained from 4 different hen housing systems:
cage, barn and two groups of free range. All hens were fed with
commercially produced compound feed except one group of free
range hens which get free diet – pastured hens. Extracted egg yolk
oils were analyzed for fatty acids, oil soluble vitamins and β-carotene
content. α-tocopherol, ergocalcipherol and polyunsaturated fatty acid
content in egg yolk oil was higher from eggs obtained from all
housing systems where hens were fed with commercial compound
feed. β-carotene and retinol content in egg yolk oils from free range
free diet eggs was significantly (p>0.05) higher that from other eggs
because hens have access to green forage. Hen physical activity in
free range housing systems decreases content of some bioactive
compound in egg yolk oil.
Abstract: Skin aging is a slow multifactorial process influenced
by both internal as well as external factors. Ultra-violet radiations
(UV), diet, smoking and personal habits are the most common
environmental factors that affect skin aging. Fat contents and fibrous
proteins as collagen and elastin are core internal structural
components. The direct influence of UV on elastin integrity and
health is central on aging of skin especially by time. The deposition
of abnormal elastic material is a major marker in a photo-aged skin.
Searching for compounds that may protect against cutaneous photodamage
is exceedingly valued. Retinoids and alpha hydroxy acids
have been endorsed by some researchers as possible candidates for
protecting and or repairing the effect of UV damaged skin. For
consolidating a better system of anti- and protective effects of such
anti-aging agents, we evaluated the combinatory effects of various
dosages of lactic acid and retinol on the dermal fibroblast’s elastin
levels exposed to UV. The UV exposed cells showed significant
reduction in the elastin levels. A combination of drugs with a higher
concentration of lactic acid (30 -35 mM) and a lower concentration of
retinol (10-15mg/mL) showed to work better in maintaining elastin
concentration in UV exposed cells. We assume this preservation
could be the result of increased tropo-elastin gene expression
stimulated by retinol whereas lactic acid probably repaired the UV
irradiated damage by enhancing the amount and integrity of the
elastin fibers.