Abstract: A bio-sensing method, based on the plasmonic property of gold nano-islands, has been developed for detection of exosomes in a clinical setting. The position of the gold plasmon band in the UV-Visible spectrum depends on the size and shape of gold nanoparticles as well as on the surrounding environment. By adsorbing various chemical entities, or binding them, the gold plasmon band will shift toward longer wavelengths and the shift is proportional to the concentration. Exosomes transport cargoes of molecules and genetic materials to proximal and distal cells. Presently, the standard method for their isolation and quantification from body fluids is by ultracentrifugation, not a practical method to be implemented in a clinical setting. Thus, a versatile and cutting-edge platform is required to selectively detect and isolate exosomes for further analysis at clinical level. The new sensing protocol, instead of antibodies, makes use of a specially synthesized polypeptide (Vn96), to capture and quantify the exosomes from different media, by binding the heat shock proteins from exosomes. The protocol has been established and optimized by using a glass substrate, in order to facilitate the next stage, namely the transfer of the protocol to a microfluidic environment. After each step of the protocol, the UV-Vis spectrum was recorded and the position of gold Localized Surface Plasmon Resonance (LSPR) band was measured. The sensing process was modelled, taking into account the characteristics of the nano-island structure, prepared by thermal convection and annealing. The optimal molar ratios of the most important chemical entities, involved in the detection of exosomes were calculated as well. Indeed, it was found that the results of the sensing process depend on the two major steps: the molar ratios of streptavidin to biotin-PEG-Vn96 and, the final step, the capture of exosomes by the biotin-PEG-Vn96 complex. The microfluidic device designed for sensing of exosomes consists of a glass substrate, sealed by a PDMS layer that contains the channel and a collecting chamber. In the device, the solutions of linker, cross-linker, etc., are pumped over the gold nano-islands and an Ocean Optics spectrometer is used to measure the position of the Au plasmon band at each step of the sensing. The experiments have shown that the shift of the Au LSPR band is proportional to the concentration of exosomes and, thereby, exosomes can be accurately quantified. An important advantage of the method is the ability to discriminate between exosomes having different origins.
Abstract: We report the enhancement of Raman scattering
signal by one order of magnitude using photonic nanojet (PNJ) of a
lollipop shaped dielectric microstructure (LSDM) fabricated by a
pulsed CO₂ laser. Here, the PNJ is generated by illuminating sphere
portion of the LSDM with non-resonant laser. Unlike the surface
enhanced Raman scattering (SERS) technique, this technique is
simple, and the obtained results are highly reproducible. In addition,
an efficient technique is proposed to enhance the SERS signal with
the help of high quality factor optical resonance (whispering gallery
mode) of a LSDM. From the theoretical simulations, it has been
found that at least an order of magnitude enhancement in the SERS
signal could be achieved easily using the proposed technique. We
strongly believe that this report will enable the research community
for improving the Raman scattering signals.
Abstract: We investigate relaxation dynamics of a quantum
dipole emitter (QDE), e.g., a molecule or quantum dot, located near a
metal nanoparticle (MNP) exhibiting a dipolar localized surface
plasmon (LSP) resonance at the frequency of the QDE radiative
transition. It is shown that under the condition of the QDE-MNP
characteristic relaxation time being much shorter than that of the
QDE in free-space but much longer than the LSP lifetime. It is also
shown that energy dissipation in the QDE-MNP system is relatively
weak with the probability of the photon emission being about 0.75, a
number which, rather surprisingly, does not explicitly depend on the
metal absorption characteristics. The degree of entanglement
measured by the concurrency takes the maximum value, while the
distances between the QDEs and metal ball approximately are equal.
Abstract: Localized surface plasmon resonance (LSPR) is the
coherent oscillation of conductive electrons confined in noble
metallic nanoparticles excited by electromagnetic radiation, and
nanosphere lithography (NSL) is one of the cost-effective methods to
fabricate metal nanostructures for LSPR. NSL can be categorized
into two major groups: dispersed NSL and closely pack NSL. In
recent years, gold nanocrescents and gold nanoholes with vertical
sidewalls fabricated by dispersed NSL, and silver nanotriangles and
gold nanocaps on silica nanospheres fabricated by closely pack NSL,
have been reported for LSPR biosensing. This paper introduces
several novel gold nanostructures fabricated by NSL in LSPR
applications, including 3D nanostructures obtained by evaporating
gold obliquely on dispersed nanospheres, nanoholes with slant
sidewalls, and patchy nanoparticles on closely packed nanospheres,
all of which render satisfactory sensitivity for LSPR sensing. Since
the LSPR spectrum is very sensitive to the shape of the metal
nanostructures, formulas are derived and software is developed for
calculating the profiles of the obtainable metal nanostructures by
NSL, for different nanosphere masks with different fabrication
conditions. The simulated profiles coincide well with the profiles of
the fabricated gold nanostructures observed under scanning electron
microscope (SEM) and atomic force microscope (AFM), which
proves that the software is a useful tool for the process design of
different LSPR nanostructures.