Abstract: In this study, we illustrated the performance and
microbial community of single- and two-phase systems anaerobically
co-digesting cassava pulp and pig manure. The results showed that
the volatile solid reduction and biogas productivity of two-phase
CSTR were 66 ± 4% and 2000 ± 210 ml l-1 d-1, while those of singlephase
CSTR were 59 ± 1% and 1670 ± 60 ml l-1 d-1, respectively. Codigestion
in two-phase CSTR gave higher 12% solid degradation and
25% methane production than single-phase CSTR. Phylogenetic
analysis of 16S rDNA clone library revealed that the Bacteroidetes
were the most abundant group, followed by the Clostridia in singlephase
CSTR. In hydrolysis/acidification reactor of two-phase system,
the bacteria within the phylum Firmicutes, especially Clostridium,
Eubacteriaceae and Lactobacillus were the dominant phylogenetic
groups. Among the Archaea, Methanosaeta sp. was the exclusive
predominant in both digesters while the relative abundance of
Methanosaeta sp. and Methanospirillum hungatei differed between
the two systems.
Abstract: The present experimental investigation brings about
a comparative study of lactic acid production by pure strains of
Lactobacilli (1) L. delbreuckii (NCIM2025), (2) L. pentosus (NCIM
2912), (3) Lactobacillus sp.(NCIM 2734, (4) Lactobacillus sp.
(NCIM2084) and coculture of strain-1 and Stain-2 in solid bed of
wheat bran, under the influence of different nitrogen sources such as
baker-s yeast, meat extract and proteose peptone. Among the pure
cultures, strain-3 attained lowest pH value of 3.44, hence highest acid
formation 46.41 g/L, while the coculture attained an overall
maximum value 47.56 g/L lactic acid (pH 3.38) at 15 g/L and 20 g/L
level of baker-s yeast, respectively.
Abstract: Yogurts are prepared by fermenting milk with bacterial cultures consisting of a mixture of Streptococcus ssp. thermophilus and Lactobacillus delbrueckii ssp. bulgaricus. The main aim of this investigation was to develop a majority goat yogurt, with the addition of sheep milk in order to have a final product with good physicochemical quality properties and sensorial attributes. Four types of yogurts were prepared presenting the following proportion of goat and sheep milk respectively: C100 – 100%; C80 – 80%/20%; C60 – 60%/40%; C50 – 50%/50%. The goat milk was from the Serrana Jarmelista breed and the sheep milk from the Serra da Estrela breed. The inclusion of sheep milk improved attractiveness to consumers, and it also improved the nutritional value of the product, mainly the fatty acid and mineral contents. The C50 yogurt was preferred by 28% of the panellists, followed by the C100 with 16% and the commercial cow yogurt was 40% of preferences.
Abstract: The technique of inducing micro ecosystem
restoration is one of aquatic ecology engineering methods used to
retrieve the polluted water. Batch scale study, pilot plant study, and
field study were carried out to observe the eutrophication using the
Inducing Ecology Restorative Symbiosis Agent (IERSA) consisting
mainly degraded products by using lactobacillus, saccharomycete,
and phycomycete. The results obtained from the experiments of the
batch scale and pilot plant study allowed us to development the
parameters for the field study. A pond, 5 m to the outlet of a lake,
with an area of 500 m2 and depth of 0.6-1.2 m containing about 500
tons of water was selected as a model. After the treatment with 10
mg IERSA/L water twice a week for 70 days, the micro restoration
mechanisms consisted of three stages (i.e., restoration, impact
maintenance, and ecology recovery experiment after impact). The
COD, TN, TKN, and chlorophyll a were reduced significantly in the
first week. Although the unexpected heavy rain and contaminate
from sewage system might slow the ecology restoration. However,
the self-cleaning function continued and the chlorophyll a reduced
for 50% in one month. In the 4th week, amoeba, paramecium, rotifer,
and red wriggle worm reappeared, and the number of fish flies
appeared up to1000 fish fries/m3. Those results proved that inducing
restorative mechanism can be applied to improve the eutrophication
and to control the growth of algae in the lakes by gaining the selfcleaning
through inducing and competition of microbes. The
situation for growth of fishes also can reach an excellent result due to
the improvement of water quality.
Abstract: A thirty Rahmani weaned male lambs of average body weight (27.28±1.40 kg) were randomly allotted to three similar groups, ten lambs in each, to study the benefit of commercial feed additives Tonilisat (Saccharomyces cerevisiae) and Roemin W2 (Lactobacillus acidophilus, Lactobacillus thermophilus, Bifidobacterium and Lactose) as growth promoters on lambs performance, digestibility, rumen activity and some blood constituents. The experiment lasted about 107 days. Three experimental groups were allotted as control group: received the basal ration, T1 group: received the basal ration supplemented with Tonilisat as (0.5kg/ ton concentrate feed mixture) and T2 group: received the basal ration supplemented with Roemin W2 (1kg/ ton concentrate feed mixture). Our study revealed that addition of Tonilisat significantly increased digestion coefficient of crude protein than that of the control group, Furthermore, the supplementation of Tonilisat or Roemin W2 increased (p
Abstract: Three batches of yogurts were made with soy protein
isolate (SPI) supplemented with 2% (S2), 4% (S4) or 6% (S6) of
skim milk powder (SMP). The fourth batch (control; S0) was
prepared from SPI without SMP supplementation. Lactobacillus
delbrueckii ssp. bulgaricus ATCC 11842 (Lb 11842) and
Streptococcus thermophilus ST 1342 (ST 1342) were used as the
starter culture. Biotransformation of the inactive forms, isoflavone
glycosides (IG) to biologically active forms, isoflavone aglycones
(IA), was determined during 28 d storage. The viability of both
microorganisms was significantly higher (P < 0.05) in S2, S4, and S6
than that in S0. The ratio of lactic acid/acetic acid in S0 was in the
range of 15.53 – 22.31 compared to 7.24 – 12.81 in S2, S4 and S6.
The biotransformation of IG to IA in S2, S4 and S6 was also
enhanced by 9.9 -13.3% compared to S0.