Abstract: The objective of current issue was to develop a model
of testicular herpes simplex virus (HSV) type I infection for
assessment of viral effect on fertility. 56 male mice were inoculated
intraperitoneally with different concentrations of HSV on 8 day post
partum. It was revealed that the optimal dose was 100 plaque
forming units per mice as it provided testicular infection in 100% of
survivors. HSV proteins were detected both in somatic and germ
cells (spermatogonia, spermatocytes, spermatides). Although DNA
load in testis was descending from 3 to 28 days post infection only
12.5% of infected males had offspring after mating with uninfected
females comparing to 87.5% in control (p=0.012). These results are
the first direct evidence for HSV impact in male sterility. Prepuberal
mice appeared to be a suitable model for investigation of
pathogenesis of virus-associated fertility disorders.