Abstract: For identifying the discriminative sequence features between exons and introns, a new paradigm, rescaled-range frameshift analysis (RRFA), was proposed. By RRFA, two new
sequence features, the frameshift sensitivity (FS) and the accumulative
penta-mer complexity (APC), were discovered which
were further integrated into a new feature of larger scale, the persistency in anti-mutation (PAM). The feature-validation experiments
were performed on six model organisms to test the power
of discrimination. All the experimental results highly support that FS, APC and PAM were all distinguishing features between exons
and introns. These identified new sequence features provide new insights into the sequence composition of genes and they have
great potentials of forming a new basis for recognizing the exonintron boundaries in gene sequences.