Abstract: A new strategy for oriented immobilization of proteins was proposed. The strategy contains two steps. The first step is to search for a docking site away from the active site on the protein surface. The second step is trying to find a ligand that is able to grasp the targeted site of the protein. To avoid ligand binding to the active site of protein, the targeted docking site is selected to own opposite charges to those near the active site. To enhance the ligand-protein binding, both hydrophobic and electrostatic interactions need to be included. The targeted docking site should therefore contain hydrophobic amino acids. The ligand is then selected through the help of molecular docking simulations. The enzyme α-amylase derived from Aspergillus oryzae (TAKA) was taken as an example for oriented immobilization. The active site of TAKA is surrounded by negatively charged amino acids. All the possible hydrophobic sites on the surface of TAKA were evaluated by the free energy estimation through benzene docking. A hydrophobic site on the opposite side of TAKA-s active site was found to be positive in net charges. A possible ligand, 3,3-,4,4- – Biphenyltetra- carboxylic acid (BPTA), was found to catch TAKA by the designated docking site. Then, the BPTA molecules were grafted onto silica gels and measured the affinity of TAKA adsorption and the specific activity of thereby immobilized enzymes. It was found that TAKA had a dissociation constant as low as 7.0×10-6 M toward the ligand BPTA on silica gel. The increase in ionic strength has little effect on the adsorption of TAKA, which indicated the existence of hydrophobic interaction between ligands and proteins. The specific activity of the immobilized TAKA was compared with the randomly adsorbed TAKA on primary amine containing silica gel. It was found that the orderly immobilized TAKA owns a specific activity twice as high as the one randomly adsorbed by ionic interaction.
Abstract: The purpose of this paper is to propose a framework for constructing correct parallel processing programs based on Equivalent Transformation Framework (ETF). ETF regards computation as In the framework, a problem-s domain knowledge and a query are described in definite clauses, and computation is regarded as transformation of the definite clauses. Its meaning is defined by a model of the set of definite clauses, and the transformation rules generated must preserve meaning. We have proposed a parallel processing method based on “specialization", a part of operation in the transformations, which resembles substitution in logic programming. The method requires “Memo-tree", a history of specialization to maintain correctness. In this paper we proposes the new method for the specialization-base parallel processing without Memo-tree.
Abstract: Recently the usefulness of Concept Abduction, a novel non-monotonic inference service for Description Logics (DLs), has been argued in the context of ontology-based applications such as semantic matchmaking and resource retrieval. Based on tableau calculus, a method has been proposed to realize this reasoning task in ALN, a description logic that supports simple cardinality restrictions as well as other basic constructors. However, in many ontology-based systems, the representation of ontology would require expressive formalisms for capturing domain-specific constraints, this language is not sufficient. In order to increase the applicability of the abductive reasoning method in such contexts, we would like to present in the scope of this paper an extension of the tableaux-based algorithm for dealing with concepts represented inALCQ, the description logic that extends ALN with full concept negation and quantified number restrictions.
Abstract: For the electrical metrics that describe photovoltaic
cell performance are inherently multivariate in nature, use of a
univariate, or one variable, statistical process control chart can have
important limitations. Development of a comprehensive process
control strategy is known to be significantly beneficial to reducing
process variability that ultimately drives up the manufacturing cost
photovoltaic cells. The multivariate moving average or MMA chart,
is applied to the electrical metrics of photovoltaic cells to illustrate
the improved sensitivity on process variability this method of control
charting offers. The result show the ability of the MMA chart to
expand to as any variables as needed, suggests an application
with multiple photovoltaic electrical metrics being used in
concert to determine the processes state of control.
Abstract: We consider a typical problem in the assembly of
printed circuit boards (PCBs) in a two-machine flow shop system to
simultaneously minimize the weighted sum of weighted tardiness and
weighted flow time. The investigated problem is a group scheduling
problem in which PCBs are assembled in groups and the interest is to
find the best sequence of groups as well as the boards within each
group to minimize the objective function value. The type of setup
operation between any two board groups is characterized as carryover
sequence-dependent setup time, which exactly matches with the real
application of this problem. As a technical constraint, all of the
boards must be kitted before the assembly operation starts (kitting
operation) and by kitting staff. The main idea developed in this paper
is to completely eliminate the role of kitting staff by assigning the
task of kitting to the machine operator during the time he is idle
which is referred to as integration of internal (machine) and external
(kitting) setup times. Performing the kitting operation, which is a
preparation process of the next set of boards while the other boards
are currently being assembled, results in the boards to continuously
enter the system or have dynamic arrival times. Consequently, a
dynamic PCB assembly system is introduced for the first time in the
assembly of PCBs, which also has characteristics similar to that of
just-in-time manufacturing. The problem investigated is
computationally very complex, meaning that finding the optimal
solutions especially when the problem size gets larger is impossible.
Thus, a heuristic based on Genetic Algorithm (GA) is employed. An
example problem on the application of the GA developed is
demonstrated and also numerical results of applying the GA on
solving several instances are provided.
Abstract: Software maintenance and mainly software
comprehension pose the largest costs in the software lifecycle. In
order to assess the cost of software comprehension, various
complexity measures have been proposed in the literature. This paper
proposes new cognitive-spatial complexity measures, which combine
the impact of spatial as well as architectural aspect of the software to
compute the software complexity. The spatial aspect of the software
complexity is taken into account using the lexical distances (in
number of lines of code) between different program elements and the
architectural aspect of the software complexity is taken into
consideration using the cognitive weights of control structures
present in control flow of the program. The proposed measures are
evaluated using standard axiomatic frameworks and then, the
proposed measures are compared with the corresponding existing
cognitive complexity measures as well as the spatial complexity
measures for object-oriented software. This study establishes that the
proposed measures are better indicators of the cognitive effort
required for software comprehension than the other existing
complexity measures for object-oriented software.
Abstract: These days wireless local area networks has become
very popular, when the initial IEEE802.11 is the standard for
providing wireless connectivity to automatic machinery, equipment
and stations that require rapid deployment, which may be portable,
handheld or which may be mounted on moving vehicles within a
local area. IEEE802.11 Wireless local area network is a sharedmedium
communication network that transmits information over
wireless links for all IEEE802.11 stations in its transmission range to
receive. When a user is moving from one location to another, how
the other user knows about the required station inside WLAN. For
that we designed and implemented a system to locate a mobile user
inside the wireless local area network based on RSSI with the help of
four specially designed architectures. These architectures are based
on statistical or we can say manual configuration of mapping and
radio map of indoor and outdoor location with the help of available
Sniffer based and cluster based techniques. We found a better
location of a mobile user in WLAN. We tested this work in indoor
and outdoor environments with different locations with the help of
Pamvotis, a simulator for WLAN.
Abstract: In this research, an anaerobic co-digestion using decanter cake from palm oil mill industry to improve the biogas production from frozen seafood wastewater is studied using Continuously Stirred Tank Reactor (CSTR) process. The experiments were conducted in laboratory-scale. The suitable Hydraulic Retention Time (HRT) was observed in CSTR experiments with 24 hours of mixing time using the mechanical mixer. The HRT of CSTR process impacts on the efficiency of biogas production. The best performance for biogas production using CSTR process was the anaerobic codigestion for 20 days of HRT with the maximum methane production rate of 1.86 l/d and the average maximum methane production of 64.6%. The result can be concluded that the decanter cake can improve biogas productivity of frozen seafood wastewater.
Abstract: Global Positioning System (GPS) technology is widely used today in the areas of geodesy and topography as well as in aeronautics mainly for military purposes. Due to the military usage of GPS, full access and use of this technology is being denied to the civilian user who must then work with a less accurate version. In this paper we focus on the estimation of the receiver coordinates ( X, Y, Z ) and its clock bias ( δtr ) of a fixed point based on pseudorange measurements of a single GPS receiver. Utilizing the instantaneous coordinates of just 4 satellites and their clock offsets, by taking into account the atmospheric delays, we are able to derive a set of pseudorange equations. The estimation of the four unknowns ( X, Y, Z , δtr ) is achieved by introducing an extended Kalman filter that processes, off-line, all the data collected from the receiver. Higher performance of position accuracy is attained by appropriate tuning of the filter noise parameters and by including other forms of biases.
Abstract: Dexamethasone (Dex) is a synthetic glucocorticoid
that is used in therapy. However prolonged treatments with high
doses are often required. This causes side effects that interfere with
the activity of several endocrine systems, including the gonadotropic
axis.
The aim of our study is to determine the effect of Dex on testicular
function in prepubertal Wistar rats.
Newborn Wistar rats are submitted to intraperitoneal injection of
Dex (1μg of Dex dissolved in NaCl 0.9% / 5g bw) for 20 days and
then sacrificed at the age of 40days. A control group received NaCl
0.9%. The rat is weighed daily. The plasmatic levels of testosterone,
LH and FSH were measured by radioimmunoassay. A histomorphometric
study was performed on sections of testis.
Treated groups showed a significant decrease in body weight (p
Abstract: Understanding the number of people and the flow of
the persons is useful for efficient promotion of the institution
managements and company-s sales improvements. This paper
introduces an automated method for counting passerby using virtualvertical
measurement lines. The process of recognizing a passerby is
carried out using an image sequence obtained from the USB camera.
Space-time image is representing the human regions which are
treated using the segmentation process. To handle the problem of
mismatching, different color space are used to perform the template
matching which chose automatically the best matching to determine
passerby direction and speed. A relation between passerby speed and
the human-pixel area is used to distinguish one or two passersby. In
the experiment, the camera is fixed at the entrance door of the hall in
a side viewing position. Finally, experimental results verify the
effectiveness of the presented method by correctly detecting and
successfully counting them in order to direction with accuracy of
97%.
Abstract: Hand gesture is one of the typical methods used in
sign language for non-verbal communication. It is most commonly
used by people who have hearing or speech problems to
communicate among themselves or with normal people. Various sign
language systems have been developed by manufacturers around the
globe but they are neither flexible nor cost-effective for the end
users. This paper presents a system prototype that is able to
automatically recognize sign language to help normal people to
communicate more effectively with the hearing or speech impaired
people. The Sign to Voice system prototype, S2V, was developed
using Feed Forward Neural Network for two-sequence signs
detection. Different sets of universal hand gestures were captured
from video camera and utilized to train the neural network for
classification purpose. The experimental results have shown that
neural network has achieved satisfactory result for sign-to-voice
translation.
Abstract: The aim of this study was to estimate the frequency of
EBV infection in Hodgkin's lymphoma (HL) and non-Hodgkin's
lymphoma (NHL) occurring in Jordanian patients. A total of 55
patients with lymphoma were examined in this study. Of 55 patients,
30 and 25 were diagnosed as HL and NHL, respectively. The four
HL subtypes were observed with the majority of the cases exhibited
the mixed cellularity (MC) subtype followed by the nodular sclerosis
(NS). The high grade was found to be the commonest subtype of
NHL in our sample, followed by the low grade. The presence of EBV
virus was detected by immunostating for expression of latent
membrane protein-1 (LMP-1). The frequency of LMP-1 expression
occurred more frequent in patients with HL (60.0%) than in patients
with NHL (32.0%). The frequency of LMP-1 expression was also
higher in patients with MC subtype (61.11%) than those patients with
NS (28.57%). No age or gender difference in occurrence of EBV
infection was observed among patient with HL. By contrast, the
prevalence of EBV infection in NHL patients aged below 50 was
lower (16.66%) than in NHL patients aged 50 or above (46.15%). In
addition, EBV infection was more frequent in females with NHL
(38.46%) than in male with NHL (25%). In NHL cases, the
frequency of EBV infection in intermediate grade (60.0%) was high
when compared with frequency of low (25%) or high grades (25%).
In conclusion, analysis of LMP-1 expression indicates an important
role for this viral oncogene in the pathogenesis of EBV-associated
malignant lymphomas. These data also support the previous findings
that people with EBV may develop lymphoma and that efforts to
maintain low lymphoma should be considered for people with EBV
infection.
Abstract: The use of the mechanical simulation (in particular the finite element analysis) requires the management of assumptions in order to analyse a real complex system. In finite element analysis (FEA), two modeling steps require assumptions to be able to carry out the computations and to obtain some results: the building of the physical model and the building of the simulation model. The simplification assumptions made on the analysed system in these two steps can generate two kinds of errors: the physical modeling errors (mathematical model, domain simplifications, materials properties, boundary conditions and loads) and the mesh discretization errors. This paper proposes a mesh adaptive method based on the use of an h-adaptive scheme in combination with an error estimator in order to choose the mesh of the simulation model. This method allows us to choose the mesh of the simulation model in order to control the cost and the quality of the finite element analysis.
Abstract: In the present study, development of salbutamol
sulphate nanoparticles that adhere to gastric mucus was investigated.
Salbutamol sulphate has low bioavailability due to short transit time in
gastric. It also has a positive surface charge that provides hurdles to be
encapsulated by the positively strong mucoadhesive polymer of
chitosan. To overcome the difficulties, the surface charge of active
ingredient was modified using several nonionic and anionic
stomach-specific polymers. The nanoparticles were prepared using
ionotropic gelation technique. The evaluation involved determination
of particle size, zeta potential, entrapment efficiency, in vitro drug
release and in vitro mucoadhesion test. Results exhibited that the use
of anionic alginate polymer was more satisfactory than that of
nonionic polymer. Characteristics of the particles was nano-size, high
encapsulation efficiency, fulfilled the drug release requirements and
adhesive towards stomach for around 11 hours. This result shows that
the salbutamol sulphate nanoparticles can be utilized for improvement
its delivery.
Abstract: The Chichiawan stream in the Wulin catchment in
Taiwan is the natural habitat of Formosan landlocked salmon. Human
and agriculture activities gradually worsen water quality and impact
the fish habitat negatively. To protect and manage Formosan
landlocked salmon habitat, it is important to understand a variety
land-uses affect on the watershed responses to storms. This study
discusses watershed responses to the dry-day before a storm event and
a variety of land-uses in the Wulin catchment. Under the land-use
planning in the Wulin catchment, the peak flows during typhoon
events do not have noticeable difference. However, the nutrient
exports can be highly reduced under the strategies of restraining
agriculture activities. Due to the higher affinity of P for soil than that
of N, the exports of TN from overall Wuling catchment were much
greater than Ortho-P. Agriculture mainly centralized in subbasin A,
which is the important source of nutrients in nonpoint source discharge.
The subbasin A supplied about 26% of the TN and 32% of the Ortho-P
discharge in 2004, despite the fact it only covers 19% area of the
Wuling catchment. The subbasin analysis displayed that the
agricultural subbasin A exports higher nutrients per unit area than
other forest subbasins. Additionally, the agricultural subbasin A
contributed a higher percentage to total Ortho-P exports compares to
TN. The results of subbasin analysis might imply the transport of
Ortho-P was similar to the particulate matter which was mainly
influenced by the runoff and affected by the desorption from soil
particles while the TN (dominated as nitrate-N) was mainly influenced
by base-flow.
Abstract: Sleep stage scoring is the process of classifying the
stage of the sleep in which the subject is in. Sleep is classified into
two states based on the constellation of physiological parameters.
The two states are the non-rapid eye movement (NREM) and the
rapid eye movement (REM). The NREM sleep is also classified into
four stages (1-4). These states and the state wakefulness are
distinguished from each other based on the brain activity. In this
work, a classification method for automated sleep stage scoring
based on a single EEG recording using wavelet packet decomposition
was implemented. Thirty two ploysomnographic recording from the
MIT-BIH database were used for training and validation of the
proposed method. A single EEG recording was extracted and
smoothed using Savitzky-Golay filter. Wavelet packets
decomposition up to the fourth level based on 20th order Daubechies
filter was used to extract features from the EEG signal. A features
vector of 54 features was formed. It was reduced to a size of 25 using
the gain ratio method and fed into a classifier of regression trees. The
regression trees were trained using 67% of the records available. The
records for training were selected based on cross validation of the
records. The remaining of the records was used for testing the
classifier. The overall correct rate of the proposed method was found
to be around 75%, which is acceptable compared to the techniques in
the literature.
Abstract: The next stage of the home networking environment is
supposed to be ubiquitous, where each piece of material is equipped
with an RFID (Radio Frequency Identification) tag. To fully support
the ubiquitous environment, home networking middleware should be
able to recommend home services based on a user-s interests and
efficiently manage information on service usage profiles for the users.
Therefore, USN (Ubiquitous Sensor Network) technology, which
recognizes and manages a appliance-s state-information (location,
capabilities, and so on) by connecting RFID tags is considered. The
Intelligent Multi-Agent Middleware (IMAM) architecture was
proposed to intelligently manage the mobile RFID-based home
networking and to automatically supply information about home
services that match a user-s interests. Evaluation results for
personalization services for IMAM using Bayesian-Net and Decision
Trees are presented.
Abstract: The purpose of this research is to develop and apply the
RSCMAC to enhance the dynamic accuracy of Global Positioning
System (GPS). GPS devices provide services of accurate positioning,
speed detection and highly precise time standard for over 98% area on
the earth. The overall operation of Global Positioning System includes
24 GPS satellites in space; signal transmission that includes 2
frequency carrier waves (Link 1 and Link 2) and 2 sets random
telegraphic codes (C/A code and P code), on-earth monitoring stations
or client GPS receivers. Only 4 satellites utilization, the client position
and its elevation can be detected rapidly. The more receivable
satellites, the more accurate position can be decoded. Currently, the
standard positioning accuracy of the simplified GPS receiver is greatly
increased, but due to affected by the error of satellite clock, the
troposphere delay and the ionosphere delay, current measurement
accuracy is in the level of 5~15m. In increasing the dynamic GPS
positioning accuracy, most researchers mainly use inertial navigation
system (INS) and installation of other sensors or maps for the
assistance. This research utilizes the RSCMAC advantages of fast
learning, learning convergence assurance, solving capability of
time-related dynamic system problems with the static positioning
calibration structure to improve and increase the GPS dynamic
accuracy. The increasing of GPS dynamic positioning accuracy can be
achieved by using RSCMAC system with GPS receivers collecting
dynamic error data for the error prediction and follows by using the
predicted error to correct the GPS dynamic positioning data. The
ultimate purpose of this research is to improve the dynamic positioning
error of cheap GPS receivers and the economic benefits will be
enhanced while the accuracy is increased.
Abstract: The heavy metal contamination of the technogenous
sediments and soils at the investigated dump-field show irregular
planar distribution. Also the heavy metal content in the surface water,
drainage water and in the groundwater was studied both in the dry as
well as during the rainy periods. The cementation process causes
substitution of iron by copper. Natural installation and development
of plant species was observed at the old mine waste dumps, specific
to the local chemical conditions such as low content of essential
nutrients and high content of heavy metals. The individual parts of
the plant tissues (roots, branches/stems, leaves/needles, flowers/
fruits) are contaminated by heavy metals and tissues are damaged
differently, respectively.