Abstract: The dilute acid pretreatment and enzymatic
saccharification of lignocellulosic substrate, cogon grass (Imperata
cylindrical, L.) was optimized prior ethanol fermentation using
simultaneous saccharification and fermentation (SSF) method. The
optimum pretreatment conditions, temperature, sulfuric acid
concentration, and reaction time were evaluated by determining the
maximum sugar yield at constant enzyme loading. Cogon grass, at
10% w/v substrate loading, has optimum pretreatment conditions of
126°C, 0.6% v/v H2SO4, and 20min reaction time. These
pretreatment conditions were used to optimize enzymatic
saccharification using different enzyme combinations. The maximum
saccharification yield of 36.68mg/mL (71.29% reducing sugar) was
obtained using 25FPU/g-cellulose cellulase complex combined with
1.1% w/w of cellobiase, ß-glucosidase, and 0.225% w/w of
hemicellulase complex, after 96 hours of saccharification. Using the
optimum pretreatment and saccharification conditions, SSF of treated
substrates was done at 37°C for 120 hours using industrial yeast
strain HBY3, Saccharomyces cerevisiae. The ethanol yield for cogon
grass at 4% w/w loading was 9.11g/L with 5.74mg/mL total residual
sugar.
Abstract: Optimization study of the diesters biolubricant oleyl 9(12)-hydroxy-10(13)-oleioxy-12(9)-octadecanoate (OLHYOOT) was synthesized in the presence of sulfuric acid (SA) as catalyst has been done. Optimum conditions of the experiment to obtain high yield% of OLHYOOT were predicted at ratio of OL/HYOOA of 1:1 g/g, ratio of SA/HYOOA of 0.20:1 g/g, reaction temperature 110 °C and 4.5 h of reaction time. At this condition, the Yield% of OLHYOOT was 88.7. Disappearance of carboxylic acid (C=O) peak has observed by FTIR with appearance ester (C=O) at 1738 cm-1. 1H NMR spectra analyses confirmed the result of OLHYOOT with appearance ester (-CHOCOR) at 4.05ppm and also the 13C-NMR confirmed the result with appearance ester (C=O) peak at 173.93ppm.