Optimization of Pretreatment and Enzymatic Saccharification of Cogon Grass Prior Ethanol Production
The dilute acid pretreatment and enzymatic
saccharification of lignocellulosic substrate, cogon grass (Imperata
cylindrical, L.) was optimized prior ethanol fermentation using
simultaneous saccharification and fermentation (SSF) method. The
optimum pretreatment conditions, temperature, sulfuric acid
concentration, and reaction time were evaluated by determining the
maximum sugar yield at constant enzyme loading. Cogon grass, at
10% w/v substrate loading, has optimum pretreatment conditions of
126°C, 0.6% v/v H2SO4, and 20min reaction time. These
pretreatment conditions were used to optimize enzymatic
saccharification using different enzyme combinations. The maximum
saccharification yield of 36.68mg/mL (71.29% reducing sugar) was
obtained using 25FPU/g-cellulose cellulase complex combined with
1.1% w/w of cellobiase, ß-glucosidase, and 0.225% w/w of
hemicellulase complex, after 96 hours of saccharification. Using the
optimum pretreatment and saccharification conditions, SSF of treated
substrates was done at 37°C for 120 hours using industrial yeast
strain HBY3, Saccharomyces cerevisiae. The ethanol yield for cogon
grass at 4% w/w loading was 9.11g/L with 5.74mg/mL total residual
sugar.
[1] A. Wingren, M. Galbe, and G. Zacchi, "Techno
producing ethanol from softwood: comparison of SSF and SHF and
identification of bottlenecks,"
pp. 1109-1117.
[2] S. Kim, and B.E. Dale, "Global potential bioethanol production from
wasted crops and crop residues,"
pp. 361-375.
[3] Y. Sun, J.Y. Cheng, "Hydrolysis of lignocellulosic materials for ethanol
production: a review," Bioresource Technology
[4] R. M. Brook, "Review of literature on
Raeuschel with particular reference to South East Asia,"
Management, vol. 35, 1989, pp. 12
[5] S. K. McDonald, D. G. Shilling, C. A. N. Okoli, T. A. Bewick, D.
Gordon, D. Hall, and R. Smith, "Population dynamics of cogongrass,
Imperata cylindrical," Proceedings of the Southern Weed
Society, vol. 49, 1996, p. 156.
[6] N. Mosier, C. Wyman, B. Dale, R. Elander, Y. Y. Lee, M. Holtzapple
and M. Ladisch, "Features of p
lignocellulosic biomass," Bioresource Technology
673-686.
[7] B. Zhnag, L. Wang, A. Shahbazi, O. Diallo and A. Whitmore, "Dilute
sulphuric acid pretreatment of cattails for cellulose conversion,"
Bioresource Technology, vol. 19, 2011, pp. 9308
[8] D. Schell, J. Farmer, M. Newman and J. McMillan, "Dilute sulfuric acid
pretreatment of corn stover in pilot
and Biotechnology, vol. 105, 2003, pp. 69
Techno-economic evaluation of
Biotechnology Progress, vol. 19, 2003,
obal Biomass and Bioenergy, vol. 26, 2004,
Technology, vol. 83, 2002, pp. 1-11.
Imperata cylindrica (L.)
Tropical Pest
, 12-25.
on, Science
promising technologies for pretreatment of
Technology, vol. 96, 2005, pp.
, 9308-9312.
pilot-scale reactor," Applied Biochemistry
, 69-85.
[1] A. Wingren, M. Galbe, and G. Zacchi, "Techno
producing ethanol from softwood: comparison of SSF and SHF and
identification of bottlenecks,"
pp. 1109-1117.
[2] S. Kim, and B.E. Dale, "Global potential bioethanol production from
wasted crops and crop residues,"
pp. 361-375.
[3] Y. Sun, J.Y. Cheng, "Hydrolysis of lignocellulosic materials for ethanol
production: a review," Bioresource Technology
[4] R. M. Brook, "Review of literature on
Raeuschel with particular reference to South East Asia,"
Management, vol. 35, 1989, pp. 12
[5] S. K. McDonald, D. G. Shilling, C. A. N. Okoli, T. A. Bewick, D.
Gordon, D. Hall, and R. Smith, "Population dynamics of cogongrass,
Imperata cylindrical," Proceedings of the Southern Weed
Society, vol. 49, 1996, p. 156.
[6] N. Mosier, C. Wyman, B. Dale, R. Elander, Y. Y. Lee, M. Holtzapple
and M. Ladisch, "Features of p
lignocellulosic biomass," Bioresource Technology
673-686.
[7] B. Zhnag, L. Wang, A. Shahbazi, O. Diallo and A. Whitmore, "Dilute
sulphuric acid pretreatment of cattails for cellulose conversion,"
Bioresource Technology, vol. 19, 2011, pp. 9308
[8] D. Schell, J. Farmer, M. Newman and J. McMillan, "Dilute sulfuric acid
pretreatment of corn stover in pilot
and Biotechnology, vol. 105, 2003, pp. 69
Techno-economic evaluation of
Biotechnology Progress, vol. 19, 2003,
obal Biomass and Bioenergy, vol. 26, 2004,
Technology, vol. 83, 2002, pp. 1-11.
Imperata cylindrica (L.)
Tropical Pest
, 12-25.
on, Science
promising technologies for pretreatment of
Technology, vol. 96, 2005, pp.
, 9308-9312.
pilot-scale reactor," Applied Biochemistry
, 69-85.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:50551", author = "Jhalique Jane R. Fojas and Ernesto J. Del Rosario", title = "Optimization of Pretreatment and Enzymatic Saccharification of Cogon Grass Prior Ethanol Production", abstract = "The dilute acid pretreatment and enzymatic
saccharification of lignocellulosic substrate, cogon grass (Imperata
cylindrical, L.) was optimized prior ethanol fermentation using
simultaneous saccharification and fermentation (SSF) method. The
optimum pretreatment conditions, temperature, sulfuric acid
concentration, and reaction time were evaluated by determining the
maximum sugar yield at constant enzyme loading. Cogon grass, at
10% w/v substrate loading, has optimum pretreatment conditions of
126°C, 0.6% v/v H2SO4, and 20min reaction time. These
pretreatment conditions were used to optimize enzymatic
saccharification using different enzyme combinations. The maximum
saccharification yield of 36.68mg/mL (71.29% reducing sugar) was
obtained using 25FPU/g-cellulose cellulase complex combined with
1.1% w/w of cellobiase, ß-glucosidase, and 0.225% w/w of
hemicellulase complex, after 96 hours of saccharification. Using the
optimum pretreatment and saccharification conditions, SSF of treated
substrates was done at 37°C for 120 hours using industrial yeast
strain HBY3, Saccharomyces cerevisiae. The ethanol yield for cogon
grass at 4% w/w loading was 9.11g/L with 5.74mg/mL total residual
sugar.", keywords = "Acid pretreatment, bioethanol, biomass, cogon grass,
fermentation, lignocellylose, SSF.", volume = "7", number = "5", pages = "251-4", }