Abstract: Heavy metals have bad effects on environment and
soils and it can uptake by natural HAP .natural Hap is an inexpensive
material that uptake large amounts of various heavy metals like Zn
(II) .Natural HAP (N-HAP), extracted from bovine cortical bone ash,
is a good choice for substitution of commercial HAP. Several
experiments were done to investigate the sorption capacity of Zn (II)
to N-HAP in various particles sizes, temperatures, initial
concentrations, pH and reaction times. In this study, the sorption of
Zinc ions from a Zn solution onto HAP particles with sizes of 1537.6
nm and 47.6 nm at three initial pH values of 4.50, 6.00 and 7.50 was
studied. The results showed that better performance was obtained
through a 47.6 nm particle size and higher pH values. The
experimental data were analyzed using Langmuir, Freundlich, and
Arrhenius equations for equilibrium, kinetic and thermodynamic
studies. The analysis showed a maximum adsorption capacity of NHAP
as being 1.562 mmol/g at a pH of 7.5 and small particle size.
Kinetically, the prepared N-HAP is a feasible sorbent that retains Zn
(II) ions through a favorable and spontaneous sorption process.
Abstract: The effects of commercial or bovine yeasts on the
performance and blood variables of broiler chickens intoxicated with
aflatoxin were investigated in broilers. Four hundred eighty broilers
(Arbor Acres; 3-wk-old) were randomly assigned to 4 groups. Each
group (120 broiler chickens) was further randomly divided into 6
replicates of 20 chickens. The treatments were control diet without
additives (treatment 1), 250 ppb AFB1 (treatment 2), commercial
yeast, Saccharomyces cerevisiae, (CY 2.5 x 107 CFU/g) + 250 ppb
AFB1 (treatment 3) and bovine yeast, Saccharomyces cerevisiae,
(BY 2.5 x 107 CFU/g + 250 ppb AFB1 (treatment 4). Complete
randomized design (CRD) was used in the experiment. Feed
consumption and body weight were recorded at every five-day
period. On day 42, carcass compositions were determined from 30
birds per treatment. While chicks were sacrificed, 3-4 ml blood
sample was taken and stored frozen at (-20°C) for serum chemical
analysis to determine effects of consumption of diets on blood
chemistry (total protein, albumin, glucose, urea, cholesterol and
triglycerides). There were no significant differences in ADFI among
the treatments(P>0.05). However, BWG, FCR and mortality were
highly significantly different (P
Abstract: This is a genetic comparison study of Arabian Oryx
(Oryx leucoryx) population at two different locations (A &B) based
on nuclear microsatellite DNA markers. Arabian Oryx is listed as
vulnerable and endanger by the World Conservation Union (IUCN).
Thirty microsatellite markers from bovine family were applied to
investigate the genetic diversity of the Arabian Oryx and to set up a
molecular inventory. Among 30 microsatellite markers used, 13
markers were moderately polymorphic. Arabian Oryx at location A
has shown better gene diversity over location B. However, mean
number of alleles were less than location B. Data of within
population inbreeding coefficient indicates inbreeding at both
locations (A&B). Based on the analysis of polymorphic microsatellite
markers, the study revealed that Arabian Oryx need a genetically
designed breeding program.
Abstract: Aim of this work was to compare the efficacy of two
loading methods of proteins onto polymeric nanocarriers: adsorption
and encapsulation methods. Preliminary studies of protein loading
were done using Bovine Serum Albumin (BSA) as model protein.
Nanocarriers were prepared starting from polylactic co-glycolic acid
(PLGA) polymer; production methods used are two different variants
of emulsion evaporation method. Nanoparticles obtained were
analyzed in terms of dimensions by Dynamic Light Scattering and
Loading Efficiency of BSA by Bradford Assay. Loaded
nanoparticles were then submitted to in-vitro protein dissolution test
in order to study the effect of the delivery system on the release rate
of the protein.
Abstract: Bovine viral diarrhea virus (BVDV) can cause lifelong
persistent infection. One reason for the phenomena is attributed to
BVDV infection to placenta tissue. However the mechanisms that
BVDV invades into placenta tissue remain unclear. To clarify the
molecular mechanisms, we investigated the possible means that
BVDV entered into bovine trophoblast cells (TPC). Yeast two-hybrid
system was used to identify proteins extracted from TPC, which
interact with BVDV envelope glycoprotein E2. A PGbkt7-E2 yeast
expression vector and TPC cDNA library were constructed. Through
two rounds of screening, three positive clones were identified.
Sequencing analysis indicated that all the three positive clones
encoded the same protein clathrin. Physical interaction between
clathrin and BVDV E2 protein was further confirmed by
coimmunoprecipitation experiments. This result suggested that the
clathrin might play a critical role in the process of BVDV entry into
placenta tissue and might be a novel antiviral target for preventing
BVDV infection.
Abstract: In recent years, there has been an increasing interest
toward the use of bovine genotyped embryos for commercial embryo
transfer programs. Biopsy of a few cells in morulla stage is essential
for preimplantation genetic diagnosis (PGD). Low amount of DNA
have limited performing the several molecular analyses within PGD
analyses. Whole genome amplification (WGA) promises to eliminate
this problem. We evaluated the possibility and performance of an
improved primer extension preamplification (I-PEP) method with a
range of starting bovine genomic DNA from 1-8 cells into the WGA
reaction. We optimized a short and simple I-PEP (ssI-PEP) procedure
(~3h). This optimized WGA method was assessed by 6 loci specific
polymerase chain reactions (PCRs), included restriction fragments
length polymorphism (RFLP). Optimized WGA procedure possesses
enough sensitivity for molecular genetic analyses through the few
input cells. This is a new era for generating characterized bovine
embryos in preimplantation stage.
Abstract: Polarization modulation infrared reflection absorption
spectroscopy (PM-IRRAS) in combination with electrochemistry,
was employed to study the influence of surface charge (potential) on
the kinetics of bovine serum albumin (BSA) adsorption on a
biomedical-grade 316LVM stainless steel surface is discussed. The
BSA adsorption kinetics was found to greatly depend on the surface
potential. With an increase in surface potential towards more
negative values, both the BSA initial adsorption rate and the
equilibrium (saturated) surface concentration also increased. Both
effects were explained on the basis of replacement of well-ordered
water molecules at the 316LVM / solution interface, i.e. by the
increase in entropy of the system.
Abstract: The objective of the present study was to evaluate the
potential of hollow microneedles for enhancing the transdermal
delivery of Bovine Serum Albumin (MW~66,000 Da)-Fluorescein
Isothiocyanate (BSA-FITC) conjugate, a hydrophilic large molecular
compound. Moreover, the effect of different formulations was
evaluated. The series of binary mixtures composed of propylene
glycol (PG) and pH 7.4 phosphate buffer solution (PBS) was
prepared and used as a medium for BSA-FITC. The results showed
that there was no permeation of BSA-FITC solution across the
neonatal porcine skin without using hollow microneedles, whereas
the cumulative amount of BSA-FITC released at 8 h through the
neonatal porcine skin was about 60-70% when using hollow
microneedles. Furthermore, the results demonstrated that the higher
volume of PG in binary mixtures injected, the lower cumulative
amount of BSA-FITC released and release rate of BSA-FITC from
skin. These release profiles of BSA-FITC in binary mixtures were
expressed by Fick-s law of diffusion. These results suggest the
utilization of hollow microneedle to enhance transdermal delivery of
protein and provide useful information for designing an effective
hollow microneedle system.
Abstract: Forty-five dairy cows were used to compare the
enzyme activity of alkaline phosphatase (ALP), lactate
dehydrogenase (LDH), α -amylase in the cervical mucus of cows
during spontaneous and induced estrus using progestagen or PGF2 α
and to determine whether these enzymes affect the fertility in cows
with induced estrus, at the time of Al. The animals were assigned to 3
groups (no treatment, a Crestar® for 12 days, a double im injection of
PGF2 α). The cows were artificially inseminated (AI). Cervical
mucus samples were collected from all cows 3 to 5 min before the
AI. The results are summarized as follows: ALP and α -amylase
activity for spontaneous estrus were similar to those for induced
estrus (P>0.05) . LDH activity levels during spontaneous and PGF2 α
induced estrus was significantly lower (P < 0.001) than that in
progestagene induced estrus groups. While no difference was found
between the first and the third groups. Our result showed a significant
difference in LDH activity levels between cows conceived with 2 or
more AI and those conceived with 1 AI. The result of this study
showed that the enzyme activity in cervical mucus is helpful for
detection of ovulation and time of AI.
Abstract: Multi-residue analysis method for penicillins was
developed and validated in bovine muscle, chicken, milk, and flatfish.
Detection was based on liquid chromatography tandem mass
spectrometry (LC/MS/MS). The developed method was validated for
specificity, precision, recovery, and linearity. The analytes were
extracted with 80% acetonitrile and clean-up by a single
reversed-phase solid-phase extraction step. Six penicillins presented
recoveries higher than 76% with the exception of Amoxicillin
(59.7%). Relative standard deviations (RSDs) were not more than
10%. LOQs values ranged from 0.1 and to 4.5 ug/kg. The method was
applied to 128 real samples. Benzylpenicillin was detected in 15
samples and Cloxacillin was detected in 7 samples. Oxacillin was
detected in 2 samples. But the detected levels were under the MRL
levels for penicillins in samples.
Abstract: As part of national epidemiological survey on bovine
viral diarrhea virus (BVDV), a total of 274 dejecta samples were
collected from 14 cattle farms in 8 areas of Xinjiang Uygur
Autonomous Region in northwestern China. Total RNA was extracted
from each sample, and 5--untranslated region (UTR) of BVDV
genome was amplified by using two-step reverse
transcriptase-polymerase chain reaction (RT-PCR). The PCR products
were subsequently sequenced to study the genetic variations of BVDV
in these areas. Among the 274 samples, 33 samples were found
virus-positive. According to sequence analysis of the PCR products,
the 33 samples could be arranged into 16 groups. All the sequences,
however, were highly conserved with BVDV Osloss strains. The virus
possessed theses sequences belonged to BVDV-1b subtype by
phylogenetic analysis. Based on these data, we established a typing
tree for BVDV in these areas. Our results suggested that BVDV-1b
was a predominant subgenotype in northwestern China and no
correlation between the genetic and geographical distances could be
observed above the farm level.
Abstract: The lubricating properties of commercially available
mucins originating from different animal organs, namely bovine
submaxillary mucin (BSM) and porcine gastric mucin (PGM), have
been characterized at polymeric surfaces for biomedical applications.
Atomic force microscopy (AFM) and pin-on-disk tribometry have
been employed for tribological studies at nanoscale and macroscale
contacts, respectively. Polystyrene (PS) was employed to represent
‘rigid’ contacts, whereas poly(dimethylsiloxane) (PDMS) was
employed to represent ‘soft contacts’. To understand the lubricating
properties of mucins in correlation with the coverage on surfaces,
adsorption properties of mucins onto the polymeric substrates have
been characterized by means of optical waveguide light-mode
spectroscopy (OWLS). Both mucins showed facile adsorption onto
both polymeric substrates, but the lubricity was highly dependent
upon the pH change between 2 and 7.