Abstract: Agriculture is the backbone of economy of Pakistan
and cotton is the major agricultural export and supreme source of raw
fiber for our textile industry. To combat severe problems of insect
and weed, combination of three genes namely Cry1Ac, Cry2A and
EPSPS genes was transferred in locally cultivated cotton variety
MNH-786 with the use of Agrobacterium mediated genetic
transformation. The present study focused on the molecular screening
of transgenic cotton plants at T3 generation in order to confirm
integration and expression of all three genes (Cry1Ac, Cry2A and
EPSP synthase) into the cotton genome. Initially, glyphosate spray
assay was used for screening of transgenic cotton plants containing
EPSP synthase gene at T3 generation. Transgenic cotton plants which
were healthy and showed no damage on leaves were selected after 07
days of spray. For molecular analysis of transgenic cotton plants in
the laboratory, the genomic DNA of these transgenic cotton plants
were isolated and subjected to amplification of the three genes. Thus,
seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A
gene) and all twenty (EPSP synthase gene) were produced positive
amplification. On the base of PCR amplification, ten transgenic plant
samples were subjected to protein expression analysis through
ELISA. The results showed that eight out of ten plants were actively
expressing the three transgenes. Real-time PCR was also done to
quantify the mRNA expression levels of Cry1Ac and EPSP synthase
gene. Finally, eight plants were confirmed for the presence and active
expression of all three genes at T3 generation.
Abstract: This study examines analytically the effect of tsunami loads on reinforced concrete (RC) frame buildings. The impact of tsunami wave loads and waterborne objects are analyzed using a typical substandard full-scale two-story RC frame building tested as part of the EU-funded Ecoleader project. The building was subjected to shake table tests in bare condition, and subsequently strengthened using Carbon Fiber Reinforced Polymers (CFRP) composites and retested. Numerical models of the building in both bare and CFRP-strengthened conditions are calibrated in DRAIN-3DX software to match the test results. To investigate the response of wave loads and impact forces, the numerical models are subjected to nonlinear dynamic analyses using force time-history input records. The analytical results are compared in terms of displacements at the floors and at the “impact point” of a boat. The results show that the roof displacement of the CFRP-strengthened building reduced by 63% when compared to the bare building. The results also indicate that strengthening only the mid-height of the impact column using CFRP is more effective at reducing damage when compared to strengthening other parts of the column. Alternative solutions to mitigate damage due to tsunami loads are suggested.
Abstract: This paper examines how “Zakat” provides fair
income redistribution and aids the struggle against poverty. Providing
fair income redistribution and combating poverty constitutes some of
the fundamental tasks performed by countries all over the world.
Each country seeks a solution for these problems according to their
political, economic and administrative styles through applying
various economic and financial policies. The same situation can be
handled via “zakat” association in Islam. Nowadays, we observe
different versions of “zakat” in developed countries. Applications
such as negative income tax denote merely a different form of
“zakat” that is being applied almost in the same way but under
changed names. However, the minimum values to donate under zakat
(e.g. 85 gr. gold and 40 animals) get altered and various amounts are
put into practice. It might be named as negative income tax instead of
zakat, nonetheless, these applications are based on the Holy Koran
and the hadith released 1400 years ago. Besides, considering the
savage and slavery in the world at those times, we might easily
recognize the true value of the zakat being applied for the first time
then in the Islamic system. Through zakat, governments are able to
transfer incomes to the poor as a means of enabling them achieve the
minimum standard of living required. With regards to who benefits
from the Zakat, an objective and fair criteria was used to determine
who benefits from the zakat contrary to the notion that it was based
on peoples’ own choices. Since the zakat is obligatory, the transfers
do not get forwarded directly but via the government and get
distributed, which requires vast governmental organizations. Through
the application of Zakat, reduced levels of poverty can be achieved
and also ensure the fair income redistribution.
Abstract: Estimation of model parameters is necessary to predict
the behavior of a system. Model parameters are estimated using
optimization criteria. Most algorithms use historical data to estimate
model parameters. The known target values (actual) and the output
produced by the model are compared. The differences between the
two form the basis to estimate the parameters. In order to compare
different models developed using the same data different criteria are
used. The data obtained for short scale projects are used here. We
consider software effort estimation problem using radial basis
function network. The accuracy comparison is made using various
existing criteria for one and two predictors. Then, we propose a new
criterion based on linear least squares for evaluation and compared
the results of one and two predictors. We have considered another
data set and evaluated prediction accuracy using the new criterion.
The new criterion is easy to comprehend compared to single statistic.
Although software effort estimation is considered, this method is
applicable for any modeling and prediction.
Abstract: Biofuels production has come forth as a future
technology to combat the problem of depleting fossil fuels. Bio-based
ethanol production from enzymatic lignocellulosic biomass
degradation serves an efficient method and catching the eye of
scientific community. High cost of the enzyme is the major obstacle
in preventing the commercialization of this process. Thus main
objective of the present study was to optimize composition of
medium components for enhancing cellulase production by newly
isolated strain of Bacillus tequilensis. Nineteen factors were taken
into account using statistical Plackett-Burman Design. The significant
variables influencing the cellulose production were further employed
in statistical Response Surface Methodology using Central
Composite Design for maximizing cellulase production. The
optimum medium composition for cellulase production was: peptone
(4.94 g/L), ammonium chloride (4.99 g/L), yeast extract (2.00 g/L),
Tween-20 (0.53 g/L), calcium chloride (0.20 g/L) and cobalt chloride
(0.60 g/L) with pH 7, agitation speed 150 rpm and 72 h incubation at
37oC. Analysis of variance (ANOVA) revealed high coefficient of
determination (R2) of 0.99. Maximum cellulase productivity of 11.5
IU/ml was observed against the model predicted value of 13 IU/ml.
This was found to be optimally active at 60oC and pH 5.5.
Abstract: In this investigation, we have evaluated the effects of
arsenic trioxide on hepatic function in pregnant and lactating Swiss
albino mice and their suckling pups. Experiments were carried out on
female mice given 175 ppm As2O3 in their drinking water from the
14th day of pregnancy until day 14 after delivery. Our results showed
a significant decrease in plasma levels of total protein and albumin,
cholesterol and triglyceride in As2O3 treated mice and their pups. The
hyperbilirubinemia and the increased plasma total alkaline
phosphatase activity suggested the presence of cholestasis.
Transaminase activities as well as lactate deshydrogenase activity in
plasma, known as biomarkers of hepatocellular injury, were elevated
indicating hepatic cells’ damage after treatment with As2O3.
Exposure to arsenic led to an increase of liver thiobarbituric acid
reactive substances level along with a concomitant decrease in the
activities of superoxide dismutase, catalase and glutathione
peroxidase and in glutathione.
Abstract: Graphical User Interface (GUI) is essential to
programming, as is any other characteristic or feature, due to the fact
that GUI components provide the fundamental interaction between
the user and the program. Thus, we must give more interest to GUI
during building and development of systems. Also, we must give a
greater attention to the user who is the basic corner in the dealing
with the GUI. This paper introduces an approach for designing GUI
from one of the models of business workflows which describe the
workflow behavior of a system, specifically through Activity
Diagrams (AD).
Abstract: As the use of geothermal energy grows internationally
more effort is required to monitor and protect areas with rare and
important geothermal surface features. A number of approaches are
presented for developing and calibrating numerical geothermal
reservoir models that are capable of accurately representing
geothermal surface features. The approaches are discussed in the
context of cases studies of the Rotorua geothermal system and the
Orakei-korako geothermal system, both of which contain important
surface features. The results show that models are able to match the
available field data accurately and hence can be used as valuable
tools for predicting the future response of the systems to changes in
use.
Abstract: Although, arsenic trioxide has been the subject of
toxicological research, in vitro cytotoxicity and genotoxicity studies
using relevant cell models and uniform methodology are not well
elucidated. Hence, the aim of the present study was to evaluate the
cytotoxicity and genotoxicity induced by arsenic trioxide in human
keratinocytes (HaCaT) using the MTT [3-(4, 5-dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide] and alkaline single cell gel
electrophoresis (Comet) assays, respectively. Human keratinocytes
were treated with different doses of arsenic trioxide for 4 h prior to
cytogenetic assessment. Data obtained from the MTT assay indicated
that arsenic trioxide significantly reduced the viability of HaCaT cells
in a dose-dependent manner, showing an IC50 value of 34.18 ± 0.6
μM. Data generated from the comet assay also indicated a significant
dose-dependent increase in DNA damage in HaCaT cells associated
with arsenic trioxide exposure. We observed a significant increase in
comet tail length and tail moment, showing an evidence of arsenic
trioxide -induced genotoxic damage in HaCaT cells. This study
confirms that the comet assay is a sensitive and effective method to
detect DNA damage caused by arsenic.
Abstract: The concentration levels of six heavy metals (Cd, Cr,
Fe, Ni, Pb and Zn) and two mineral elements (Ca and Mg) were
determined in soil samples collected from the vicinity of two auto
mechanic workshops in Sabon-Gari, Kaduna state, Nigeria, using
Atomic Absorption Spectrometry (AAS), in order to compare the
gradation of their concentrations with distance and depth of soil from
the workshop sites. At site 1, concentrations of Lead, Chromium, Iron
and Zinc were generally found to be above the World Health
Organization limits, while those of Nickel and Cadmium fell within
the limits. Iron had the highest concentration with a range of 176.274
ppm to 489.127 ppm at depths of 5 cm to 15 cm and a distance range
of 5 m to 15 m, while the concentration of cadmium was least with a
range of 0.001 ppm to 0.008 ppm at similar depth and distance
ranges. In addition, there was more of calcium (11.521 ppm to
121.709 ppm), in all the samples, than magnesium (11.293 ppm to
21.635 ppm). Similar results were obtained for site II. The
concentrations of all the metals analyzed showed a downward
gradient with increase in depth and distance from both workshop sites
except for iron and zinc at site 2. The immediate and remote
implications of these findings on the biota are discussed.
Abstract: Geometric and mechanical properties all influence the
resistance of RC structures and may, in certain combination of
property values, increase the risk of a brittle failure of the whole
system.
This paper presents a statistical and probabilistic investigation on
the resistance of RC beams designed according to Eurocodes 2 and 8,
and subjected to multiple failure modes, under both the natural
variation of material properties and the uncertainty associated with
cross-section and transverse reinforcement geometry. A full
probabilistic model based on JCSS Probabilistic Model Code is
derived. Different beams are studied through material nonlinear
analysis via Monte Carlo simulations. The resistance model is
consistent with Eurocode 2. Both a multivariate statistical evaluation
and the data clustering analysis of outcomes are then performed.
Results show that the ultimate load behaviour of RC beams
subjected to flexural and shear failure modes seems to be mainly
influenced by the combination of the mechanical properties of both
longitudinal reinforcement and stirrups, and the tensile strength of
concrete, of which the latter appears to affect the overall response of
the system in a nonlinear way. The model uncertainty of the
resistance model used in the analysis plays undoubtedly an important
role in interpreting results.
Abstract: Urban areas have been expanded throughout the
globe. Monitoring and modelling urban growth have become a
necessity for a sustainable urban planning and decision making.
Urban prediction models are important tools for analyzing the causes
and consequences of urban land use dynamics. The objective of this
research paper is to analyze and model the urban change, which has
been occurred from 1990 to 2000 using CORINE land cover maps.
The model was developed using drivers of urban changes (such as
road distance, slope, etc.) under an Artificial Neural Network
modelling approach. Validation was achieved using a prediction map
for 2006 which was compared with a real map of Urban Atlas of
2006. The accuracy produced a Kappa index of agreement of 0,639
and a value of Cramer's V of 0,648. These encouraging results
indicate the importance of the developed urban growth prediction
model which using a set of available common biophysical drivers
could serve as a management tool for the assessment of urban
change.
Abstract: Analyzing DNA microarray data sets is a great
challenge, which faces the bioinformaticians due to the complication
of using statistical and machine learning techniques. The challenge
will be doubled if the microarray data sets contain missing data,
which happens regularly because these techniques cannot deal with
missing data. One of the most important data analysis process on
the microarray data set is feature selection. This process finds the
most important genes that affect certain disease. In this paper, we
introduce a technique for imputing the missing data in microarray
data sets while performing feature selection.
Abstract: Modelling of the earth's surface and evaluation of
urban environment, with 3D models, is an important research topic.
New stereo capabilities of high resolution optical satellites images,
such as the tri-stereo mode of Pleiades, combined with new image
matching algorithms, are now available and can be applied in urban
area analysis. In addition, photogrammetry software packages gained
new, more efficient matching algorithms, such as SGM, as well as
improved filters to deal with shadow areas, can achieve more dense
and more precise results.
This paper describes a comparison between 3D data extracted
from tri-stereo and dual stereo satellite images, combined with pixel
based matching and Wallis filter. The aim was to improve the
accuracy of 3D models especially in urban areas, in order to assess if
satellite images are appropriate for a rapid evaluation of urban
environments.
The results showed that 3D models achieved by Pleiades tri-stereo
outperformed, both in terms of accuracy and detail, the result
obtained from a Geo-eye pair. The assessment was made with
reference digital surface models derived from high resolution aerial
photography. This could mean that tri-stereo images can be
successfully used for the proposed urban change analyses.
Abstract: Modern low earth orbit (LEO) satellites that require multi-mission flexibility are highly likely to be repositioned between different operational orbits. While executing this process the satellite may experience high levels of vibration and environmental hazards, exposing the deployed solar panel to dangerous stress levels, fatigue and space debris, hence it is desirable to retract the solar array before satellite repositioning to avoid damage or failure.
A novel concept of deployable/retractable hybrid solar array systemcomposed of both rigid and flexible solar panels arranged within a petal formation, aimed to provide a greater power to volume ratio while dramatically reducing mass and cost is proposed.
Abstract: Fiber Bragg optic sensor is embedded in composite
material to detect and monitor the damage that occurs in composite
structures. In this paper, we deal with the mode-Ι delamination to
determine the material strength to crack propagation, using the
coupling mode theory and T-matrix method to simulate the FBGs
spectrum for both uniform and non-uniform strain distribution. The
double cantilever beam test is modeled in FEM to determine the
longitudinal strain. Two models are implemented, the first is the
global half model, and the second is the sub-model to represent the
FBGs with higher refined mesh. This method can simulate damage in
composite structures and converting strain to a wavelength shifting in
the FBG spectrum.
Abstract: Ocimum americanum L (Lamiaceae) is an annual herb
that is native to tropical Africa. The in vitro and in vivo antioxidant
activity of its aqueous extract was carefully investigated by assessing
the DPPH radical scavenging activity, ABTS radical scavenging
activity and hydrogen peroxide radical scavenging activity. The
reducing power, total phenol, total flavonoids and flavonols content
of the extract were also evaluated. The data obtained revealed that the
extract is rich in polyphenolic compounds and scavenged the radicals
in a concentration dependent manner. This was done in comparison
with the standard antioxidants such as BHT and Vitamin C. Also, the
induction of oxidative damage with paracetamol (2000 mg/kg)
resulted in the elevation of lipid peroxides and significant (P < 0.05)
decrease in activities of superoxide dismutase, glutathione
peroxidase, glutathione reductase and catalase in the liver and kidney
of rats. However, the pretreatment of rats with aqueous extract of O.
americanum leaves (200 and 400 mg/kg) and silymarin (100 mg/kg)
caused a significant (P < 0.05) reduction in the values of lipid
peroxides and restored the levels of antioxidant parameters in these
organs. These findings suggest that the leaves of O. americanum have
potent antioxidant properties which may be responsible for its
acclaimed folkloric uses.
Abstract: The present study was undertaken to investigate the
effect of aging parameters (time and temperature) on the mechanical
properties of Be-and/or Zr- treated Al-Mg-Zn (7075) alloys. Ultimate
tensile strength, 0.5% offset yield strength and % elongation
measurements were carried out on specimens prepared from cast and
heat treated 7075 alloys containing Be and/or Zr. Different aging
treatment were carried out for the as solution treated (SHT)
specimens (after quenching in warm water). The specimens were
aged at different conditions; Natural and artificial aging was carried
out at room temperature, 120C, 150C, 180C and 220C for different
periods of time. Duplex aging was performed for SHT conditions
(pre-aged at different time and temperature followed by high
temperature aging). Ultimate tensile strength, yield strength and %
elongation data results as a function of different aging parameters are
analysed. A statistical design of experiments (DOE) approach using
fractional factorial design is applied to acquire an understanding of
the effects of these variables and their interactions on the mechanical
properties of Be- and/or Zr- treated 7075 alloys. Mathematical
models are developed to relate the alloy mechanical properties with
the different aging parameters.
Abstract: Speech enhancement is a long standing problem with
numerous applications like teleconferencing, VoIP, hearing aids and
speech recognition. The motivation behind this research work is to
obtain a clean speech signal of higher quality by applying the optimal
noise cancellation technique. Real-time adaptive filtering algorithms
seem to be the best candidate among all categories of the speech
enhancement methods. In this paper, we propose a speech
enhancement method based on Recursive Least Squares (RLS)
adaptive filter of speech signals. Experiments were performed on
noisy data which was prepared by adding AWGN, Babble and Pink
noise to clean speech samples at -5dB, 0dB, 5dB and 10dB SNR
levels. We then compare the noise cancellation performance of
proposed RLS algorithm with existing NLMS algorithm in terms of
Mean Squared Error (MSE), Signal to Noise ratio (SNR) and SNR
Loss. Based on the performance evaluation, the proposed RLS
algorithm was found to be a better optimal noise cancellation
technique for speech signals.
Abstract: The aim of this study is to develop an anterior lumbar
interbody fusion (ALIF) PEEK cage suitable for Korean people. In this
study, CT images were obtained from Korean male (173cm, 71kg) and
3D Korean lumbar models were reconstructed based on the CT images
to investigate anatomical characteristics. Major design parameters of
anterior lumbar interbody fusion (ALIF) PEEK Cage were selected
using the morphological measurement information of the Korean
Lumbar models. Through finite element analysis and mechanical tests,
the developed ALIFPEEK Cage prototype was compared with the
Fidji Cage (Zimmer. Inc, USA) and it was found that the ALIF
prototype showed similar and/or superior mechanical performance
compared to the FidJi Cage. Also, clinical validation for the ALIF
PEEK Cage prototype was carried out to check predictable troubles in
surgical operations. Finally, it is considered that the convenience and
stability of the prototype was clinically verified.