Abstract: The two-dimensional gel electrophoresis method
(2-DE) is widely used in Proteomics to separate thousands of proteins
in a sample. By comparing the protein expression levels of proteins in
a normal sample with those in a diseased one, it is possible to identify
a meaningful set of marker proteins for the targeted disease. The major
shortcomings of this approach involve inherent noises and irregular
geometric distortions of spots observed in 2-DE images. Various
experimental conditions can be the major causes of these problems. In
the protein analysis of samples, these problems eventually lead to
incorrect conclusions. In order to minimize the influence of these
problems, this paper proposes a partition based pair extension method
that performs spot-matching on a set of gel images multiple times and
segregates more reliable mapping results which can improve the
accuracy of gel image analysis. The improved accuracy of the
proposed method is analyzed through various experiments on real
2-DE images of human liver tissues.