Abstract: Near-infrared (NIR) spectroscopy is a widely used
method for material identification for laboratory and industrial applications.
While standard spectrometers only allow measurements at
one sampling point at a time, NIR Spectral Imaging techniques can
measure, in real-time, both the size and shape of an object as well as
identify the material the object is made of. The online classification
and sorting of recovered paper with NIR Spectral Imaging (SI)
is used with success in the paper recycling industry throughout
Europe. Recently, the globalisation of the recycling material streams
caused that water-based flexographic-printed newspapers mainly from
UK and Italy appear also in central Europe. These flexo-printed
newspapers are not sufficiently de-inkable with the standard de-inking
process originally developed for offset-printed paper. This de-inking
process removes the ink from recovered paper and is the fundamental
processing step to produce high-quality paper from recovered paper.
Thus, the flexo-printed newspapers are a growing problem for the
recycling industry as they reduce the quality of the produced paper
if their amount exceeds a certain limit within the recovered paper
material.
This paper presents the results of a research project for the
development of an automated entry inspection system for recovered
paper that was jointly conducted by CTR AG (Austria) and PTS
Papiertechnische Stiftung (Germany). Within the project an NIR
SI prototype for the identification of flexo-printed newspaper has
been developed. The prototype can identify and sort out flexoprinted
newspapers in real-time and achieves a detection accuracy
for flexo-printed newspaper of over 95%. NIR SI, the technology the
prototype is based on, allows the development of inspection systems
for incoming goods in a paper production facility as well as industrial
sorting systems for recovered paper in the recycling industry in the
near future.
Abstract: Breast carcinoma is the most common form of cancer
in women. Multicolour fluorescent in-situ hybridisation (m-FISH) is
a common method for staging breast carcinoma. The interpretation
of m-FISH images is complicated due to two effects: (i) Spectral
overlap in the emission spectra of fluorochrome marked DNA probes
and (ii) tissue autofluorescence. In this paper hyper-spectral images of
m-FISH samples are used and spectral unmixing is applied to produce
false colour images with higher contrast and better information
content than standard RGB images. The spectral unmixing is realised
by combinations of: Orthogonal Projection Analysis (OPA), Alterating
Least Squares (ALS), Simple-to-use Interactive Self-Modeling
Mixture Analysis (SIMPLISMA) and VARIMAX. These are applied
on the data to reduce tissue autofluorescence and resolve the spectral
overlap in the emission spectra. The results show that spectral unmixing
methods reduce the intensity caused by tissue autofluorescence by
up to 78% and enhance image contrast by algorithmically reducing
the overlap of the emission spectra.