Abstract: Application of synthetic antioxidants such as tertbutylhydroquinon
(TBHQ), in spite of their efficiency, is questioned
because of their possible carcinogenic effect. The purpose of this
study was application of mixtures of natural antioxidants that provide
the best oxidative stability for margarine. Antioxidant treatments
included 10 various mixtures (F1- F10) containing 100-500ppm
tocopherol mixture (Toc), 100-200ppm ascorbyl palmitate (AP), 100-
200ppm rosemary extract (Ros) and 1000ppm lecithin(Lec) along
with a control or F0 (with no antioxidant) and F11 with 120ppm
TBHQ. The effect of antioxidant mixtures on the stability of
margarine samples during oven test (60°C), rancimat test at 110°C
and storage at 4°C was evaluated. Final ranking of natural antioxidant
mixtures was as follows: F2,F10>F5,F9>F8>F1,F3,F4>F6, F7.
Considering the results of this research and ranking criteria,
F2(200ppmAp + 200ppmRos) and F10(200ppmRos + 200ppmToc
+1000ppmLec) were recommended as substitutes for TBHQ to
maintain the quality and increase the shelf-life of margarine.