Long-term Monitor of Seawater by using TiO2:Ru Sensing Electrode for Hard Clam Cultivation

The hard clam (meretrix lusoria) cultivated industry has been developed vigorously for recent years in Taiwan, and seawater quality determines the cultivated environment. The pH concentration variation affects survival rate of meretrix lusoria immediately. In order to monitor seawater quality, solid-state sensing electrode of ruthenium-doped titanium dioxide (TiO2:Ru) is developed to measure hydrogen ion concentration in different cultivated solutions. Because the TiO2:Ru sensing electrode has high chemical stability and superior sensing characteristics, thus it is applied as a pH sensor. Response voltages of TiO2:Ru sensing electrode are readout by instrument amplifier in different sample solutions. Mean sensitivity and linearity of TiO2:Ru sensing electrode are 55.20 mV/pH and 0.999 from pH1 to pH13, respectively. We expect that the TiO2:Ru sensing electrode can be applied to real environment measurement, therefore we collect two sample solutions by different meretrix lusoria cultivated ponds in the Yunlin, Taiwan. The two sample solutions are both measured for 200 seconds after calibration of standard pH buffer solutions (pH7, pH8 and pH 9). Mean response voltages of sample 1 and sample 2 are -178.758 mV (Standard deviation=0.427 mV) and -180.206 mV (Standard deviation =0.399 mV), respectively. Response voltages of the two sample solutions are between pH 8 and pH 9 which conform to weak alkali range and suitable meretrix lusoria growth. For long-term monitoring, drift of cultivated solutions (sample 1 and sample 2) are 1.16 mV/hour and 1.03 mV/hour, respectively.

Functional Sample of the Portable Device for Fast Analysis of Explosives

The construction of original functional sample of the portable device for fast analysis of energetic materials has been described in the paper. The portable device consisting of two parts – an original miniaturized microcolumn liquid chromatograph and a unique chemiluminescence detector – has been proposed and realized. In a very short time, this portable device is capable of identifying selectively most of military nitramine- and nitroesterbased explosives as well as inorganic nitrates occurring in trace concentrations in water or in soil. The total time required for the identification of extracts is shorter than 8 minutes.

Inventory and Characterization of Selected Deep Sea Fish Species as an Alternative Food Source from Southern Java Ocean and Western Sumatra Ocean, Indonesia

Sixteen selected deep-sea fish obtained from Southern Java Ocean and Western Sumatra Ocean was analyzed to determine its proximate, fatty acid and mineral composition. The moisture content was ranged from 64.38 to 86.04 %, ash from 0.17 to 0.69 %, the fat content was 1.54 – 13.30 % while the protein content varied from 15.84 to 23.60%. Among the fatty acids, oleic acid and palmitic acid was the dominant MUFA and SFA. Linoleic acid was the highest PUFA found at the selected deep-sea fish. Phospor was the highest macroelement concentration on selected deep-sea fish, followed by K, Ca, Mg and Iod, Fe and Zn among microelement. The trace concentration was found at Se microelement.

Sterilisation of in vitro Culture Medium of Chrysanthemum by Plant Essential Oils without Autoclaving

The alternative technique for sterilization of culture medium to replace autoclaving was carried out. For sterilization of culture medium without autoclaving, some commercial pure essential oils, bergamot oil, betel oil, cinnamon oil, lavender oil and turmeric oil, were tested alone or in combinations with some disinfectants, 10% povidone-iodine and 2% iodine + 2.4% potassium iodide. Each essential oil or combination was added to 25-mL Murashige and Skoog (MS) medium before medium was solidified in a 120-mL container, kept for 2 weeks before evaluating sterile conditions. Treated media, supplemented with essential oils, were compared to control medium, autoclaved at 121 degree Celsius for 15 min. In vitro sterile conditions were found 20 – 100% from these treated media compared to 100% sterile condition from autoclaved medium. Treated media obtained 100% sterile conditions were chosen for culturing chrysanthemum shoots. It was found that 10% povidoneiodine in combination with cinnamon oil (3:1) and 2% iodine + 2.4% potassium iodide in combination with lavender oil (1:3) at the concentration of 36 3L/25 mL medium provided the promising growth of shoot explants.

SMEs Relationship Banking: Length, Loyalty, Trust. Do SMEs get Something in Return?

Under the difficult access to finance of SMEs, they expect that its relationship with the banks shall constitute a real help to access appropriate financing, at reasonable costs and requirements, given the possibility of mutually beneficial and long lasting relation. The literature, but also the research we have carried on, is centered on such determinants as concentration and the length of the relationship, but at the same time, there is little certainty that banks are responding positively to them. Furthermore, although the trust is considered as being a fundamental element of bank relationship – see the case house bank – SMEs find that the banks finance them looking rather on collaterals and covenants than to trust. Moreover, a positive behavior, such as prompt or advance repayments of loans, doesn-t generate any positive feedback from the banks side. All these show a deep un-satisfaction of the SMEs concerning their relationship banking.

Microbiological Assessment of Yoghurt Enriched with Flakes from Barley Grain and Malt Extract during Shelf-Life

The effect of flakes from biologically activated hullless barley grain and malt extract on microbiological safety of yoghurt was studied. Pasteurized milk, freeze-dried yoghurt culture YF-L811 (Chr. Hansen, Denmark), flakes from biologically activated hull-less barley grain (Latvia) and malt extract (Ilgezeem, Latvia) were used for experiments. Yoghurt samples with flakes from biologically activated hull-less barley grain and malt extract were analyzed for total plate count of mesophylic aerobic and facultative anaerobic microorganisms, as well yeasts and moulds population during shelflife. Results showed that the changes of pH and titratable acidity affected the concentration of added malt extract. The lowest pH and the highest titratable acidity were determined in samples YFBG5% ME4% and YFBG5% ME6% on the 14th day. The total plate count decreased in all yoghurt samples except sample YFBG5% ME6%, where was determined the increase of microorganisms from 7th till 14th day. The adding of flakes from biologically activated hull-less barley grain in yoghurt samples caused the higher initial content of yeasts and moulds comparing with control. The growth of yeasts and moulds during shelf-life provided the added malt extract in yoghurt samples. Yoghurt enriched with flakes from biologically activated hull-less barley grain and malt extract from a microbiological perspective is safe product.

The Effect of Pleurotus sajor-caju (PSC) Addition on the Nutritional Composition and Sensory Properties of Poultry-Based Patty

The nutrient composition and sensory properties of poultry-based patties (PBPs) incorporated with various levels of grey oyster mushroom (Pleurotus sajor-caju, PSC) were studied. The PBPs were formulated with either 0%, 25% or 50% of fresh ground PSC. Results show poultry patty formulated with 25% PSC had protein content of 17.46% lower than the control patty which had 18.13% but it was not significant. Meanwhile, both cooked poultry patties containing 25% and 50% PSC significantly recorded lower concentration of fat at 10.67% and 7.15%, respectively. On the other hand, poultry patty added with 50% ground PSC shows the highest concentration of total dietary fibre (TDF) of 4.90 g/100g compared to poultry patty containing 25% of mushroom (3.40 g/100g) and to the control (1.90g/100g). In addition, patty incorporated with 25% PSC had moisture content of 57.91% which is significantly lower than patty formulated with 50% which had moisture of 61.80%. In the sensory evaluation, there were no differences recorded in all sensory attributes of PSC-based patties judged by untrained panelists. In conclusion, the addition of PSC to replace poultry meat can be recommended for the purpose of lowering production cost, enhancing nutritional composition and maintaining the acceptability of poultry patties.

Evaluation of Chromium Contamination in the Sediments of Jen-Gen River Mouth, Taiwan

This study was conducted using the data collected at the mouth of Jen-Gen River to investigate and analyze chromium (Cr) contained in the sediments, and to evaluate the accumulation of Cr and the degree of its potential risk. The results show that samples collected at all monitoring stations near the mouth of Jen-Gen River contain 92–567 mg/kg of Cr with average of 366±166 mg/kg. The spatial distribution of Cr reveals that the Cr concentration is relatively high in the river mouth region, and gradually diminishes toward the harbor region. This indicates that upstream industrial and municipal wastewater discharges along the river bank are major sources of pollution. The accumulation factor and potential ecological risk index indicate that the sedimentation at Jen-Gen River mouth has the most serious degree of Cr accumulation and the highest ecological potential risk.

Osmotic Dehydration of Apricot using Saltsucrose Solutions

Fruit drying is a well known process mostly used for preservation of fruits. Osmotic dehydration of apricot slices were carried out in three different salt-sucrose concentrations and four different temperatures. Also three different weight ratios of solution to sample were conducted to one set of experiments. The dehydration curves were constructed using Peleg-s model. Increasing the solution volume increased the mass transfer rate and hence the solid gain increased rapidly. Increasing the volume of osmotic media caused an increase in overall mass transfer but a 'solution to sample' ratio of 5:1 gave the best product quality. The best temperature and concentration that had a high water loss to solid gain ratio and an acceptable taste were 40°C and 5%, respectively.

Pomelo Peel: Agricultural Waste for Biosorption of Cadmium Ions from Aqueous Solutions

The ability of pomelo peel, a natural biosorbent, to remove Cd(II) ions from aqueous solution by biosorption was investigated. The experiments were carried out by batch method at 25 °C. The influence of solution pH, initial cadmium ion concentrations and contact times were evaluated. Cadmium ion removal increased significantly as the pH of the solution increased from pH 1 to pH 5. At pH 5, the cadmium ion removal reached a maximum value. The equilibrium process was described well by the Langmuir isotherm model, with a maximum biosorption capacity of 21.83 mg/g. The biosorption was relatively quick, (approx. 20 min). Biosorption kinetics followed a pseudo-second-order model. The result showed that pomelo peel was effective as a biosorbent for removing cadmium ions from aqueous solution. It is a low cost material that shows potential to be applied in wastewater technology for remediation of heavy metal contamination.

Deicing and Corrosive Performances of Calcium Acetate Deicer Made from Bamboo-Vinegar

Calcium magnesium acetate (CMA) is environmentally benign deicing chemicals that can replace sodium chloride that is widely used on roads and highways at present for snow and ice control to provide safe driving conditions during winter. The price of CMA from petroleum-derived acetic acid is quite expensive. The bamboo vinegar is the by-product from bamboo charcoal production. The bamboo vinegar was used to prepare calcium acetate as raw materials, and its deicing and corrosive performances were studied in this paper. The results show that the freezing temperature of calcium acetate is lower than that of sodium chloride when they have same molar concentration, the deicing performance of calcium acetate is better than that of sodium chloride when they have same moles, while the deicing performance of sodium chloride is better than that of calcium acetate. The corrosion of sodium chloride on iron-nail and steel-nail is larger than that of calcium acetate whether they have same mass concentration or same molar concentration, and the corrosion of sodium chloride and calcium acetate on iron-nail is larger than that on steel-nail, and calcium acetate almost hasn't corrosion on steel-nail.

Study of Aluminum, Copper and Molybdenum Pollution in Groundwater Sources Surrounding (Miduk) Shahr-E- Babak Copper Complex Tailings Dam

Interpolated contour maps drawn for aluminum, copper and molybdenum in downstream monitoring boreholes of water dam in Miduk Copper Complex and the values of pH, redox potential (Eh) and distance from water dam indicate different trends of variation and behavior of these three elements in downward groundwater resources. As these maps exhibit, aluminum is dominant in the most alkaline (pH = 9-11) borehole (MB5) to water dam. The highest concentration of molybdenum is found in the nearest borehole (MB6) to water dam. Main concentration of copper is observed in the most oxidized borehole (MB3 with Eh=293.2mV). The spatial difference among sampling stations can be attributed to the existence of faults and diaclases in the geologic structure of Miduk region which causes the groundwater sampling sites to be impressed by different contamination sources (toe seepage and upper seepage water originated from different zones of tailings dump).

Effect of Transglutaminase Cross Linking on the Functional Properties as a Function of NaCl Concentration of Legumes Protein Isolate

The effect of cross linking of the protein isolates of three legumes with the microbial enzyme transglutaminase (EC 2.3.2.13) on the functional properties at different NaCl concentration was studied. The reduction in the total free amino groups (OD340) of the polymerized protein showed that TGase treatment cross-linking the protein subunit of each legume. The solubility of the protein polymer of each legume was greatly improved at high concentration of NaCl. At 1.2 M NaCl the solubility of the native legumes protein was significantly decreased but after polymerization slightly improved. Cross linked proteins were less turbid on heating to higher temperature as compared to native proteins and the temperature at which the protein turns turbid also increased in the polymerized proteins. The emulsifying and foaming properties of the protein polymer were greatly improved at all concentrations of NaCl for all legumes.

The Possibility Distribution for the Controlled Bloodstream Concentrations of Any Physiologically Active Substance

In many ways, biomedical analysis is analogous to possibilistic reasoning. In spite of that, there are hardly any applications of possibility theory in biology or medicine. The aim of this work is to demonstrate the use of possibility theory in an epidemiological study. In the paper, we build the possibility distribution for the controlled bloodstream concentrations of any physiologically active substance through few approximate considerations. This possibility distribution is tested later against the empirical histograms obtained from the panel study of the eight different physiologically active substances in 417 individuals.

Investigation on Toxicity of Manufactured Nanoparticles to Bioluminescence Bacteria Vibrio fischeri

Acute toxicity of nano SiO2, ZnO, MCM-41 (Meso pore silica), Cu, Multi Wall Carbon Nano Tube (MWCNT), Single Wall Carbon Nano Tube (SWCNT) , Fe (Coated) to bacteria Vibrio fischeri using a homemade luminometer , was evaluated. The values of the nominal effective concentrations (EC), causing 20% and 50% inhibition of biouminescence, using two mathematical models at two times of 5 and 30 minutes were calculated. Luminometer was designed with Photomultiplier (PMT) detector. Luminol chemiluminescence reaction was carried out for the calibration graph. In the linear calibration range, the correlation coefficients and coefficient of Variation (CV) were 0.988 and 3.21% respectively which demonstrate the accuracy and reproducibility of the instrument that are suitable. The important part of this research depends on how to optimize the best condition for maximum bioluminescence. The culture of Vibrio fischeri with optimal conditions in liquid media, were stirring at 120 rpm at a temperature of 150C to 180C and were incubated for 24 to 72 hours while solid medium was held at 180C and for 48 hours. Suspension of nanoparticles ZnO, after 30 min contact time to bacteria Vibrio fischeri, showed the highest toxicity while SiO2 nanoparticles showed the lowest toxicity. After 5 min exposure time, the toxicity of ZnO was the strongest and MCM-41 was the weakest toxicant component.

Climate Change Effect from Black Carbon Emission: Open Burning of Corn Residues in Thailand

This study focuses on emission of black carbon (BC) from field open burning of corn residues. Real-time BC concentration was measured by Micro Aethalometer from field burning and simulated open burning in a chamber (SOC) experiments. The average concentration of BC was 1.18±0.47 mg/m3 in the field and 0.89±0.63 mg/m3 in the SOC. The deduced emission factor from field experiments was 0.50±0.20 gBC/kgdm, and 0.56±0.33 gBC/kgdm from SOC experiment, which are in good agreement with other studies. In 2007, the total burned area of corn crop was 8,000 ha, resulting in an emission load of BC 20 ton corresponding to 44.5 million kg CO2 equivalent. Therefore, the control of open burning in corn field represents a significant global warming reduction option.

Hazards Assessment of Radon Exhalation Rate and Radium Content in the Soil Samples in Iraqi Kurdistan Using Passive and Active Detecting Methods

This study aims to assess the environmental hazards from radon exhalation rate in the soil samples in selected locations in Iraqi Kurdistan, using passive (CR-39NTDs) and active (RAD7) detecting method. Radon concentration, effective radium content and radon exhalation rate were estimated in soil samples that collected at the depth level of 30 cm inside 124 houses. The results show that the emanation rate for radon gas was variation from location to other, depending on the geological formation. Most health risks come from emanation of radon and its daughter due to its contribution for indoor radon, so the results showed that there is a linear relationship between the ratio of soil and indoor radon concentration (CSoil Rn222/ Cindoor Rn222) and the effective radium content in soil samples. The results show that radon concentration has high and low values in Hajyawa city and Er. Tyrawa Qr, respectively. A comparison between our results with that mentioned in international reports was done.

Preliminary Investigation on Combustion Characteristics of Rice Husk in FBC

The experimental results on combustion of rice husk in a conical fluidized bed combustor (referred to as the conical FBC) using silica sand as the bed material are presented in this paper. The effects of excess combustion air and combustor loading as well as the sand bed height on the combustion pattern in FBC were investigated. Temperatures and gas concentrations (CO and NO) along over the combustor height as well as in the flue gas downstream from the ash collecting cyclone were measured. The results showed that the axial temperature profiles in FBC were explicitly affected by the combustor loading whereas the excess air and bed height were found to have minor influences on the temperature pattern. Meanwhile, the combustor loading and the excess air significantly affected the axial CO and NO concentration profiles; however, these profiles were almost independent of the bed height. The combustion and thermal efficiencies for this FBC were quantified for different operating conditions.

Bioprocessing of Proximally Analyzed Wheat Straw for Enhanced Cellulase Production through Process Optimization with Trichodermaviride under SSF

The purpose of the present work was to study the production and process parameters optimization for the synthesis of cellulase from Trichoderma viride in solid state fermentation (SSF) using an agricultural wheat straw as substrates; as fungal conversion of lignocellulosic biomass for cellulase production is one among the major increasing demand for various biotechnological applications. An optimization of process parameters is a necessary step to get higher yield of product. Several kinetic parameters like pretreatment, extraction solvent, substrate concentration, initial moisture content, pH, incubation temperature and inoculum size were optimized for enhanced production of third most demanded industrially important cellulase. The maximum cellulase enzyme activity 398.10±2.43 μM/mL/min was achieved when proximally analyzed lignocellulosic substrate wheat straw inocubated at 2% HCl as pretreatment tool along with distilled water as extraction solvent, 3% substrate concentration 40% moisture content with optimum pH 5.5 at 45°C incubation temperature and 10% inoculum size.