Abstract: The purpose of the present work was to study the
production and process parameters optimization for the synthesis of
cellulase from Trichoderma viride in solid state fermentation (SSF)
using an agricultural wheat straw as substrates; as fungal conversion
of lignocellulosic biomass for cellulase production is one among the
major increasing demand for various biotechnological applications.
An optimization of process parameters is a necessary step to get
higher yield of product. Several kinetic parameters like pretreatment,
extraction solvent, substrate concentration, initial moisture content,
pH, incubation temperature and inoculum size were optimized for
enhanced production of third most demanded industrially important
cellulase. The maximum cellulase enzyme activity 398.10±2.43
μM/mL/min was achieved when proximally analyzed lignocellulosic
substrate wheat straw inocubated at 2% HCl as pretreatment tool
along with distilled water as extraction solvent, 3% substrate
concentration 40% moisture content with optimum pH 5.5 at 45°C
incubation temperature and 10% inoculum size.
Abstract: The present study was conducted to observe the effect
of Plantago psyllium on blood glucose and cholesterol levels in
normal and alloxan induced diabetic rats. To investigate the effect of
Plantago psyllium 40 rats were included in this study divided into
four groups of ten rats in each group. One group A was normal,
second group B was diabetic, third group C was non diabetic and
hypercholesterolemic and fourth group D was diabetic and
hypercholesterolemic. Two groups B and D were made diabetic by
intraperitonial injection of alloxan dissolved in 1mL distilled water at
a dose of 125mg/Kg of body weight. Two groups C and D were
made hypercholesterolemic by oral administration of powder
cholesterol (1g/Kg of body weight). The blood samples from all the
rats were collected from coccygial vein on 1st day, then on 21st and
42nd day respectively. All the samples were analyzed for blood
glucose and cholesterol level by using enzymatic kits. The blood
glucose and cholesterol levels of treated groups of rats showed
significant reduction after 7 weeks of treatment with Plantago
psyllium. By statistical analysis of results it was found that Plantago
psyllium has anti-diabetic and hypocholesterolemic activity in
diabetic and hypercholesterolemic albino rats.
Abstract: Rhizopus oligosporus was used in the present study
for the production of protease enzyme under SSF. Sunflower meal
was used as by-product of oil industry incorporated with organic salts
was employed for the production of protease enzyme. The main
purpose of the present was to study different parameters of protease
productivity, its yields and to optimize basal fermentation conditions.
The optimal conditions found for protease production using
sunflower meal as a substrate in the present study were inoculum size
(1%), substrate (Sunflower meal), substrate concentration (20 g), pH
(3), cultivation period (72 h), incubation temperature (35oC),
substrate to diluent-s ratio (1:2) and tween 81 (1 mL). The maximum
production of protease in the presence of cheaper substrate at low
concentration and stability at acidic pH, these characteristics make
the strain and its enzymes useful in different industry.