Abstract: β-Glucosidase is an important enzyme for production
of ethanol from lignocellulose. With hydrolytic activity on
cellooligosaccharides, especially cellobiose, β-glucosidase removes
product inhibitory effect on cellulases and forms fermentable sugars.
In this study, β-glucosidase encoding gene (BGL1) from traditional
starter yeast Saccharomycosis fibuligera BMQ908 was cloned and
expressed in Pichia pastoris. BGL1 of S. fibuligera BMQ 908 shared
98% nucleotide homology with the closest GenBank sequence
(M22475) but identity in amino-acid sequences of catalytic domains.
Recombinant plasmid pPICZαA/BGL1 containing the sequence
encoding BGL1 mature protein and α-factor secretion signal was
constructed and transformed into methylotrophic yeast P. pastoris by
electroporation. The recombinant strain produced single extracellular
protein with molecular weight of 120 kDa and cellobiase activity of
60 IU/ml. The optimum pH of the recombinant β-glucosidase was 5.0
and the optimum temperature was 50°C.
Abstract: A procedure for the preparation of clarified Pawpaw
Juice was developed. About 750ml Pawpaw pulp was measured into
2 measuring cylinders A & B of capacity 1 litre heated to 400C,
cooled to 200C. 30mls pectinase was added into cylinder A, while
30mls distilled water was added into cylinder B. Enzyme treated
sample (A) was allowed to digest for 5hours after which it was heated
to 900C for 15 minutes to inactivate the enzyme. The heated sample
was cooled and with the aid of a mucillin cloth the pulp was filtered
to obtain the clarified pawpaw juice. The juice was filled into 100ml
plastic bottles, pasteurized at 950C for 45 minutes, cooled and stored
at room temperature. The sample treated with 30mls distilled water
also underwent the same process. Freshly pasteurized sample was
analyzed for specific gravity, titratable acidity, pH, sugars and
ascorbic acid. The remaining sample was then stored for 2 weeks and
the above analyses repeated. There were differences in the results of
the freshly pasteurized samples and stored sample in pH and ascorbic
acid levels, also sample treated with pectinase yielded higher
volumes of juice than that treated with distilled water.
Abstract: In Algeria, some fruit trees produce fruits in free nature. Such trees are Celtis australis, Crataegus azarolus, Crataegus monogyna and Zizyphus lotus. In spite of their appreciable consumption, their nutritional value remains unknown. The objective of this study is the determination of sugars in the pulpe and almond of the above fruits. The biochemical analysis shows that these fruits present interesting contents of soluble sugars which confers significant caloric intakes to them. As well as significant fibres which give them therapeutic and industrial benefits? The analysis of the almonds shows that it contains considerable contents of sugars which enable them to be an energetic food.