Abstract: Nanotherapy is an actual newest mode of treatment numerous diseases using nanoparticles (NPs) loading with different pharmaceuticals. NPs of biodegradable polymeric micelles (PMs) are gaining increased attention for their numerous and attractive abilities to be used in a variety of applications in the various fields of medicine. The present paper deals with the synthesis of a class of biodegradable micelle-forming polymers, namely ABA triblock-copolymer in which A-blocks represent amino-poly(ethylene glycol) (H2N-PEG) and B-block is biodegradable amino acid-based poly(ester amide) constituted of α-amino acid – L-phenylalanine. The obtained copolymer formed micelles of 70±4 nm size at 10 mg/mL concentration.
Abstract: This work presents synthesis of α,ω-dithienyl
terminated poly(ethylene glycol) (PEGTh) capable for further chain
extension by either chemical or electrochemical polymeriztion.
PEGTh was characterized by FTIR and 1H-NMR. Further
copolymerization of PEGTh and pyrrole (Py) was performed by
chemical oxidative polymerization using ceric (IV) salt as an oxidant
(PPy-PEGTh). PEG without end group modification was used
directly to prepare copolymers with Py by Ce (IV) salt (PPy-PEG).
Block copolymers with mole ratio of pyrrole to PEGTh (PEG) 50:1
and 10:1 were synthesized. The electrical conductivities of
copolymers PPy-PEGTh and PPy-PEG were determined by four
point probe technique. Influence of the synthetic route and content of
the insulating segment on conductivity and yield of the copolymers
were investigated.
Abstract: Nonspecific protein adsorption generally occurs on
any solid surfaces and usually has adverse consequences. Adsorption
of proteins onto a solid surface is believed to be the initial and
controlling step in biofouling. Surfaces modified with end-tethered
poly(ethylene glycol) (PEG) have been shown to be protein-resistant
to some degree. In this study, the adsorption of β-casein and
lysozyme was performed on 6 different types of surfaces where PEG
was tethered onto stainless steel by polyethylene imine (PEI) through
either OH or NHS end groups. Protein adsorption was also performed
on the bare stainless steel surface as a control. The adsorption was
conducted at 23 °C and pH 7.2. In situ QCM-D was used to
determine PEG adsorption kinetics, plateau PEG chain densities,
protein adsorption kinetics and plateau protein adsorbed quantities.
PEG grafting density was the highest for a NHS coupled chain,
around 0.5 chains / nm2. Interestingly, lysozyme which has smaller
size than β-casein, appeared to adsorb much less mass than that of β-
casein. Overall, the surface with high PEG grafting density exhibited
a good protein rejection.