Abstract: An alarming emergence of multidrug-resistant strains
of the tuberculosis pathogen Mycobacterium tuberculosis and
continuing high worldwide incidence of tuberculosis has invigorated
the search for novel drug targets. The enzyme glutamate racemase
(MurI) in bacteria catalyzes the stereoconversion of L-glutamate to
D-glutamate which is a component of the peptidoglycan cell wall of
the bacterium. The inhibitors targeted against MurI from several
bacterial species have been patented and are advocated as promising
antibacterial agents. However there are none available against MurI
from Mycobacterium tuberculosis, due to the lack of its threedimensional
structure. This work accomplished two major objectives.
First, the tertiary structure of MtMurI was deduced computationally
through homology modeling using the templates from bacterial
homologues. It is speculated that like in other Gram-positive bacteria,
MtMurI exists as a dimer and many of the protein interactions at the
dimer interface are also conserved. Second, potent candidate
inhibitors against MtMurI were identified through docking against
already known inhibitors in other organisms.
Abstract: The most common result of analysis of highthroughput
data in molecular biology represents a global list of
genes, ranked accordingly to a certain score. The score can be a
measure of differential expression. Recent work proposed a new
method for selecting a number of genes in a ranked gene list from
microarray gene expression data such that this set forms the
Optimally Functionally Enriched Network (OFTEN), formed by
known physical interactions between genes or their products. Here
we present calculation results of relative connectivity of genes from
META-OFTEN network and tentative biological interpretation of the
most reproducible signal. The relative connectivity and
inbetweenness values of genes from META-OFTEN network were
estimated.
Abstract: In this paper, a nonlinear model predictive swing-up
and stabilizing sliding controller is proposed for an inverted
pendulum-cart system. In the swing up phase, the nonlinear model
predictive control is formulated as a nonlinear programming problem
with energy based objective function. By solving this problem at
each sampling instant, a sequence of control inputs that optimize the
nonlinear objective function subject to various constraints over a
finite horizon are obtained. Then, this control drives the pendulum to
a predefined neighborhood of the upper equilibrium point, at where
sliding mode based model predictive control is used to stabilize the
systems with the specified constraints. It is shown by the simulations
that, due to the way of formulating the problem, short horizon
lengths are sufficient for attaining the swing up goal.
Abstract: An empirical study of web applications that use
software frameworks is presented here. The analysis is based on two
approaches. In the first, developers using such frameworks are
required, based on their experience, to assign weights to parameters
such as database connection. In the second approach, a performance
testing tool, OpenSTA, is used to compute start time and other such
measures. From such an analysis, it is concluded that open source
software is superior to proprietary software. The motivation behind
this research is to examine ways in which a quantitative assessment
can be made of software in general and frameworks in particular.
Concepts such as metrics and architectural styles are discussed along
with previously published research.
Abstract: A new digital transceiver circuit for asynchronous frame detection is proposed where both the transmitter and receiver contain all digital components, thereby avoiding possible use of conventional devices like monostable multivibrators with unstable external components such as resistances and capacitances. The proposed receiver circuit, in particular, uses a combinational logic block yielding an output which changes its state as soon as the start bit of a new frame is detected. This, in turn, helps in generating an efficient receiver sampling clock. A data latching circuit is also used in the receiver to latch the recovered data bits in any new frame. The proposed receiver structure is also extended from 4- bit information to any general n data bits within a frame with a common expression for the output of the combinational logic block. Performance of the proposed hardware design is evaluated in terms of time delay, reliability and robustness in comparison with the standard schemes using monostable multivibrators. It is observed from hardware implementation that the proposed circuit achieves almost 33 percent speed up over any conventional circuit.