Abstract: Ethanol has been known for a long time, being
perhaps the oldest product obtained through traditional biotechnology
fermentation. Agriculture waste as substrate in fermentation is vastly
discussed as alternative to replace edible food and utilization of
organic material. Pineapple peel, highly potential source as substrate
is a by-product of the pineapple processing industry. Bio-ethanol
from pineapple (Ananas comosus) peel extract was carried out by
controlling fermentation without any treatment. Saccharomyces
ellipsoides was used as inoculum in this fermentation process as it is
naturally found at the pineapple skin. In this study, the capability of
Response Surface Methodology (RSM) for optimization of ethanol
production from pineapple peel extract using Saccharomyces
ellipsoideus in batch fermentation process was investigated. Effect of
five test variables in a defined range of inoculum concentration 6-
14% (v/v), pH (4.0-6.0), sugar concentration (14-22°Brix),
temperature (24-32°C) and time of incubation (30-54 hrs) on the
ethanol production were evaluated. Data obtained from experiment
were analyzed with RSM of MINITAB Software (Version 15)
whereby optimum ethanol concentration of 8.637% (v/v) was
determined. The optimum condition of 14% (v/v) inoculum
concentration, pH 6, 22°Brix, 26°C and 30hours of incubation. The
significant regression equation or model at the 5% level with
correlation value of 99.96% was also obtained.
Abstract: The present study presents a new approach to automatic
data clustering and classification problems in large and complex
databases and, at the same time, derives specific types of explicit rules
describing each cluster. The method works well in both sparse and
dense multidimensional data spaces. The members of the data space
can be of the same nature or represent different classes. A number
of N-dimensional ellipsoids are used for enclosing the data clouds.
Due to the geometry of an ellipsoid and its free rotation in space
the detection of clusters becomes very efficient. The method is based
on genetic algorithms that are used for the optimization of location,
orientation and geometric characteristics of the hyper-ellipsoids. The
proposed approach can serve as a basis for the development of
general knowledge systems for discovering hidden knowledge and
unexpected patterns and rules in various large databases.